Summary
A continuous attached cell strain has been developed from the IPRI-CF-124 line of the spruce budworm,Choristoneura fumiferana. This was done by discarding suspended cells at each passage, rinsing attached cells with 0.05% trypsin and using only the strongly attached cells for subculturing. The method is very effective in that the proportion of attached cells increased from 6% in the parent cell line to 97% in the new cell strain after 20 passages. The attachment and growth properties are stable after storage of cells in liquid nitrogen. The new cell strain is designated IPRI-CF-124T and has a population doubling time comparable to that of the parent cell line.
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References
Vaughn, J. L. 1976. The production of nuclear polyhedrosis viruses in large-volume cell cultures. J. Invertebr. Pathol. 28: 233–237.
Sohi, S. S. 1973. Establishment of cultures ofMalacosoma disstria Hübner (Lepidoptera: Lasiocampidae) hemocytes in a hemolymph-free medium. Proc. 3rd Int. Colloq. Invertebr. Tissue Culture, Smolenice, Czechoslovakia (1971): 27–39.
Goodwin, R. H. 1975. Insect cell culture: improved media and methods for initiating attached cell lines from the Lepidoptera. In Vitro 11: 369–378.
Hink, W. F. 1970. Established insect cell line from the cabbage looper,Trichoplusia ni. Nature 226: 466–467.
Grace, T. D. C. 1962. Establishment of four strains of cells from insect tissues grownin vitro. Nature 195: 788–789.
Sohi, S. S. 1973.In vitro cultivation of larval tissues ofChoristoneura fumiferana (Clemens) (Lepidoptera: Tortricidae). Proc. 3rd Int. Colloq. Invertebr. Tissue Culture, Smolenice, Czechoslovakia (1971): 75–92.
Arif, B. M., S. S. Sohi, and J. Krywienczyk. 1976. Replication of alkali-released NPV in a cell line ofChoristoneura fumiferana. Proc. 1st Int. Colloq. Invertebr. Pathol. and 9th Annual Meeting Soc. Invertebr. Pathol., Kingston, Ontario (1976): 108–112.
Rinaldini, L. M. 1959. An improved method for the isolation and quantitative cultivation of embryonic cells. Exp. Cell. Res. 16: 477–505.
Weiss, L. 1960. The adhesion of cells. Int. Rev. Cytol. 9: 187–225.
Weiss, L., and D. L. Kapes 1966. Observations on cell adhesion and separation following enzyme treatment. Exp. Cell. Res. 41: 601–608.
Cassiman, J. J., and M. R. Bernfield. 1975. Transformation-induced alterations in fibroblast adhesion: masking by trypsin treatment. Exp. Cell. Res. 91: 31–35.
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Bilimoria, S.L., Sohi, S.S. Development of an attached strain from a continuous insect cell line. In Vitro 13, 461–466 (1977). https://doi.org/10.1007/BF02615136
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DOI: https://doi.org/10.1007/BF02615136