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Acclimation of aquatic microbial communities to Hg(II) and CH3Hg+ in polluted freshwater ponds

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Abstract

The relationship of mercury resistance to the concentration and chemical speciation of mercurial compounds was evaluated for microbial communities of mercury-polluted and control waters. Methodologies based on the direct viable counting (DVC) method were adapted to enumerate mercury-resistant communities. Elevated tolerance to Hg(II) was observed for the microbial community of one mercury-polluted pond as compared to the community of control waters. These results suggest an in situ acclimation to Hg(II). The results of the methylmercury resistance-DVC assay suggested that minimal acclimation to CH3Hg+ occurred since similar concentrations of CH3HgCl inhibited growth of 50% of organisms in both the control and polluted communities. Analyses of different mercury species in pond waters suggested that total mercury, but not CH3Hg+ concentrations, approached toxic levels in the polluted ponds. Thus, microbial acclimation was specific to the chemical species of mercury present in the water at concentrations high enough to cause toxic effects to nonacclimated bacterial communities.

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References

  1. American Public Health Association (1989) Standard methods for the examination of water and wastewater. 17th ed. Washington DC

  2. Barkay T (1987) Adaptation of aquatic microbial communities to Hg2+ stress. Appl Environ Microbiol 53:2725–2732

    CAS  PubMed  Google Scholar 

  3. Barkay T, Liebert C, Gillman G (1989) Hybridization of DNA probes with whole-community genome for detection of genes that encode microbial responses to pollutants:mer genes and Hg2+ resistance. Appl Environ Microbiol 55:1574–1577

    CAS  PubMed  Google Scholar 

  4. Barkey T, Tripp SC, Olson BH (1985) Effect of metal-rich sewage sludge appliction on the bacterial communities of grasslands. Appl Environ Microbiol 49:333–337

    Google Scholar 

  5. Barkay T, Turner R, VandenBrook A, Liebert C (1991) The relationships of Hg(II) volatilization from a freshwater pond to the abundance ofmer genes in the gene pool of the indigenous microbial community. Microb Ecol 21:151–161

    CAS  Google Scholar 

  6. Benes P, Gjessing ET, Steinnes E (1976) Interactions between humus and trace elements in fresh water. Water Res 10:711–716

    Article  CAS  Google Scholar 

  7. Bloom NS (1989) Determination of picogram levels of methylmercury by aqueous phase ethylation, followed by cryogenic gas chromatography with cold vapour atomic fluorescence detection, Can J Fish Aquat Sci 46:1131–1140

    CAS  Google Scholar 

  8. Boethling RS, Alexander M (1979) Effect of concentration of organic chemicals on their biodegradation by natural microbial communities. Appl Environ Microbiol 37:1211–1216

    CAS  PubMed  Google Scholar 

  9. Chen S, Alexander M (1989) Reasons for acclimation for 2,4-D biodegradation in lake water. J Environ Qual 18:153–156

    CAS  Google Scholar 

  10. Dean-Ross D, Mills AL (1989) Bacterial community structure and function along a heavy metal gradient. Appl Environ Microbiol 55:2002–2009

    CAS  PubMed  Google Scholar 

  11. Dougherty TJ, Saukkonen JJ (1985) Membrane permeability changes associated with DNA gyrase inhibitors inEscherichia coli. Antimicrob Agents Chemother 28:200–206

    CAS  PubMed  Google Scholar 

  12. Drlica K (1984) Biology of bacterial deoxyribonucleic acid topoisomerases. Microbiol Rev 48:273–289

    CAS  PubMed  Google Scholar 

  13. Duxbury T (1981) Toxicity of heavy metals to soil bacteria. FEMS Microbiol Lett 11: 217–220

    Article  CAS  Google Scholar 

  14. Duxbury T (1986) Ecological aspects of heavy metal responses in microorganisms. Adv Microb Ecol 8:185–235

    Google Scholar 

  15. Duxbury T, Bicknell B (1983) Metal tolerant bacterial populations from natural and metal-polluted soils. Soil Biol Biochem 15:243–250

    Article  CAS  Google Scholar 

  16. Duxbury T, McIntyre R (1989) Population density-dependent metal tolerance: One possible basis and its ecological implications. Microb Ecol 18:187–197

    Article  CAS  Google Scholar 

  17. Fitzgerald WF, Gill GA (1979) Subnanogram determination of mercury by two-stage gold amalgamation and gas-phase detection applied to atmospheric analysis. Anal Chem 51:1714–1720

    Article  CAS  Google Scholar 

  18. Furutani A, Rudd JWM (1980) Measurement of mercury methylation in lake water and sediment samples. Appl Environ Microbiol 40:770–776

    CAS  PubMed  Google Scholar 

  19. Ghosal D, You IS, Chatterjee DK, Chakrabarty AM (1985) Microbial degradation of halogenated compounds. Science 228:135–142

    Article  CAS  PubMed  Google Scholar 

  20. Goss WA, Deitz WH, Cook TM (1964) Mechanism of action of nalidixic acid onEscherichia coli, J Bacteriol 88:1112–1118

    CAS  PubMed  Google Scholar 

  21. Hobbie JE, Daley RJ, Hasper S (1977) Use of Nucleopore filters for counting bacteria by fluorescence microscopy. Appl Environ Microbiol 33:1225–1228

