Summary
A medium for optimal growth of embryos of Jamaican Tall and Green Malayan Dwarf varieties of coconut palm was developed. The liquid basal Murashige and Skoog medium was supplemented with coconut milk, IAA and 2IP. Activated charcoal improved embryo growth on agar medium. A single callus line was initiated from solid endosperm and subcultured on basal Schenk and Hildebrandt medium supplemented with 2 mg per 1 NAA. Attempts at inducing organogenesis in the callus were unsuccessful. No vascular tissue was present. The callus was aneuploid with the chromosome number=8 (normal 2n=32).
Similar content being viewed by others
References
Rommey, D. H. 1976. Second meeting of the International Council on Lethal Yellowing. Principles 20: 57–69.
Fisher, J. B. 1975. Environmental impact of lethal yellowing disease of coconut plams. Environ. Conserv. 2: 299–302.
Tsai, J. H. 1975. Transmission studies of three suspected insect vectors of lethal yellowing of coconut palms. FAO Plant Prot. Bull. 23: 140–145.
Tsai, J. H. Lethal yellowing of coconut palm: Search for a vector. In: K. F. Harris and K. Maramorosch (Eds.),Vectors of Plant Disease Agents. Vol. 3. Academic Press, New York, in press
Abraham, A., and K. J. Thomas. 1962. A note on thein vitro culture of excised coconut embryos. Indian Coconut J. 15: 84–88.
Cutter, V. M., and K. S. Wilson. 1954. Effect of coconut endosperm and other growth stimulants upon the development in vitro of embryos ofCocos nucifera. Bot. Gaz. 115: 234–240.
De Guzman, E. V., A. G. del Rosario, and E. C. Eusebio. 1971. The growth and development of coconut “Makapuno” embryoin vitro. III. Resumption of root growth in high sugar media. Philipp. Agric. 53: 566–579 (and preceding papers in this series).
Rabéchault, H., J. Ahée, and G. Guénin. 1970. Colonies cellulaires et formes embryoïds obtenuesin vitro à partir cultures d'embryons de Palmier à huile (Elaeis guineensis Jacq. var. dura Becc.) C. R. Acad. Sci. 270D: 3067–3070.
Smith, W. K., and J. A. Thomas. 1973. The isolation andin vitro cultivation of cells ofElaeis guineensis. Oléagineux 28: 123–127.
Apavatjrut, P., and J. Blake. 1977. Tissue culture of stem explants of coconut (Cocos nucifera L.). Oléagineux 32: 267–271.
Murashige, T., and F. Skoog. 1962. A revised medium for rapid growth and bioassay with tobacco tisue culture. Physiol. Plant 15: 485–497.
Schenk, R. U., and A. C. Hildebrandt. 1972. Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Can. J. Bot. 50: 199–204.
White, P. R. 1963.The Cultivation of Animal and Plant Cells. 2nd Ed. Ronald Press, New York.
Jensen, W. A. 1962.Botanical Histochemistry. Freeman, San Francisco.
Wang, P. J., and L. C. Huang. 1976. Beneficial effects of activated charcoal in plant tissue and organ cultures. In Vitro 12: 260–262.
Moore, H. E., Jr. 1973. The major groups of plams and their distribution. Gentes Herbarum 11: 27–141.
Cutter, V. M., K. S. Wilson, and B. Freeman. 1955. Nuclear behavior and cell formation in the developing endosperm ofCocos nucifera. Am. J. Bot. 42: 109–115.
Dutt, M. 1953. Dividing nuclei in coconut milk. Nature 171: 799–800.
Author information
Authors and Affiliations
Additional information
Florida Agricultural Experiment Stations Journal Series No. 542.
The research was supported in part by the Horticultural Research Institute (to J. H. T.) and the American Philosophical Society (to J.B.F.).
Rights and permissions
About this article
Cite this article
Fisher, J.B., Tsai, J.H. In vitro growth of embryos and callus of coconut palm. In Vitro 14, 307–311 (1978). https://doi.org/10.1007/BF02616041
Issue Date:
DOI: https://doi.org/10.1007/BF02616041