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Colony Formation: An Assay of Hematopoietic Progenitor Cells

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Stem Cell Mobilization

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2017))

Abstract

The colony-forming cell (CFC) assay is used to study the proliferation and differentiation pattern of each input hematopoietic progenitors by their ability to form colonies in a semisolid medium. The resulting colonies are consisting of more differentiated cells, and the number and the morphology of the colonies provide preliminary information about the ability of progenitors to differentiate and proliferate. To allow colonies to grow to a size which facilitates accurate counting and identification, about 14 days of culture is sufficient. In certain situations also shorter periods may be used.

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Authors and Affiliations

  1. Institute for Transfusion Medicine, German Red Cross Blood Donation Service North-East, Medical Faculty Carl Gustav Carus, Technical University Dresden, Dresden, Germany

    Romy Kronstein-Wiedemann & Torsten Tonn

Authors
  1. Romy Kronstein-Wiedemann
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  2. Torsten Tonn
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Corresponding author

Correspondence to Torsten Tonn .

Editor information

Editors and Affiliations

  1. Department of Internal Medicine II, Center for Medical Research, University of Tübingen, Tübingen, Germany

    Gerd Klein

  2. Institute of Transfusion Medicine and Immunology, Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany

    Patrick Wuchter

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Kronstein-Wiedemann, R., Tonn, T. (2019). Colony Formation: An Assay of Hematopoietic Progenitor Cells. In: Klein, G., Wuchter, P. (eds) Stem Cell Mobilization. Methods in Molecular Biology, vol 2017. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9574-5_3

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  • DOI: https://doi.org/10.1007/978-1-4939-9574-5_3

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9573-8

  • Online ISBN: 978-1-4939-9574-5

  • eBook Packages: Springer Protocols

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