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Frontiers in mammalian cells culture

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Summary

For the past 60 years, fundamental discoveries in eukaryotic biology using mammalian cell cultures have been significant but modest relative to the enormous potential. Combined with advances in technologies of cell and molecular biology, mammalian cell culture technology is becoming a major, if not essential tool, for fundamental discovery in eukaryotic biology. Reconstruction of the milieu for cells has progressed from simple salt solutions supporting brief survival of tissues outside the body to synthesis of the complete set of structurally defined nutrients, hormones and elements of the extracellular matrix needed to reconstruct complex tissues from cells. The isolation of specific cell types in completely defined environments reveals the true complexity of the mammalian cell and its environment as a dynamic interactive physiological unit. Cell cultures provide the tool for detection and dissection of the mechanism of action of cellular regulators and the genes that determine individual aspects of cell behavior. The technology underpins advances in virology, somatic cell genetics, endocrinology, carcinogenesis, toxicology, pharmacology, hematopoiesis and immunology, and is becoming a major tool in develomental biology, complex tissue physiology and production of unique mammalian cell-derived biologicals in industry.

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Authors and Affiliations

  1. W. Alton Jones Cell Science Center, Inc., 10 Old Barn Road, 12946, Lake Placid, New York

    Wallace L. Mc Keehan & Gordon H. Sato

  2. Department of Biochemistry and Biophysics, Environmental Health Sciences Center, Oregon State University, 97331, Corvallis, Oregon

    David Barnes

  3. Departments of Molecular Pharmacology, Microbiology and Immunology, Albert Einstein College of Medicine, 10461, Bronx, New York

    Lola Reid

  4. Department of Microbiology and Molecular Genetics, University of California, Irvine, 92727, Irvine, California

    Eric Stanbridge

  5. Graduate School of Genetic Resources Technology, Kyushu University, 812, Fukuoka, Japan

    Hiroki Murakami

Authors
  1. Wallace L. Mc Keehan
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  2. David Barnes
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  3. Lola Reid
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  4. Eric Stanbridge
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  5. Hiroki Murakami
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  6. Gordon H. Sato
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This article is the first of a series of invited reviews aimed at identifying fundamental contributions and current challenges associated with research activities in subdiscriplines of cell and developmental biology in vitro. This treatise is dedicated to Dr. Brian Kimes, Program Director at the National Cancer Institute, whose vision, encouragement and support have contributed significantly to modern developments in mammalian cell culture.

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Keehan, W.L.M., Barnes, D., Reid, L. et al. Frontiers in mammalian cells culture. In Vitro Cell Dev Biol 26, 9–23 (1990). https://doi.org/10.1007/BF02624149

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  • DOI: https://doi.org/10.1007/BF02624149

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