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Dexamethasone and 1,25(OH)2 vitamin D3 modulate the synthesis of insulin-like growth factor-I in osteoblast-like cells

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Summary

In the present study, we have shown that insulin-like growth factor-I (IGF-I) was released by primary cultures of rat osteoblast-like (ROB) cells into the conditioned medium (CM). Dexamethasone (DEX) caused a dose-dependent inhibition of the IGF-I. At 10−8 M, DEX reduced IGF-I level to 70% of the control value (P<0.05); at 10−7 M DEX, the IGF-I level was further reduced to 60% of the control (P<0.01). The active vitamin D metabolite 1,25-dihydroxycholecalceferol [1,25(OH)2D2] slightly increased the IGF-I level, but the increase was not statistically significant. However, in combined treatments of 10−7 M DEX and 10−8 M of 1,25(OH)2D3, the inhibition of DEX was partially antagonized by the presence of 1,25(OH)2D3. Studies with metabolically radiolabeled IGF-I by immunoprecipitation indicated the changes of IGF-I in the CM reflected synthesis of the protein by the cells. The alteration of IGF-I level may mediate some of the actions of these steroid hormones on bone cells.

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Authors and Affiliations

  1. California Biotechnology Inc., 94043, Mountain View, CA, USA

    Theresa L. Chen & Joanne Bednarz Mallory

  2. Department of Pediatrics, Stanford University, 94305, Stanford, CA, USA

    Raymond L. Hintz

Authors
  1. Theresa L. Chen
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  2. Joanne Bednarz Mallory
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  3. Raymond L. Hintz
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Chen, T.L., Mallory, J.B. & Hintz, R.L. Dexamethasone and 1,25(OH)2 vitamin D3 modulate the synthesis of insulin-like growth factor-I in osteoblast-like cells. Calcif Tissue Int 48, 278–282 (1991). https://doi.org/10.1007/BF02556380

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  • DOI: https://doi.org/10.1007/BF02556380

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