Skip to main content
SpringerLink
Log in

Dimethyl sulfoxide enhances polyethylene glycol-mediated somatic cell fusion

  • Published:
Somatic Cell Genetics

Abstract

The efficiency of fusion of human diploid cells by polyethylene glycol was greatly enhanced by addition of dimethyl sulfoxide. The extent of fusion was directly proportional to the concentrations of both of these compounds. At all except the highest concentrations, cell loss was moderate to minimal and perturbation of cell cycle function as measured by [3H]thymidine labeling indices and mitotic indices was minimal in the surviving cells. This technique is potentially useful for heterokaryon studies as well as for the isolation of hybrids of mammalian somatic cells.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literature cited

  1. Kao, K. N., and Michaylick, M. R. (1974).Planta 115:355–367.

    Google Scholar 

  2. Kao, K. N., Constabel, F., Michaylick, M. R., and Ganberg, O. L. (1974).Planta 120:215–217.

    Google Scholar 

  3. Constabel, F., and Kao, K. N. (1974).Can. J. Bot. 52:1603–1606.

    Google Scholar 

  4. Pontecorvo, G. (1975).Somat. Cell Genet. 1:397–400.

    PubMed  Google Scholar 

  5. Davidson, R. L., and Gerald, P. S. (1976).Somat. Cell Genet. 2:165–176.

    PubMed  Google Scholar 

  6. Harris, H., and Watkins, J. F. (1965).Nature 205:640–646.

    PubMed  Google Scholar 

  7. Croce, C., Sarvieki, W., Kritchevsky, D., and Koprowski, H. (1971).Exp. Cell Res. 67:427–435.

    PubMed  Google Scholar 

  8. Kenny, G. C. (1975). InMicrobiology, (ed.) Schlessinger, (American Society of Microbiology, Washington D.C.) pp. 32–36.

    Google Scholar 

  9. Kenny, G. E. (1969).J. Bacterial 98:1044–1055.

    Google Scholar 

  10. Rao, P. N., and Johnson, R. T. (1970).Nature 225:159–164.

    PubMed  Google Scholar 

  11. Hoehn, H., Bryant, E. M., Johnston, P., Norwood, T. H., and Martin, G. M. (1975).Nature 258:608–610.

    PubMed  Google Scholar 

  12. Stanley, S. W. (1971). InDimethyl Sulfoxide, (ed.) Jacob, S. W., Rosenbaum, E. E., and Wood, D. C. (Marcel Dekker, New York) pp. 99–112.

    Google Scholar 

  13. Ashwood-Smith, M. J. (1971). InDimethyl Sulfoxide, (ed.) Jacob, S. W., Rosenbaum, E. E., and Wood, D. C. (Marcel Dekker, New York) pp. 147–187,

    Google Scholar 

  14. Nash, T. (1966). InCryobiology, (ed.) Merryman, H. T. (Academic Press, New York) pp. 279–311,

    Google Scholar 

  15. Rammler, D. H. (1971). InDimethyl Sulfoxide, (ed.) Jacob, S. W., Rosenbaum, E. E., and Wood, D. C. (Marcel Dekker, New York) pp. 189–206.

    Google Scholar 

  16. Kirk, R. G., and Tosteson, D. C. (1973).J. Membr. Biol. 12:273–285.

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Pathology, University of Washington, 98195, Seattle, Washington

    T. H. Norwood, C. J. Zeigler & G. M. Martin

Authors
  1. T. H. Norwood
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. C. J. Zeigler
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. G. M. Martin
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Norwood, T.H., Zeigler, C.J. & Martin, G.M. Dimethyl sulfoxide enhances polyethylene glycol-mediated somatic cell fusion. Somat Cell Mol Genet 2, 263–270 (1976). https://doi.org/10.1007/BF01538964

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01538964

Keywords

Search

Navigation