Abstract
We report here the first isolation and characterization of drug-resistant cell lines from cultured mosquito cells. A BrdU-resistant cell line was isolated from mutagen-treatedAedes albopictus cells, and then used as the parent cell from which sublines resistant to ouabain or α-amanitin were derived. The BrdU-resistant cell line and all sublines derived from it were shown to be deficient in thymidine kinase. Evidence that favored attributing the drug-resistant phenotypes to mutant genotypes included (a) the drug-resistant phenotypes were stable for up to 16 months of culture under nonselective conditions, and (b) the frequency of the ouabain-resistant cells in a sensitive population was increased 500-fold (to between 10−3 and 10−4) by exposure to the chemical mutagen EMS. Evidence illustrating the usefulness of mutant mosquito cells for the study of the genetics of somatic cells is discussed.
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The work reported in this paper will be included in the dissertation to be submitted by S. J. M. in partial fulfillment of the requirements for the Ph.D. degree in Microbiology awarded by the Graduate School of Rutgers University.
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Mento, S.J., Stollar, V. Isolation and partial characterization of drug-resistantAedes albopictus cells. Somat Cell Mol Genet 4, 179–191 (1978). https://doi.org/10.1007/BF01538983
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DOI: https://doi.org/10.1007/BF01538983