Summary
Thirty-one compounds have been identified that act as cryoprotective agents for cultured mammalian cells. Eight compounds were comparable to dimethylsulfoxide (DMSO) in cryprotective effectivenes. Many of the cryoprotective compounds studied also (a) promote cell fusion and (b) induce cell differentiation in erythroleukemia and other cell systems. Thus previously unrecognized effects on the differentiated state of cells may occur when cells are treated with cryoprotective agents.
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References
Lovelock, J. E.; Bishop, M. W. H. Prevention of freezing damage to living cells by dimethyl sulphoxide. Nature 183: 1394–1395; 1959.
Hay, R. J. Preservation of cell-culture stocks in liquid nitrogen. TCA Manual 4: 787–790; 1978.
Farrant, J. Is there a common mechanism of protection of living cells by polyvinylpyrrolidone and glycerol during freezing? Nature 222: 1175–1176; 1969.
Persidsky, M. D.; Ellett, M. Hydroxyethyl starch as a cryoprotective agent for nucleated mammalian cells. Cryobiology 8: 586–588; 1971.
Nash, T. The chemical constitution of compounds which protect erythrocytes against freezing damage. J. Gen. Physiol. 46: 167–175; 1962.
Klebe, R. J.; Mancuso, M. G.. Chemicals which promote cell hybridization. Somatic Cell Genet. 7: 473–488; 1981.
Kao, F. T.; Puck, T. T. Genetics of somatic mammalian cells. IV. Properties of Chinese hamster cell mutants with respect to the requirement for proline. Genetics 55: 513–524; 1967.
Akhtar, T.; Pegg, D. E.; Foreman, J. The effect of cooling and warming rates on the survival of cryopreservedl-cells. Cryobiology 16: 424–429; 1979.
Priesler, H. D.; Lyman, G. Differentiation of erythroleukemia cells in vitro: Properties of chemical inducers. Cell Differ. 4: 179–185; 1975.
Bertolini, L.; Tsuei, D.; Holland, J. G.; Friend, C. Variations in the response of cloned murine Friend erythroleukemia cells to different inducers. In Vitro 17: 284–289; 1981.
Friend, C.; Scher, W.; Holland, J. G.; Sato, T. Hemoglobin synthesis in murine virus-induced leukemic cells in vitro: Stimulation of erythroid differentiation by dimethyl sulfoxide. Proc. Natl. Acad. Sci. USA 68: 378–382; 1971.
Reuben, R. A.; Rifkind, R. A.; Marks, P. A. Chemically induced murine erythroleukemic differentiation. Biochim. Biophys. Acta 605: 325–346; 1980.
Norwood, T. H.; Zeigler, C. J.; Martin, G. M. Dimethyl sulfoxide enhances polyethylene glycolmediated somatic cell fusion. Somatic Cell Genet. 2: 263–270; 1976.
Priesler, H. D.; Christoff, G.; Taylor, E. Cryoprotective agents as inducers of erythroleukemic cell differentiation in vitro. Blood 47: 363–368; 1976.
Klebe, R. J.; Mancuso, M. G. Enhancement of poly(ethylene glycol) mediated cell hybridization by inducers of erythroleukemia cell differentiation. Somatic Cell Genet. 8: 723–730; 1982.
Tamaka, M.; Levy, J.; Terada, M.; Breslow, R.; Rifkind, R. A.; Marks, P. A. Induction of erythroid differentiation in murine virus infected erythroleukemia cells by high polar compounds. Proc. Natl. Acad. Sci. USA. 72: 1003–1006; 1975.
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This study was supported, in part, by grants CA 33074 from the National Cancer Institute, Bethesda, MD, and GM 31056 from the National Institutes of Health, Bethesda, MD.
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Klebe, R.J., Mancuso, M.G. Identification of new cryoprotective agents for cultured mammalian cells. In Vitro 19, 167–170 (1983). https://doi.org/10.1007/BF02618055
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DOI: https://doi.org/10.1007/BF02618055