Summary
In an attempt to understand events involved in the cellular regulation of in vitro plant organogenesis, experiments were performed in which tobacco (Nicotiana tabacum L.) callus was transferred at different days in culture from a shoot-forming medium to a non-shoot-forming medium and vice versa. The transfers were made at key histologic stages of the shoot-forming process and known biochemical and biophysical correlates were examined. The changes in starch accumulation and disappearance supported the previously assigned functions, and could be correlated with the histologic changes that occurred in the callus after transfer at the different culture times. In contrast, the changes in respiration could not be correlated with these events. The changes in osmotic and turgor potentials after transfer showed that osmotic adjustment preceded both shoot initiation and development. This suggests that osmotic adjustment might play an important role in in vitro organogenesis.
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References
Brown, D. C. W.; Leung, D. W. M.; Thorpe, T. A. Osmotic requirement for shoot formation in tobacco callus. Physiol. 46:36–41; 1979.
Brown, D. C. W.; Thorpe, T. A. Changes in water potential and its components during shoot formation in tobacco callus. Physiol. Plant. 48:83–87; 1980.
Brown, D. C. W.; Thorpe, T. A. Adenosine phosphate and nicotinamide adenine dinucleotide pool sizes during shoot initiation in tobacco callus. Plant Physiol. 65:587–590; 1980.
Cleland, R. E. The control of cell enlargement. In: Jennings, D. H., ed. Integration of activity in the higher plant. Cambridge: University Press; 1977:101–115.
Christianson, M. L.; Warnick, D. A. Phenocritical times in the process of in vitro shoot organogenesis. Dev. Biol. 101:382–390; 1984.
Christianson, M. L.; Warnick, D. A. Temporal requirement for phytohormone balance in the control of organogenesis in vitro. Dev. Biol. 112:494–497; 1985.
Jensen, W. A. Botanical histochemistry, principles and practice. San Francisco: W. H. Freeman & Co.; 1962.
Karnovsky, M. J. A formaldehyde-glutaraldehyde fixative of high osmolarity for use in electron microscopy. J. Cell Biol. 27:137A-138A; 1965.
Kishor, P. B. K.; Reddy, G. M. Regeneration of plants from long-term cultures ofOryza sativa L. Plant Cell Rep. 5:391–393; 1986.
Levenson, R.; Housman, D. Commitment: How do cells make the decision to differentiate? Cell 25:5–6; 1981.
Maeda, E.; Thorpe, T. A. Shoot histogenesis in tobacco callus cultures. In Vitro 15:415–424; 1979.
Murashige, T.; Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissuecultures. Physiol. Plant. 11:473–497; 1962.
O'Brien, T. P.; McCully, M. E. The study of plant structure, principles and selected methods. Melbourne, Australia: Termacarphi Pty. Ltd.; 1981.
Ross, M. K.; Thorpe, T. A. Physiological gradients and shoot initiation in tobacco callus. Plant Cell Physiol. 14:473–480; 1973.
Sass, J. E. Botanical microtechnique, 3rd ed. Ames: Iowa State University Press; 1958.
Thorpe, T. A. Carbohydrate availability and shoot formation in tobacco callus cultures. Physiol. Plant. 30:77–81; 1974.
Thorpe, T. A. Organogenesis in vitro: Structural, physiological and biochemical aspects. Int. Rev. Cytol. Suppl 11A:71–112; 1980.
Thorpe, T. A. Morphogenesis and regeneration in tissue culture. In: Owens, L. D., ed. Genetic engineering: Application to agriculture. Totowa, NJ; Rowman and Allanheld; 1983:285–303.
Thorpe, T. A.; Beaudoin-Eagan, L. D.14C-metabolism during growth and shoot formation in tobacco callus. Zeit. Pflanzenphysiol. 113:337–346; 1984.
Thorpe, T. A.; Laishley, E. J. Glucose oxidation during shoot initiation in tobacco callus cultures. J. Exp. Bot. 24:1082–1089; 1973.
Thorpe, T. A.; Meier, D. D. Starch metabolism, respiration and shoot formation in tobacco callus cultures. Physiol. Plant. 27:365–369; 1972.
Thorpe, T. A.; Meier, D. D. Effect of gibberellic acid on starch metabolism in tobacco callus cultured under shoot-forming conditions. Phytomorphology 25:238–245; 1975.
Thorpe, T. A.; Murashige, T. Some histochemical changes underlying shoot initiation in tobacco callus cultures. Can. J. Bot. 28:277–285; 1970.
White, P. R. Nutrient deficiency studies and an improved inorganic nutrient for cultivation of excised tomato roots. Growth 7:53–65; 1943.
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This research was supported by the Natural Sciences and Engineering Research Council of Canada grant A-6467 to T. A. T.
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Hammersley-Straw, D.R.H., Thorpe, T.A. Modulation of shoot formation in tobacco callus by tissue transfer between permissive and inhibitory media. In Vitro Cell Dev Biol 23, 867–872 (1987). https://doi.org/10.1007/BF02620967
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DOI: https://doi.org/10.1007/BF02620967