Abstract
Chondrocytes from 13-day-old embryonic chick sterna were cultured for 6 days in the presence of vitamin A (10 I.U./ml). Vitamin A treated chondrocytes became flattened and stellate within the first 24 hours of culture. After 6 days of culture, the treated cells contained 59% less DNA than the untreated controls. Sulfated glycosaminoglycan synthesis was inhibited 84%, and a greater percentage of GAG was secreted into the medium (90%) than in control cultures (78%). Vitamin A inhibited cell proliferation, and to varying degrees, RNA and protein synthesis, and these effects are dose dependent.
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Vasan, N.S., Lash, J.W. Chondrocyte metabolism as affected by vitamin A. Calc. Tis Res. 19, 99–107 (1975). https://doi.org/10.1007/BF02563995
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DOI: https://doi.org/10.1007/BF02563995