Summary
Seeds ofVicia faba L. var.minor (2n=12) were submerged in tap water for 12 to 96 hours. After submersion and recovery times of 48 to 144 hours root tips were fixed in intervals of 24 hours. The presoaking of seeds before germination led to an increase of the spontaneous chromosomal aberration rate. Up to 65% of all cells in the root tips showed fragments, chromatid-translocations and triradials after 72 hours of presoaking and up to 12% of all chromosomes showed breaks or participated in chromatidal reunions. Submersion of seeds for 12 hours already caused a statistically significant increase of chromosomal aberrations in comparison to the controls. The maximum of aberrations was found after a recovery period of 120 hours, afterwards the aberration rate dropped.
Parallel experiments with the N2-atmosphere showed that the increase in aberration rate is caused by metabolic changes produced by anaerobiosis. Probably the changed metabolic conditions led to the formation of automutagenic substances. The distribution of breaks between the mand SAT-chromosomes was not at random (theoretical expectation 5∶2); more breaks appeared in the mthan in the SAT chromosomes (actual relation 5∶1,1). The breaks in the mand SAT chromosomes were localized in a few special regions.
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Rieger, R., Michaelis, A. Cytologische und stoffwechselphysiologische Untersuchungen am aktiven Meristem der Wurzelspitze von Vicia faba L.. Chromosoma 9, 238–257 (1957). https://doi.org/10.1007/BF02568078
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DOI: https://doi.org/10.1007/BF02568078