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Control of cellular proliferation in human diploid fibroblasts

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Summary

Confluent monolayers of human diploid fibroblasts can be stimulated to synthesize DNA and divide by a change of medium. In the lag period between the application of the stimulus and the onset of DNA synthesis, there occurs a series of biochemical events which include the very early activation of the cell genome. Gene activation requires protein synthesis but not previous RNA synthesis. Experiments suggest that gene activation is dependent upon specific acidic nuclear proteins, whose synthesis is regulated at the post-transcriptional level.

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Authors and Affiliations

  1. Department of Pathology and Fels Research Institute, Temple University School of Medicine, 19140, Philadelphia, Pennsylvania

    Renato Baserga, Giovanni Rovera & John Farber

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  1. Renato Baserga
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  2. Giovanni Rovera
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  3. John Farber
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Additional information

This work was supported by research grants CA-08373 from the National Cancer Institute and DRF-1019 from the Damon Runyon Fund.

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Baserga, R., Rovera, G. & Farber, J. Control of cellular proliferation in human diploid fibroblasts. In Vitro 7, 80–87 (1971). https://doi.org/10.1007/BF02628266

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