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Characteristics of in vitro colony formation by cells from haemopoietic tissues

  • Part I Hemopoietic Precursors
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Summary

The characteristics of stimulation of colony formationin vitro from cells of mouse haemopoietic tissues has been briefly reviewed. Mouse kidney or embryo feeder cells, media conditioned by the cells from these tissues, normal or leukemic mouse sera, sera from leukemic or infectious mononucleosis patients, human urine and mouse embryo extracts are all sources of colony stimulating activity and their properties have been described.

All sources of colony-stimulating activity produce clones of cells of the granulocyte series. In tritiated thymidine treated mice injection of preparations rich in colony-stimulating activity has been shown to produce a neutrophil leucocytosis and accelerate the rate of accumulation of labelled neutrophils in the blood.

It is suggested that thein vitro assay can detect factors capable of stimulating granulocyte development.

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References

  1. Pluznik, D. H. and Sachs, L. 1965. The cloning of normal “mast” cells in tissue culture. J. Cell. Comp. Physiol.66: 319–324.

    Article  CAS  Google Scholar 

  2. Bradley, T. R. and Metcalf, D. 1966. The growth of mouse bone marrow cellsin vitro. Aust. J. Exp. Biol. & Med. Sci.44: 287–300.

    CAS  Google Scholar 

  3. Bradley, T. R., Robinson, W. and Metcalf, D. 1967. Colony productionin vitro by normal polycythaemic and anaemic bone marrow. Nature (London)213: 511.

    Article  Google Scholar 

  4. Robinson, W. A., Bradley, T. R. and Metcalf, D. 1967. Effect of whole body X-irradiation on colony production by bone marrow cellsin vitro. Proc. Soc. Exp. Biol. & Med.125: 388–391.

    CAS  Google Scholar 

  5. McCulloch, E. A. and Till, J. E. 1962. The sensitivity of cells from normal mouse bone marrow to gamma radiationin vitro andin vivo. Radiation Res.16: 822–829.

    Article  PubMed  CAS  Google Scholar 

  6. Till, J. E. and McCulloch, E. A. 1961. A direct measurement of the radiation sensitivity of normal mouse bone marrow cells. Radiation Res.14: 213–222.

    Article  PubMed  CAS  Google Scholar 

  7. Barnes, D. W. H. and Loutit, J. F. 1967. Haemopoietic stem cells in the peripheral blood. The Lancet (ii): 1138–1141.

    Article  Google Scholar 

  8. Wu, A. M., Siminovitch, L., Till, J. E. and McCulloch, E. A. 1968. Evidence for a relationship between mouse hemopoietic stem cells and cells forming colonies in culture. Proc. Nat. Acad. Sci.59: 1209–1215.

    Article  PubMed  CAS  Google Scholar 

  9. Foster, R. S., Jr., Bennett, M., Metcalf, D. and Cudkowicz, G. (Unpublished data.)

  10. Bradley, T. R. 1968. Aspects of stimulation of bone marrow colony growthin vitro. Aust. J. Exp. Biol. & Med. Sci. (In Press).

  11. Pluznik, D. H. and Sachs, L. 1966. The induction of clones of normal “mast” cells by a substance from conditioned medium. Exp. Cell Res.43: 553–563.

    Article  PubMed  CAS  Google Scholar 

  12. Bradley, T. R. and Sumner, M. A. 1968. Stimulation of mouse bone marrow colony growthin vitro by conditioned medium. Aust. J. Exp. Biol. Med. Sci. (In Press).

  13. Bradley, T. R., Metcalf, D. and Robinson, W. 1967. Stimulation by leukemic sera colony formation in solid agar cultures by proliferation of mouse bone marrow cells. Nature (London)213: 926–927.

    Article  CAS  Google Scholar 

  14. Eagle, H. 1959. Amino acid metabolism in mammalian cell cultures. Science130: 432–437.

    Article  PubMed  CAS  Google Scholar 

  15. Robinson, W. D., Metcalf, D. and Bradley, T. R. 1967. Stimulation by normal and leukemic mouse sera of colony formationin vitro by mouse bone marrow cells. J. Cell Physiol.69: 83–91.

    Article  Google Scholar 

  16. Metcalf, D. and Foster, R. 1967. Bone marrow colony-stimulating activity of serum from mice with viral-induced leukemia. J. Nat. Cancer Inst.39: 1235–1243.

    PubMed  CAS  Google Scholar 

  17. Foster, R., Jr., Metcalf, D. and Kirchinger, R. 1968. Induction of bone marrow colony-stimulating activity by a filterable agent in leukemic and normal mouse serum. J. Exp. Med. (In Press.)

  18. Metcalf, D., Foster, R. and Pollard, M. 1967. Colony stimulating activity of serum from germ-free normal and leukemic mice. J. Cell Physiol.70: 131–132.

    Article  PubMed  CAS  Google Scholar 

  19. Foster, R., Jr., Metcalf, D., Robinson, W. A., and Bradley, T. R. 1968. Bone marrow colony stimulating activity in human sera. Brit. J. Haematol. (In Press.)

  20. Dawe, C. J. and Potter, M. 1957. Morphological and biological progression of a lymphoid neoplasm of the mousein vivo andin vitro. Am. J. Pathol.33: 603.

    Google Scholar 

  21. Herzenberg, L. A. and Roosa, R. A. 1960. Nutritional requirements for growth of a mouse lymphoma in cell culture. Exp. Cell Res.21: 430–438.

    Article  PubMed  CAS  Google Scholar 

  22. Metcalf, D., Bradley, T. R. and Robinson, W. 1967. Analysis of colonies developingin vitro from mouse bone marrow cells stimulated by kidney feeder layers on leukemic serum. J. Cell. Physiol.69: 93–108.

    Article  Google Scholar 

  23. Ichikawa, Y., Pluznik, D. H. and Sachs, L. 1966.In vitro control of the development of macrophage and granulocyte colonies. Proc. Nat. Acad. Sci.56: 488–495.

    Article  PubMed  CAS  Google Scholar 

  24. Virolainen, M. and Defendi, V. 1967. Dependence of macrophage growthin vitro upon interaction with other cell types. Wistar Symposium Monograph No. 7,Growth Regulating Substances for Animal Cells in Culture, pp. 67–85.

  25. Senn, J. S., McCulloch, E. A. and Till, J. E. 1967. Comparison of colony forming ability of normal and leukemic human marrow in cell culture. The Lancet (ii): 597–598.

    Article  Google Scholar 

  26. Andrews, P. 1965. The gel-filtration behaviour of proteins related to their molecular weights over a wide range. Biochem. J.96: 595–606.

    PubMed  CAS  Google Scholar 

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Author notes

  1. T. R. Bradley & M. Sumner

    Present address: The Department of Physiology, University of Melbourne, Australia

  2. D. Metcalf & R. Stanley

    Present address: The Walter and Eliza Hall Institute for Medical Research, Parkville, Victoria, Australia

Authors and Affiliations

  1. The Department of Physiology, University of Melbourne, Australia

    T. R. Bradley, D. Metcalf, M. Sumner & R. Stanley

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  1. T. R. Bradley
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  2. D. Metcalf
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  3. M. Sumner
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  4. R. Stanley
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Bradley, T.R., Metcalf, D., Sumner, M. et al. Characteristics of in vitro colony formation by cells from haemopoietic tissues. In Vitro 4, 22–35 (1969). https://doi.org/10.1007/BF02618208

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