Summary
Germ-pore-containing ovule sections, excised from data palm (Phoenix dactylifera) fruit 2 to 3 months after pollination, produced callus in a medium enriched with 2,4-D. Reculture of callus in auxin-less medium resulted in development of numerous asexual embryos. Callus development and asexual embryogenesis also were observed inHoweia forsteriana andChamaedorea costaricana embryo explants, although not as profusely as inP. dactylifera.
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The research wa supported in part by NSF Grant OIP 75-10390, the Elvenia J. Slosson Fellowship in Ornamental Horticulture, and grants-in-aid from Keeline-Wilcox Company, Irvine, California, and Dr. Donald Shields of Brea, California. Plant materials also were provided by the Keeline-Wilcox Company, Dr. Donald Shields, and the U.S. Data and Citrus Station, Indio, California.
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Reynolds, J.F., Murashige, T. Asexual embryogenesis in callus cultures of palms. In Vitro 15, 383–387 (1979). https://doi.org/10.1007/BF02616146
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DOI: https://doi.org/10.1007/BF02616146