Summary
Sugar in the medium is considered to be an essential cause for the high production costs of plantlets in conventional, heterotrophic micropropagation. Chlorophyllous explants, shoots, and plantlets in vitro have high photosynthetic ability to develop photoautotrophy, but their photosynthetic activity is restricted largely by the low CO2 concentration in the vessel during the photoperiod and in part by the presence of sugar in the medium. The growth of plantlets in vitro is often greater under photoautotrophic conditions than under heterotrophic conditions, provided that the in vitro environment is properly controlled for promoting photosynthesis. The advantages and disadvantages of photoautotrophic micropropagation are discussed.
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Aitken-Christie, J. Automation. In: Debergh, P.; Zimmerman, R. H., eds. Micropropagation: technology and application, Dordrecht: Kluwer Academic Publishers. 1991:363–388.
Capellades, M. Q. Histological and ecophysiological study of the changes occurring during the acclimatization ofin vitro cultures. Gent, Belgium:State University; 1990. Thesis.
Dejardins, Y.; Laforge, F.; Lussier, C., et al. Effect of CO2 enrichment and high photosynthetic photon flux on the development of autotrophy and growth of tissue-cultured strawberry, raspberry and asparagus plants. Acta Hortic. 230:47–57; 1988.
Doi, M.; Oda, H.; Asahira, T. In vitro atmosphere of cultured C3 and CAM plants in relation to day-length. Environ. Control Biol. 27:9–13; 1989. (In Japanese with English summary and captions).
Fujiwara, K.; Kozai, T.; Watanabe, I. Measurements of carbon dioxide gas concentration in closed vessels containing tissue cultured plant-lets and estimates of net photosynthetic rates of the plantlets. J. Agric. Meteorol. Japan, 43:21–30; 1987. (In Japanese with English summary and captions.)
Fujiwara, K.; Kozai, T.; Watanabe, I. Development of a photoautotrophic tissue culture system for shoots and/or plantlets at rooting and acclimatization stages. Acta Hort. 230:153–158; 1988.
Jan Rowe, W. New technologies in plant tissue culture. In: Zimmerman, R. H.; Griesbach, R. J.; Hammerschlag, F. A., et al., eds. Tissue culture as a plant production system for horticultural crops. Martinus Nijhoff Publishers: 1986:35–51.
Kozai, T.; Oki, H.; Fujiwara, K. Effects of CO2 enrichment and sucrose concentration under high photosynthetic photon fluxes on growth of tissue-culturedCymbidium plantlets during the preparation stage. In: Ducate, G.; Jacobs, M.; Simpson, A., eds. Plant micropropagation in horticultural industries. Belgium: Arlon; University Press, Liege; 1987:135–141.
Kozai, T.; Iwanami, Y.; Fujiwara, K. Effects of CO2 enrichment on the plantlet growth during the multiplication stage. Plant Tissue Cult. Lett. 4:22–26; 1987. (In Japanese with English summary and captions.)
Kozai, T. High technology in protected cultivation. Horticulture in new era. Tokyo: Organizing Committee of International Symposium on High Technology in Protected Cultivation. 1988:1–49.
Kozai, T.; Koyama, Y.; Watanabe, I. Multiplication of potato plantletsin vitro with sugar free medium under high photosynthetic photon flux. Acta Hortic. 230:121–127; 1988.
Kozai, T.; Kubota, C.; Watanabe, I. Effect of basal medium composition on the growth of carnation plantlets in auto- and mixotrophic tissue culture. Acta Hortic. 230:159–166; 1988.
Kozai, T.; Iwanami, Y. Effects of CO2 enrichment and sucrose concentration under high photon fluxes on plant growth of carnation in tissue culture during the preparation stage. J. Jpn. Soc. Hortic. Sci. 57:279–288; 1988.
Kozai, T.; Sekimoto, K. Effects of the number of air changes per hour of the stoppered vessel and the photosynthetic photon flux on the carbon dioxide concentration inside the vessel and the growth of strawberry plantletsin vitro. Environ. Control Biol. 26:21–29; 1988. (In Japanese with English summary and captions.)
Kozai, T.; Iwabuchi, K.; Watanabe, I. Effects of low dark period temperatures and CO2 enrichment during the photoperiod on the growth of strawberry plantletsin vitro (1) and (2). Abstracts of Annual Autumn Meet. Jpn. Soc. Hortic. Sci. 1988:270–273. (In Japanese.)
Kozai, T.; Nishiuchi, N.; Fujiwara, K., et al. Effects of planting density in the vessel on the growth and net photosynthetic rates of potato plantlets in photoautotrophic micropropagation. J. Jpn. Soc. Hortic. Sci. 58:(Special issue 1):244–255; 1989. (In Japanese with English captions.)
