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Ecdysone action on insect cell lines

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Summary

Cell lines derived from embryos of the tobacco hornworm,Manduca sexta (L.), showed a marked morphological response to treatment with physiological doses of β-ecdysone. The response of these cell lines with α-ecdysone indicated that the penta-ol (α-ecdysone) must be converted to the hexa-ol (β-ecdysone) form before the morphological response can appear. Liquid chromatographic analysis of the spent medium confirmed that the cells converted the α-ecdysone to β-ecdysone in amounts that exceeded the threshold level for a biological response. Constant exposure to β-ecdysone produced a cell line that was resistant to the killing effect of β-ecdysone and failed entirely to respond to the presence of α-ecdysone.

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References

  1. Courgeon, A. M. 1972. Action of insect hormones at the cellular level. Exp. Cell Res. 74: 327–336.

    Article  PubMed  CAS  Google Scholar 

  2. Echalier, G., and A. Ohanessian. 1969. Isolement en cultures in vitro de lignes cellulaire diploides deDrosophila melanogaster. C. R. Acad. Sci. 268: 1771–1773.

    CAS  Google Scholar 

  3. Mitsuhashi, J., and T. D. C. Grace. 1970. The effects of insect hormones on the multiplication rates of cultured insect cells in vitro. Appl. Entomol. Zool. 5: 182–188.

    CAS  Google Scholar 

  4. Reinecke, J. P., and J. Robbins. 1971. Reaction of an insect cell line to ecdysterone. Exp. Cell Res. 64: 335–338.

    Article  PubMed  CAS  Google Scholar 

  5. Cohen, E., and L. Gilbert. 1972. Metabolic and hormonal studies on two insect cell lines. J. Insect Physiol. 18: 1061–1076.

    Article  PubMed  CAS  Google Scholar 

  6. Cohen, E., N. Lanir, and E. Englander. 1976. Morphological effects and metabolism of the molting hormone inAedes aegypti. Insect Biochem. 6: 433–439.

    Article  CAS  Google Scholar 

  7. Wyss, C. 1976. Juvenile hormone analogue counteracts growth stimulation and inhibition by ecdysones in clonalDrosophila cell line. Experientia 32: 1272.

    Article  PubMed  CAS  Google Scholar 

  8. Cherbas, P., L. Cherbas, and C. M. Williams. 1977. Induction of acetylcholinesterase activity by β-ecdysone in aDrosophila cell line. Science 197: 275–277.

    Article  PubMed  CAS  Google Scholar 

  9. Eide, P. E., J. Caldwell, and E. Marks. 1975. Establishment of two cell lines from embryonic tissue of the tobacco hornworm,Manduca sexta (L.). In Vitro 6: 395–399.

    Article  Google Scholar 

  10. Yunker, C., J. Vaughn, and J. Corey. 1967. Adaptation of a cell line (Grace'sAntherea cells) to a medium free of insect hemolymph. Science 155: 1565–1566.

    Article  PubMed  CAS  Google Scholar 

  11. Holman, G., and R. Meola. HPLC method for the purification and analysis of insect ecdysones: Application to measurement of ecdysone titers during pupal-adult development of an insect. Insect Biochem., in press.

  12. Marks, E. P. 1976. Metabolism of molting hormone analogs by cultured cockroach tissues. In: E. Kurstak, and K. Maramorosch (Eds.),Invertebrate Tissue Culture. Academic Press, New York, pp. 223–226.

    Google Scholar 

  13. Furlenmeier, A., A. Furst, A. Langemann, G. Waldvogel, P. Hocks, U. Kerb, and R. Weichert. 1967. Zur Syntheses des Ecdysones IX Mitt. Über Insektenhormone. Helv. Chim. Acta 50: 2387–2396.

    Article  PubMed  CAS  Google Scholar 

  14. Marks, E. P. 1973. Deposition of insect cuticle in vitro: Differential responses to α- and β-ecdysones. Gen. Comp. Endocrinol. 21: 472–477.

    Article  PubMed  CAS  Google Scholar 

  15. King, D., and E. Marks. 1974. Secretion and metabolism of α-ecdysone by cockroach (Leucophaea maderae) tissues in vitro. Life Sci. 15: 147–154.

    Article  PubMed  CAS  Google Scholar 

  16. King, D. 1972. Metabolism of α-ecdysone and possible precursors in insects in vivo and in vitro. Gen. Comp. Endocrinol. 3 (Suppl.): 221–227.

    Article  Google Scholar 

  17. Oberlander, H. 1976. Hormonal control of growth and differentiation of insect tissues cultured in vitro. In Vitro 12: 225–235.

    PubMed  CAS  Google Scholar 

  18. Schneider, I. 1972. Cell lines derived from late embryonic stages ofDrosophila melanogaster. J. Embryol. Exp. Morphol. 27: 352–365.

    Google Scholar 

  19. Landureau, J., and P. Grellet. 1975. Obtention de lignees permanentes d'hemocytes de blatte. Characteristiques physiologiques et ultrastructurales. J. Insect Physiol. 21: 137–151.

    Article  Google Scholar 

  20. Shannon, J. E. 1977. The cell source information bank (CSIB) referral service. In Vitro 13: 335.

    Google Scholar 

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Author information

Authors and Affiliations

  1. Metabolism and Radiation Research Laboratory, Agricultural Research Science and Education Administration, U.S. Department of Agriculture, 58102, Fargo, North Dakota

    Edwin P. Marks

  2. Veterinary, Toxicology, and Entomology Research Laboratory, Agricultural Research Science and Education Administration, U.S. Department of Agriculture, 77840, College Station, Texas

    G. Mark Holman

Authors
  1. Edwin P. Marks
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  2. G. Mark Holman
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Additional information

Most of the experimental work for this study was done at the Biological Laboratories of Harvard University while the senior author was on sabbatical leave from the Metabolism and Radiation Research Laboratory. The research employed facilities supported by grants made by NIH and NSF to Professor C. M. Williams.

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Marks, E.P., Holman, G.M. Ecdysone action on insect cell lines. In Vitro 15, 300–307 (1979). https://doi.org/10.1007/BF02618955

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