Summary
The reagentp-nitrobenzyloxycarbonyl chloride (PNZ-CI) was used for pre-column derivatization of biogenic amines (BAs) at ambient temperature followed by reversed-phase, liquid-chromatographic separation of the derivatives. Optimized derivatization of samples was achieved within 10 min in borate buffer by adding PNZ-Cl in acetonitrile (MeCN). Excess reagent was scavenged by subsequent addition of glycine in water. For LC a Superspher® RP-18e column and gradient elution using a ternary gradient system containing sodium acetabe buffer (pH 6.1), sodium acetate buffer (pH 4.3) and MeCN, were used. The PNZ-derivatives were quantified by their UV-absorption at 265 nm. Detection limits of BAs were approximately 62–1000 μg L−1 (injected amounts: 53–850 pg) at a signal-to-noise ratio of 3:1. The coefficients of determination were 0.9906–0.9992. Diaminohexane was used as internal standard. Recoveries of BAs ranged from 78–93% depending on the food matrix. This method was applied to the quantitative determination of 2-phenylethylamine, tryptamine, serotonin, putrescine, histamine, cadaverine, tyramine, spermidine, and spermine, in beer, wine, vinegars, and lactic fermented cabbage juice.
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Abbreviations
- Cad:
-
cadaverine (1,5-diaminopentane)
- Dhx:
-
1,6-diaminohexane (internal standard)
- Him:
-
histamine
- Ocp:
-
octopamine
- Pea:
-
2-phenylethylamine
- Put:
-
putrescine (1,4-diaminobutane)
- Seo:
-
serotonin
- Spd:
-
spermidine
- Spm:
-
spermine
- Trm:
-
tryptamine
- Tym:
-
tyramine
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Kirschbaum, J., Meier, A. & Brückner, H. Determination of biogenic amines in fermented beverages and vinegars by pre-column derivatization withpara-nitrobenzyloxycarbonyl chloride (PNZ-Cl) and reversed-phase LC. Chromatographia 49, 117–124 (1999). https://doi.org/10.1007/BF02575272
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DOI: https://doi.org/10.1007/BF02575272