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Transforming growth factor-beta: Signal transduction via protein kinase C in cultured embryonic vascular smooth muscle cells

  • Growth Differentiation And Senescence
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Summary

Transforming growth factor-beta (TGF-β), an ubiquitous regulatory peptide, has diverse effects on the differentiation and behavior of vascular smooth muscle cells (VSMC). However, the molecular mechanism through which TGF-α exerts its effects remains obscure. We investigated the phosphoinositide/protein kinase C [PKC] signaling pathway in the action of TGF-β on cultured embryonic avian VSMC of differing lineage: a) thoracic aorta, derived from the neural crest; and b) abdominal aorta, derived from mesenchyme. The second messenger responsible for activation of PKC is sn-1,2-diacylglycerol [DAG]; TGF-β increased the mass amounts of DAG in the membranes of neural crest-derived VSMC concurrent with translocation of PKC from the soluble to the membrane fraction, but TGF-β had no effect on the DAG or PKC of mesenchyme-derived VSMC. TGF-β potentiated the growth of platelet-derived growth factor (PDGF)-treated, neural crest-derived VSMC; but abolished PDGF-induced growth of mesenchymal cells. It is concluded that molecular and functional responses of VSMC to TGF-β are heterogeneous and are functions of the embryonic lineage of the VSMC.

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Author notes

  1. Thomas H. Rosenquist

    Present address: Department of Cell Biology and Anatomy, University of Nebraska School of Medicine, 600 S. 42nd, 68198, Omaha, Nebraska

Authors and Affiliations

  1. Department of Cellular Biology and Anatomy, Schools of Medicine and Biomedical Graduate Studies, Medical College of Georgia, 30912, Augusta, Georgia

    Robert W. Wrenn, Claire L. Raeuber, Lee E. Herman, Wendy J. Walton & Thomas H. Rosenquist

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Wrenn, R.W., Raeuber, C.L., Herman, L.E. et al. Transforming growth factor-beta: Signal transduction via protein kinase C in cultured embryonic vascular smooth muscle cells. In Vitro Cell Dev Biol - Animal 29, 73–78 (1993). https://doi.org/10.1007/BF02634374

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  • DOI: https://doi.org/10.1007/BF02634374

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