Summary
By using a chemically defined serum-free (SF) medium for propagation of Mm5mtc1 mouse adenocarcinoma cell cultures and clonal derivatives, medium components including hormones, glucose and individual amino acids were evaluated as to modulation of mouse mammary tumor virus (MMTV) production. Insulin, hydrocortisone and dexamethasen each increased MMTV production on a per cell basis over constitutive expression that ocures in SF medium devoid of hormones. Maximum production occurred when all three hormones were present. Hormone-stimulated virus expression also was influenced by glucose concentration. Cell growth and maximum MMTV expression increased when thyroxine, asparagine, proline and serine were omitted from the medium formulation. The resulting modified SF medium provides and ideal system for the propagation of high MMTV-producer clones and for the study of the biochemical regulation of MMTV expression.
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This work was supported in part by the Virus Cancer Program, Contract No. N01-CO-25423, National Cancer Institute, NIH, Bethesda, Maryland.
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Nagle, S.C., Fine, D.L. Demonstration of components of serum-free culture medium effecting maximum in vitro expression of mouse mammary tumor virus. In Vitro 14, 218–226 (1978). https://doi.org/10.1007/BF02618226
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DOI: https://doi.org/10.1007/BF02618226