Skip to main content
SpringerLink
Log in

In vitro culture of microsporophylls and megagametophyte tissue of pinus

  • Published:
In Vitro Aims and scope Submit manuscript

Summary

Microsporophylls ofPinus resinosa and megagametophyte tissue ofPinus nigra var.austriaca andPinus mugo var.mughus were cultured on Brown and Lawrence's medium. The microsporophylls formed a mixed haploid-diploid callus, and the megagametophyte tissue a purely haploid callus. Subcultures were established of the calluses. The mode of development of haploid callus and various types of organization in the calluses are described. Chromosome stickiness occurred in old calluses.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Bonga, J. M., and D. P. Fowler. 1970. Growth and differentiation in gametophytes ofPinus resinosa cultured in vitro. Can. J. Bot. 48: 2205–2207.

    Article  Google Scholar 

  2. Sharp, W. R., R. S. Raskin, and H. E. Sommer. 1972. The use of nurse culture in the development of haploid clones in tomato. Planta 104: 357–361.

    Article  Google Scholar 

  3. Brown, C. L., and R. H. Lawrence. 1968. Culture of pine callus on a defined medium. Forest Sci. 14: 62–64.

    Google Scholar 

  4. Simak, M., and C. Happel. 1966. Vorbehandlung der Koniferen Samen für Chromosomenuntersuchungen. Silvae Genet. 15: 38–41.

    Google Scholar 

  5. Gerlach, D. 1969.Botanische Mikrotechnik. Georg Thieme Verlag. Stuttgart.

    Google Scholar 

  6. Chamberlain, C. J. 1935.Gymnosperms, Structure and Evolution. 1966 ed. Dover Publications, Inc., New York.

    Google Scholar 

  7. Konar, R. N., and S. Ramchandani. 1958. The morphology and embryology ofPinus wallichiana Jack. Phytomorphology 8: 328–346.

    Google Scholar 

  8. Bennici, A., M. Buiatti, and F. D'Amato. 1968. Nuclear conditions in haploidPelargonium in vivo and in vitro. Chromosoma 24: 194–201.

    Article  Google Scholar 

  9. Niizeki, H., and K. Oono. 1971. Rice plannts obtained by anther culture. In:Les Cultures de Tissus de Plantes. Edition du CNRS. Paris, pp. 251–257.

    Google Scholar 

  10. Tulecke, W. 1957. The pollen ofGinkgo biloba: in vitro culture and tissue formation. Am. J. Bot. 44: 602–608.

    Article  Google Scholar 

  11. Tulecke, W., and N. Sehgal. 1963. Cell proliferation from the pollen ofTorreya nucifera. Contrib. Boyce Thompson Inst. Plant Res 22: 153–163.

    Google Scholar 

  12. LaRue, C. D. 1954. Studies on growth and regeneration in gametophytes and sporophytes of gymnosperms. Brookhaven Symp. Biol. 6: 187–208.

    Google Scholar 

  13. Engvild, K. C., I. Linde-Laursem, and A. Lundquist. 1972. Anther cultures ofDatura innoxia: flower bud stage and embryoid level of ploidy. Hereditas 72: 331–332.

    Article  Google Scholar 

  14. Illies, Z. M. 1964. Auftreten haploider Keimlinge beiPicea abies. Naturwissenschaften 51: 442.

    Article  Google Scholar 

  15. Polheim, F. 1968.Thuja gigantea gracilis Beissn.—ein Haplont unter den Gymnospermen. Biol. Rundschau 6: 84–86.

    Google Scholar 

  16. Konar, R. N. 1963. Studies on submerged callus culture ofPinus gerardiana Wall. Phytomorphology 13: 165–169.

    Google Scholar 

  17. Durzan, D. J., and F. C. Steward. 1970. Morphogenesis in cell cultures of gymnosperms: some growth patterns. Invited paper, Internat. Union of Forest Res. Organ., Sect. 22., Helsinki. 1970.

  18. Borchert, R. 1968. Spontane diploidisierung in Gewebekulturen des Megagametophyten vonPinus lambertiana. Z. Pflanzenphysiol. 59: 389–392.

    Google Scholar 

  19. Tulecke, W. 1967. Studies on tissue cultures derived fromGinkgo biloba L. Phytomorphology 17: 381–386.

    Google Scholar 

  20. Norstog, K., and E. Rhamstine. 1967. Isolation and culture of haploid and diploid cycad tissues. Phytomorphology 17: 374–381.

    Google Scholar 

  21. Gautheret, R. 1956. Sur les Phénomènes d'histogenèse dans les cultures de tissus dePinus strobus L. C. R. Acad. Sci. [D] Paris 242: 3108–3110.

    Google Scholar 

  22. Andersson, E. 1947. A case of asyndesis inPicea abies. Hereditas 33: 301–347.

    Article  Google Scholar 

  23. Eriksson, G. 1968. Temperature respons of pollen mother cells in Larix and its importance for pollen formation. Stud. Forest. Suecica 63: 5–131.

    Google Scholar 

  24. Runquist, E. W. 1968. Meiotic investigations inPinus silvestris (L.). Hereditas 60: 77–128.

    Article  Google Scholar 

  25. Dogra, P. D. 1967. Seed sterility and disturbances in embryogeny in conifers with particular reference to seed testing and tree breeding inPinaceae. Stud. Forest. Suecica 45: 5–97.

    Google Scholar 

  26. Simak, M., A. Gustafsson, and K. Ching. 1968. Occurrence of a mosaic-aneuploid in polyembryonic Norway spruce seed. Stud. Forest. Suecica 67: 3–8.

    Google Scholar 

  27. Nägl, W. 1965. Karyologische Anatomie der Samenanlage vonPinus silvestris. Österreichische Bot. Z. 112: 359–370.

    Article  Google Scholar 

  28. Storey, W. B. 1968. Somatic reduction inCycads. Science 159: 648–650.

    Article  PubMed  CAS  Google Scholar 

  29. Torrey, J. G. 1959. Experimental modification of development in the root. In: D., Rudnick (Ed.),Cell, Organism, and Milieu. Ronald Press, New York, pp. 189–222.

    Google Scholar 

  30. Jackson, W. T. 1972. Regulation of mitosis III. Cytological effects of 2, 4, 5-trichlorophenoxyacetic acid and of dioxin contaminants in 2,4,5-T formulations. J. Cell Sci. 10: 15–25.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Canadian Forestry Service, P.O. Box 4000, Fredericton, New Brunswick, Canada

    J. M. Bonga

Authors
  1. J. M. Bonga
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bonga, J.M. In vitro culture of microsporophylls and megagametophyte tissue of pinus. In Vitro 9, 270–277 (1974). https://doi.org/10.1007/BF02616073

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02616073

Key words

Search

Navigation