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Thymidine kinase-deficient mutants ofaedes albopictus mosquito cells

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Summary

Aedes albopictus mosquito cells resistant to the thymidine analog 5-bromodeoxyuridine (BrdU) were obtained using a single-step selection procedure. The resistant cells were characterized with respect to growth in the presence of BrdU, incorporation of [3H]uridine and [3H]thymidine, and thymidine kinase activity in crude extracts. The LC50 for TK-6 cells was 95µg BrdU/ml, and for TK-8 cells was 45µg/ml. Both clones incorporated [3H]uridine at levels corresponding to those in wild-type cells. TK-6 and TK-8 cells did not incorporate [3H]thymidine into acid-precipitable material, nor did they contain measurable thymidine kinase activity. Thymidine kinase activity in crude extracts from wild-type cells had a Km of 2µM and a Vmax of 10 pmol · min−1 · mg−1 protein.

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Authors and Affiliations

  1. Department of Entomology, University of Minnesota, 219 Hodson Hall, 1980 Folwell Avenue, 55108, St. Paul, Minnesota

    Celeste A. Mazzacano & Ann M. Fallon

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  1. Celeste A. Mazzacano
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  2. Ann M. Fallon
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Mazzacano, C.A., Fallon, A.M. Thymidine kinase-deficient mutants ofaedes albopictus mosquito cells. In Vitro Cell Dev Biol - Animal 28, 455–458 (1992). https://doi.org/10.1007/BF02634051

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  • DOI: https://doi.org/10.1007/BF02634051

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