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Effects of diabetes and vitamin D-depletion on duodenal and ileal calcium transport in the rat

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Summary

We compared effects of vitamin D depletion and alloxan-induced diabetes on intestinal calcium transport in growing rats. Transport of45Ca was studied using everted sacs of duodenum and ileum. Diabetic rats were compared with matched controls (diabetes studies); vitamin D-depleted animals were compared with depleted animals repleted with vitamin D 48 hrs prior to study (vitamin D studies). Duodenal calcium transport was reduced by half or less in experimental groups (diabetic or vitamin D-depleted rats) when compared with corresponding controls (control rats matched with diabetics or vitamin D-repleted animals matched with depleted animals):a) for serosal-to-mucosal (S/M) concentration ratios; andb) per unit weight of mucosa as net transport into serosal medium or net disappearance from mucosal medium. Based on transport into the serosal medium per unit of mucosa, ileal calcium transport was decreased in the vitamin D-depleted group compared with the vitamin D-repleted group in vitamin D studies. In contrast, in diabetes studies ileal calcium transport into the serosal medium was the same in diabetic and control animals. The finding that diabetes and vitamin D depletion have the same effects on duodenal calcium transport agrees witha) lack of specific, vitamin D-dependent duodenal calcium-binding protein in diabetes, andb) restoration of duodenal calcium transport in diabetics by either 1.25-dihydroxycholecalciferol or 1α-hydroxycholecalciferol. Depression of ileal calcium transport by vitamin D-depletion but not by diabetes shows the specificity of intestinal site with respect to mechanisms and control of calcium transport. Diabetes complements vitamin D depletion as a means for studying intestinal calcium transport.

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References

  1. Bray G. A.: A Simple Efficient Liquid Scintillator for Counting Aqueous Solutions in a Liquid Scintillation Counter — Ann. Biochem.1, 279, 1960.

    Article  CAS  Google Scholar 

  2. Dowdle E. B., Schachter D., Schenker H.: Requirement for Vitamin D for the Active Transport of Calcium by the Intestine — Amer. J. Physiol.198, 269, 1960.

    PubMed  CAS  Google Scholar 

  3. Drescher D., DeLuca H. F.: Vitamin D Stimulated Calcium Binding Protein from Rat Intestinal Mucosa. Purification and Some Properties — Biochemistry (Wash.)10, 2302, 1971.

    CAS  Google Scholar 

  4. Fiske C. H., Subbarow Y.: The Colorimetric Determination of Phosphorus — J. biol. Chem.66, 375, 1925.

    CAS  Google Scholar 

  5. Harrison H. E., Harrison H. C.: Transfer of Ca45 across Intestinal Wallin Vitro in Relation to Action of Vitamin D and Cortisol — Amer. J. Physiol.199, 265, 1960.

    PubMed  CAS  Google Scholar 

  6. Kimberg D. V., Schachter D., Schenker H.: Active Transport of Calcium by Intestine: Effects of Dietary Calcium — Amer. J. Physiol.200, 1256, 1961.

    PubMed  CAS  Google Scholar 

  7. Schachter D., Dowdle E. B., Schenker H.: Active Transport of Calcium by the Small Intestine of the Rat — Amer. J. Physiol.198, 263, 1960.

    PubMed  CAS  Google Scholar 

  8. Schachter D., Kimberg D. V., Schenker H.: Active Transport of Calcium by Intestine: Action and Bio-Assay of Vitamin D — Amer. J. Physiol.200, 1263, 1961.

    PubMed  CAS  Google Scholar 

  9. Schachter D., Rosen S. M.: Active Transport of Ca45 by the Small Intestine and its Dependence on Vitamin D — Amer. J. Physiol.196, 357, 1959.

    PubMed  CAS  Google Scholar 

  10. Schedl H. P., Wilson H. D.: Effects of Diabetes on Intestinal Growth in the Rat — J. exp. Zool.176, 487, 1971.

    Article  PubMed  CAS  Google Scholar 

  11. Schneider L. E., Omdahl J., Schedl H. P.: Evidence for Abnormal Vitamin D Metabolism in the Diabetic Rat — Physiologist18, 381, 1975.

