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Isolation and characterization of diploid clones from adult and newborn rat liver cell lines

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Summary

A high frequency of diploid and near-diploid clones were developed from cell lines derived from adult and newborn rat liver using micropipettes. There were some differences in morphology, biochemical properties and growth rate between clones. Cloned cells had low levels of tyrosine transaminase activity, glucose-6-phosphatase activity and albumin content. A diploid clone and a pseudodiploid clone derived from adult rat liver cell line were positive for α-fetoprotein.

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References

  1. Bausher, J., and W. I. Schaffer. 1974. A diploid cell culture. 1. Characterization and sensitivity to Aflatoxin B1. In Vitro 9: 286–293.

    Article  CAS  Google Scholar 

  2. Borek, C. 1972. Neoplastic transformationin vitro of a clone of adult liver epithelial cells into differentiated hepatoma-like cells under conditions of nutritional stress. Proc. Nat. Acad. Sci. U.S.A. 69: 956–959.

    Article  CAS  Google Scholar 

  3. Borenfreund, E., P. J. Higgins, M. Steinglass, and A. Bendich. 1975. Properties and malignant transformation of established rat liver parenchymal cells in culture. J. Nat. Cancer Inst. 55: 375–384.

    PubMed  CAS  Google Scholar 

  4. Coon, H. G. 1968. Clonal culture of differentiated rat liver cells (abstr.). J. Cell Biol. 39: 29.

    Google Scholar 

  5. Diamond, L., R. McFall, Y. Tashiro, and D. Sabatini. 1973. The WIRL-3 rat liver cell lines and their transformed derivatives. Cancer Res. 33: 2627–2636.

    PubMed  CAS  Google Scholar 

  6. Gerschenson, L. E., M. Anderson, J. Molson, and T. Okigaki. 1970. Tyrosine transaminase induction by dexamethasone in a new rat liver cell line. Science 179: 858–861.

    Google Scholar 

  7. Iype, P. T. 1971. Cultures from adult rat liver cells. I. Establishment of monolayer cell cultures from normal liver. J. Cell Physiol. 79: 281–288.

    Article  Google Scholar 

  8. Katsuta, H., and T. Takaoka. 1963. Carcinogenesis in tissue culture I. Cultivation of normal rat liver cells. Jpn. J. Exp. Med. 33: 265–275.

    PubMed  CAS  Google Scholar 

  9. Masuji, H., H. Nakabayashi, and J. Sato. 1974. Long-term cultivation of two diploid epithelial cell lines derived from normal rat liver cells. Acta Med. Okayama 28: 281–293.

    PubMed  CAS  Google Scholar 

  10. Williams, G. M., and J. M. Gunn. 1974. Longterm cell culture of adult rat liver epithelial cells. Exp. Cell Res. 89: 139–142.

    Article  PubMed  CAS  Google Scholar 

  11. Weinstein, I. B., N. Yamazaki, J. M. Orenstein, R. Gebert, and M. E. Kaighn. 1975. Growth and structural properties of epithelial cell cultures established from normal rat liver and chemically induced hepatomas. Cancer Res. 35: 253–263.

    PubMed  CAS  Google Scholar 

  12. Grisham, J. W., S. B. Thal, and A. Nagel. 1975. Cellular derivation of continuously cultured epithelial cells from normal rat liver. In: L. E. Gerschenson, and E. B. Thompson (Eds.),Gene Expression and Carcinogenesis in Cultured Liver. Academic Press. London, pp. 1–23.

    Google Scholar 

  13. Cooper, J. E. K. 1970. Rapid isolation of single-cell clones of mammalian cell cultures. Tex. Rep. Biol. Med. 28: 29–35.

    PubMed  CAS  Google Scholar 

  14. Lwoff, A., R. Dulbecco, M. Vogt, and M. Lwoff. 1955. Kinetics of the release of poliomyelitis virus from single cells. Virology 1: 128–139.

    Article  PubMed  CAS  Google Scholar 

  15. Sanford, K. K., W. R. Earle, and G. D. Likely. 1948. The growth in vitro of single isolated tissue cells. 9: 229–246.

    CAS  Google Scholar 

  16. Tsutamune, T. 1975. Establishment of five cell lines from normal adult rat liversin vitro. Acta Med. Okayama 29: 137–145.

    PubMed  CAS  Google Scholar 

  17. Rothfels, K. H., and L. Siminovitch. 1958. An air-drying technique for flattening chromosomes in mammalian cells grownin vitro. Stain Technol. 33: 73–77.

    PubMed  CAS  Google Scholar 

  18. Seabright, M. 1971. A rapid banding technique for human chromosomes. Lancet 30: 971–972.

    Article  Google Scholar 

  19. Committee for a Standardized Karyotype ofRattus norvegicus. 1973. Standard karyotype of the Norway rat,Rattus norvegicus. Cytogenet. Cell Genet. 12: 199–205.

    Article  Google Scholar 

  20. Katsuta, H., T. Takaoka, and Y. Oishi. 1957. Further studies on the cultivation of fibroblasts in the simplified replicate tissue culture. Jpn. J. Exp. Med. 27: 19–29.

    PubMed  CAS  Google Scholar 

  21. Koide, H., and T. Oda. 1959. Pathological occurrence of glucose-6-phosphatase in serum in liver diseases. Clin. Chem. Acta 4: 554–561.

    Article  CAS  Google Scholar 

  22. Granner, D. K., and G. M. Tomkins. 1970. Tyrosine aminotransferase (rat liver). In: H. Tabor, and C. W. Tabor (Eds.),Methods in Enzymology. Vol. 17A. Academic Press, London, pp. 633–637.

    Google Scholar 

  23. Watanabe, A., O. Mori, K. Taketa, and K. Kosaka. 1975. Purification and immunochemical characterization of alpha-fetoprotein from rat fetal serum and liver. Acta Med. Okayama 29: 355–366.

    PubMed  CAS  Google Scholar 

  24. Miyazaki, M. 1978. Primary culture of adult rat liver cells. II. Cytological and biochemical properties of primary cultured cells. Acta Med. Okayama 32: 11–22.

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Division of Pathology, Cancer Institute, Okayama University Medical School, Shikata-cho 2-5-1, 700, Okayama, Japan

    Takayoshi Tokiwa, Hidekazu Nakabayashi, Masahiro Miyazaki & Jiro Sato

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  1. Takayoshi Tokiwa
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  2. Hidekazu Nakabayashi
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  3. Masahiro Miyazaki
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  4. Jiro Sato
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Additional information

This work was supported by a grant for cancer research from the Japanese Ministry of Education.

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Tokiwa, T., Nakabayashi, H., Miyazaki, M. et al. Isolation and characterization of diploid clones from adult and newborn rat liver cell lines. In Vitro 15, 393–400 (1979). https://doi.org/10.1007/BF02618406

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  • DOI: https://doi.org/10.1007/BF02618406

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