Skip to main content
Log in

Two monotreme cell lines, derived from female platypuses (Ornithorhynchus anatinus; Monotremata, mammalia)

  • Published:
In Vitro Aims and scope Submit manuscript

Summary

Two diploid platypus cell lines, designated Oa-1F and Oa-2F, have been derived from the toe webs of two females. The development and growth characteristics of the lines are described and G-banded karyotypes presented (the first reported for the platypus). The availability of these lines will greatly facilitate chromosome and gene mapping studies of the platypus and permit the extension of comparative studies of mammalian chromosome and genome evolution to the monotremes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Griffiths, M. The biology of the monotremes. New York: Academic Press; 1978.

    Google Scholar 

  2. Crompton, A. W.; Jenkins, F. A., Jr. Origin of mammals. Lillegraven, J. A.; Kielan-Jaworowska, Z.; Clemens, W. A. eds. Mesozoic mammals. Berkeley, CA: University of California Press; 1979: 59–73.

    Google Scholar 

  3. Kemp, T. S. Mammal-like reptiles and the origin of mammals. New York: Academic Press; 1982.

    Google Scholar 

  4. Nash, W. G.; O'Brien, S. J. Conserved regions of homologous G-banded chromosomes between orders in mammalian evolution: carnivores and primates. Proc. Natl. Acad. Sci. USA. 79: 6631–6635; 1982.

    Article  PubMed  CAS  Google Scholar 

  5. Pearson, P. L.; Roderick, T. H.; Davisson, M. T.; Lalley, P. A.; O'Brien, S. J. Report of the committee on Comparative Mapping. 6th International Workshop on Human Gene Mapping, Oslo (1981). Cytogenet. Cell Genet. 32: 208–220; 1982.

    PubMed  CAS  Google Scholar 

  6. Ohno, S. Evolution of sex chromosomes in mammals. Ann. Rev. Genet. 3: 495–524; 1969.

    Article  Google Scholar 

  7. Cooper, D. W.; Johnston, P. G.; Murtagh, C. E.; Sharman, G. B.; Poole, W. E. Sex-linked isozymes and chromosome evolution and inactivation in kangaroos. Markert, C. L. ed. Isozymes III. Developmental Biology. New York: Academic Press; 1975: 559–573.

    Google Scholar 

  8. Graves, J. A. M.; Chew, G. K.; Cooper, D. W.; Johnston, P. G. Marsupial-mouse cell hybrids containing fragments of the marsupial X chromosome. Somat. Cell Genet. 5: 481–489; 1979.

    Article  PubMed  CAS  Google Scholar 

  9. Donald, J. A.; Hope, R. M. Mapping a marsupial X chromosome using kangaroo-mouse somatic cell hybrids. Cytogenet. Cell Genet. 29: 127–137; 1981.

    PubMed  CAS  Google Scholar 

  10. Pye, D.; MacGregor, A.; Stanley, J. F. Marsupial cells in long term culture. In Vitro 13: 232–236; 1977.

    Article  PubMed  CAS  Google Scholar 

  11. Dawson, T. R.; Grant, T. R.; Fanning, D. Standard metabolism of monotremes and the evolution of homeothermy. Aust. J. Zool. 27: 511–515; 1979.

    Article  Google Scholar 

  12. Seabright, M. A rapid banding technique for human chromosomes. Lancet (2): 971–972; 1971.

    Article  PubMed  CAS  Google Scholar 

  13. Eichenbaum, S. Z.; Krumins, E. J. A simple and reliable method of chromosome banding for prenatal cytogenetics using a bromodeoxyuridine pulse. Prenatal Diagnosis 3: 1–6; 1983.

    Article  Google Scholar 

  14. Leversha, M.; Sinfield, C.; Webb, G. Rapid and reliable methods for the G- and C-banding of human and other mammalian chromosomes. Aust. J. Med. Lab. Sci. 1: 139–143; 1980.

    Google Scholar 

  15. Rubin, H. A substance in conditioned medium which enhances the growth of small numbers of chick embryo cells. Exp. Cell Res. 41: 138–148; 1966.

    Article  PubMed  CAS  Google Scholar 

  16. Rein, A.; Rubin, H. Effects of local cell concentrations upon the growth of chick embryo cells in tissue culture. Exp. Cell Res. 49: 666–678; 1968.

    Article  PubMed  CAS  Google Scholar 

  17. Ferguson-Stanley, J.; Pye, D.; MacGregor, A. Comparison of doubling numbers attained by animal cells with life span of species. Nature 255: 158–159; 1975.

    Article  Google Scholar 

  18. Martin, G. M.; Sprague, C. A.; Epstein, C. J. Replicative life-span of cultivated human cells. Effects of donor's age, tissue and genotype. Lab. Invest. 23: 86–92; 1970.

    PubMed  CAS  Google Scholar 

  19. Graves, J. A. M.; Hope, R. M. Fusion and hybridization of marsupial and eutherian cells. I. Growth of marsupial cells. Aust. J. Biol. Sci. 30: 445–449; 1977.

    Google Scholar 

  20. Hayflick, L.; Moorhead, P. S. The serial cultivation of human diploid cell strains. Exp. Cell Res. 25: 585–621; 1961.

    Article  Google Scholar 

  21. Macieira-Coelho, A.; Azzarone, B. Aging of human fibroblasts is a succession of subtle changes in the cell cycle and has a final short stage with abrupt events. Exp. Cell Res. 141: 325–332; 1982.

    Article  PubMed  CAS  Google Scholar 

  22. Bick, Y. A. E.; Sharman, G. B. The chromosomes of the platypus (Ornithorhynchus: Monotremata): Cytobios 14: 17–25; 1975.

    Google Scholar 

  23. Murtagh, C. E. A unique cytogenetic system in monotremes. Chromosoma 65: 37–57; 1977.

    Article  Google Scholar 

  24. Pathak, S.; Stock, A. D. The X chromosomes of mammals: karyological homology as revealed by banding techniques. Genetics 78: 703–713; 1974.

    PubMed  CAS  Google Scholar 

  25. Rofe, R. G-banding and chromosomal evolution in Australian marsupials. Univ. of Adelaide, South Australia; 1979. Thesis.

    Google Scholar 

  26. Ohno, S. Sex chromosomes and sex-linked genes. Springer-Verlag, Berlin; 1967.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wrigley, J.M., Marshall Graves, J.A. Two monotreme cell lines, derived from female platypuses (Ornithorhynchus anatinus; Monotremata, mammalia). In Vitro 20, 321–328 (1984). https://doi.org/10.1007/BF02618595

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02618595

Key words

Navigation