    Google Scholar 

  12. Schneider L. E., Schedl H. P.: Diabetes and Intestinal Calcium Absorption in the Rat — Amer. J. Physiol.223, 1319, 1972.

    PubMed  CAS  Google Scholar 

  13. Schneider L. E., Schedl H. P.: Effects of Insulin on Duodenal Calcium Absorption in Streptozotocin Diabetic Rats — Clin. Res.23, 483, 1975.

    Google Scholar 

  14. Schneider L. E., Wasserman R. H., Schedl H. P.: Depressed Duodenal Calcium Absorption in the Diabetic Rat: Restoration bySolanum malacoxylon — Endocrinology97, 649, 1975.

    Article  PubMed  CAS  Google Scholar 

  15. Schneider L. E., Wilson H. D., Schedl H. P.: Intestinal Calcium Binding Protein in the Diabetic Rat — Nature (Lond.)245, 327, 1973.

    Article  CAS  Google Scholar 

  16. Schneider L. E., Wilson H. D., Schedl H. P.: Effects of Alloxan Diabetes on Duodenal Calcium Binding Protein in the Rat — Amer. J. Physiol.227, 832, 1974.

    PubMed  CAS  Google Scholar 

  17. Snedecor G. W., Cochran W. G.: Statistical Methods — Iowa State University Press, Ames, 1967; p. 555.

    Google Scholar 

  18. Somogyi M.: Notes on Sugar Determination — J. biol. Chem.195, 19, 1951.

    Google Scholar 

  19. Urban E., Schedl H. P.: Comparison ofin Vivo andin Vitro Effects of Vitamin D on Calcium Transport in the Rat — Amer. J. Physiol.217, 126, 1969.

    PubMed  CAS  Google Scholar 

  20. Urban E., Schedl H. P.: Vitamin D, Tissue Calcium, and Calcium Transport in thein Vivo Rat Small Intestine — Amer. J. Physiol.219, 944, 1970.

    PubMed  CAS  Google Scholar 

  21. Walker B., Schedl H. P.: Mechanism of the Reduced Calcium Transport in the Streptozotocin Diabetic Rat — Clin. Res.22, 636A, 1974.

  22. Wasserman R. H.: Calcium Absorption and Calcium-Binding Protein Synthesis:Solanum malacoxylon Reverses Strontium Inhibition — Science183, 1092, 1973.

    Article  Google Scholar 

  23. Wasserman R. H., Kallfelz F. A., Comar C. L.: Active Transport of Calcium by Rat Duodenumin Vivo — Science133, 883, 1961.

    Article  PubMed  CAS  Google Scholar 

  24. Wilson T. H., Wiseman G.: The Use of Sacs of Everted Small Intestine for the Study of the Transference of Substances from the Mucosal to the Serosal Surface — J. Physiol. (Lond.)123, 116, 1954.

    CAS  Google Scholar 

  25. Younoszai M. K., Schedl H. P.: Intestinal Calcium Transport: Comparison of Duodenum and Ileumin Vivo in the Rat — Gastroenterology62, 565, 1972.

    PubMed  CAS  Google Scholar 

  26. Younoszai M. K., Urban E., Schedl H. P.: Vitamin D and Intestinal Calcium Fluxesin Vivo in the Rat — Amer. J. Physiol.225, 287, 1973.

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Gastroenterology Research Laboratories Department of Medicine, University of Iowa College of Medicine, 52242, Iowa City, Ia, USA

    Louis E. Schneider, Helen D. Wilson & Harold P. Schedl

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  1. Louis E. Schneider
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  2. Helen D. Wilson
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  3. Harold P. Schedl
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Supported in part by Research Grant 16841 from the National Institute of Arthritis, Metabolic and Digestive Diseases, National Institutes of Health.

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Schneider, L.E., Wilson, H.D. & Schedl, H.P. Effects of diabetes and vitamin D-depletion on duodenal and ileal calcium transport in the rat. Acta diabet. lat 14, 18–25 (1977). https://doi.org/10.1007/BF02624660

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  • DOI: https://doi.org/10.1007/BF02624660

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