Kozai, T.; Kubota, C.; Watanabe, I. The growth of carnation plantletsin vitro cultured photoautotrophically and photomixotrophically on different media. Environ. Control Biol. 28:21–27; 1990. (In Japanese with English summary and captions.)
Kozai, T.; Takazawa, A.; Watanabe, I., et al. Growth of tobacco seedlings and plantletsin vitro as affected by in vitro environment. Environ. Control Biol. 28:31–39; 1990. (In Japanese with English summary and captions.)
Kozai, T.; Oki, H.; Fujiwara, K. Photosynthetic characteristics ofCymbidium plantletsin vitro. Plant Cell Tissue Organ Cult. 22:205–211; 1990.
Kozai, T.; Lee, H.; Hayashi, M. Photoautotrophic micropropagation ofRosa plantlets under CO2 enriched and high photosynthetic photon flux conditions. Italy: Abstracts of 23rd International Horticultural Congress. 1. Oral. International Society for Horticultural Science, Wageningen; 1990:173.
Kozai, T. Autotrophic (sugar free) tissue culture for promoting the growth of plantletsin vitro and for reducing biological contamination. Proc. Bangkok. Thailand: International Symposium on Application of Biotechnology for Small Industries; 1990:39–51.
Kozai, T. Micropropagation under photoautotrophic conditions. In: Debergh, P.; Zimmerman, R. H., eds. Micropropagation: technology and application. Dordrecht: Kluwer Academic Publishers. 1990:449–471.
Kozai, T.; Ohde, N.; Kubota, C. Similarity of growth patterns between plantlets and seedlings ofBrassica campestris L. under different in vitro conditions. Plant Cell Tissue Organ Cult. 24:187–191; 1991.
Kozai, T.; Iwabuchi, K.; Watanabe, K., et al. Photoautotrophic and photomixotrophic growth of strawberry plantletsin vitro and changes in nutrient composition of the medium. Plant Cell Tissue Organ Cult. In press; 1991.
Kozai, T. Autotrophic micropropagation. In: Bajaj, P. S., ed. Biotechnology in agriculture and forestry. Vol. 17 High-tech and micropropagation I. New York: Springer-Verlag. In press; 1991.
Kozai, T. Controlled environments in conventional and automated micropropagation. In: Levin, R.; Vasil, K., eds. Cell culture and somatic cell genetics of plants. Vol. 8. Scale-up and automation in plant tissue culture. London: Academic Press, Inc.; 1991:213–230.
Kozai, T. Environmental control and automation in micropropagation. In: Karube, I., ed. Automation in biotechnology. Amsterdam: Elsevier Science Publishers. In press; 1991.
Kuobta, C.; Kozai, T. Effects of forced ventilation on photoautotrophic growth of plug plantletsin vitro. Amsterdam: Abstracts of 7th International Congress on Plant Tissue and Cell Culture. International Society for Horticultural Science, Wageningen; 1990:114.
Mousseau, M. CO2 enrichmentin vitro. Effect on autotrophic and heterotrophic culture ofNicotiana tabacum (var. Samsun). Photosynth. Res. 8:187–191; 1986.
Murashige, T.; Skoog, F. A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol. Plant. 15:473–497; 1962.
Nakayama, M.; Kozai, T.; Watanabe, I. Effect of sugar concentration in the medium and ventilation condition on the net photosynthetic rate of potato explantsin vitro. Proceedings of 11th meeting of Plant Tissue Culture Society. Okayama. Japan: 1989:186–187.
Read, P. E. Environmental effects in micropropagation. In: Ammirato, P. V.; Evans, D. A.; Sharp, W. R., et al., eds. Handbook of plant cell culture, vol. 5. New York: McGraw-Hill Publishing Co.; 1989:95–125.
Shimada, N.; Tanaka, F.; Kozai, T. Effects of low O2 concentration on net photosynthesis of C3 plantletsin vitro. Acta Hortic. 230:171–175; 1988.
Tanaka, F.; Watanabe, Y.; Shimada, N. Effect of O2 concentration on photorespiration inChrysanthemum morifolium plantlets in plant tissue culture. Plant Tissue Cult. Lett. 7:85–91; 1990. (In Japanese with English summary and captions.)
Walker, P. N.; Heuser, C. W.; Heinemann, P. H. Micropropagation: studies of gaseous environments. Acta Hortic. 230:145–152; 1988.
Watanabe, K.; Watanabe, Y.; Shimada, N. Effect of sucrose concentration in the medium on growth, apparent photosynthesis and rebulose-1, 5-bisphosphate carboxylase ofSpathiphyllum plantlets in aeration culture. Plant Tissue Cult. Lett. 7:74–79; 1990. (In Japanese with English summary and captions.)
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Kozai, T. Photoautotrophic micropropagation. In Vitro Cell Dev Biol - Plant 27, 47–51 (1991). https://doi.org/10.1007/BF02632127
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DOI: https://doi.org/10.1007/BF02632127