    CAS  PubMed  Google Scholar 

  22. Jonas RB, Gilmour CC, Weir DL, Tuttle JH (1984) Comparison of methods to measure acute metal and organometal toxicity to natural aquatic microbial communities. Appl Environ Microbiol 47:1005–1011

    CAS  PubMed  Google Scholar 

  23. Kiene RP, Capone DG (1986) Stimulation of methanogenesis by Aldicarb and several other n-methyl carbamate pesticides. Appl Environ Microbiol 51:1247–1251

    CAS  PubMed  Google Scholar 

  24. Kogure K, Simidu U, Taga N (1979) A tentative direct microscopic method for counting living marine bacteria. Can J Microbiol 25:415–420

    CAS  PubMed  Google Scholar 

  25. Lewis DL, Kolling HP, Hodson RE (1986) Nutrient limitation and adaptation of microbial populations to chemical transformations. Appl Environ Microbiol 51:498–603

    Google Scholar 

  26. Liebert C, Barkay T (1988) A direct viable counting method for measuring tolerance of aquatic microbial communities to Hg2+. Can J Microbiol 34:1090–1095

    Article  CAS  Google Scholar 

  27. Nakamura K, Sakamoto M, Uchiyama Y, Yagi O (1990) Organomercurial-volatilizing bacteria in the mercury-polluted sediment of Minamata Bay, Japan. Appl Environ Microbiol 56: 304–305

    CAS  PubMed  Google Scholar 

  28. Nelson JD, Colwell RR (1975) The ecology of mercury-resistant bacteria in Chesapeake Bay. Microb Ecol 1:191–218

    Article  CAS  Google Scholar 

  29. Olson BH, Barkay T (1986) Feasibility of using bacterial resistance to metals for mineral exploration. In: Carlisle WB, Kaplan I, Watterson J (eds) Mineral exploration, biological systems and organic matter. Vol. 5. Prentice-Hall, Inc., Englewood Cliffs NJ, pp 311–327

    Google Scholar 

  30. Olson BH, Thornton I (1982) The resistance patterns to metals of bacterial populations in contaminated land. J Soil Sci 33:271–277

    Article  CAS  Google Scholar 

  31. Ramlal PS, Rudd JWM, Furutani A, Xun L (1985) The effect of pH on methylmercury production and decomposition in lake sediments. Can J Fish Aquat Sci 42:685–692

    Article  CAS  Google Scholar 

  32. Robinson JB, Tuovinen OH (1984) Mechanisms of microbial resistance and detoxification of mercury and organomercury compounds: Physiological, biochemical and genetic analyses. Microbiol Rev 48:95–124

    CAS  PubMed  Google Scholar 

  33. Schmidt E, Hellwig M, Knackmuss HJ (1983) Degradation of chlorophenols by a defined mixed microbial community. Appl Environ Microbiol 46:1038–1044

    CAS  PubMed  Google Scholar 

  34. Schmidt SK, Alexander M (1985) Effects of dissolved organic carbon and second substrates on the biodegradation of organic compounds at low concentrations. Appl Environ Microbiol 49:822–827

    CAS  PubMed  Google Scholar 

  35. Singh A, Yu FP, McFeters GA (1990) Rapid detection of chlorine-induced bacterial injury by the direct viable count method using image analysis. Appl Environ Microbiol 56:389–394

    CAS  PubMed  Google Scholar 

  36. Smolenski WJ, Suflita JM (1987) Biodegradation of cresol isomers in anoxic aquifers. Appl Environ Microbiol 53:710–716

    CAS  PubMed  Google Scholar 

  37. Spain JC, Pritchard PH, Bourquin AW (1980) Effects of adaptation of biodegradation rates in sediment/water cores from estuarine and freshwater environments. Appl Environ Microbiol 40:726–734

    CAS  PubMed  Google Scholar 

  38. spain JC, Van Veld PA (1983) Adaptation of natural microbial communities to degradation of xenobiotic compounds: Effects of concentration, exposure time, inoculum, and chemical structure. Appl Environ Microbiol 45:428–435

    CAS  PubMed  Google Scholar 

  39. Steffan RJ, Korthals ET, Winfrey MR (1988) Effects of acidification on mercury methylation, demethylation, and volatilization in sediments from an acid-susceptible lake. Appl Environ Microbiol 54:2003–2009

    CAS  PubMed  Google Scholar 

  40. Summers AO (1986) Organization, expression, and evolution of genes for mercury resistance. Ann Rev Microbiol 40:607–634

    Article  CAS  Google Scholar 

  41. Tabor S, Neihof RA (1984) Direct determination of activities for microorganisms of Chesapeake Bay populations. Appl Environ Microbiol 48:1012–1019

    PubMed  CAS  Google Scholar 

  42. Timoney JF, Port J, Giles J, Spanier J (1978) Heavy-metal and antibiotic resistance in the bacterial flora of sediments of New York Bight. Appl Environ Microbiol 36:465–472

    CAS  PubMed  Google Scholar 

  43. Turner RR, Olsen CR, Wilcox WJ (1985) Environmental fate of Hg and137Cs discharges from Oak Ridge facilities. In: Proc. 18th Conf. Trace Substances in Environmental Health, June 4–7, 1984, Columbia, MO

  44. U.S. Environmental Protection Agency (1974) Methods for chemical analyses of water and wastes. U.S. Environmental Protection Agency, Washington DC, pp 118–137

    Google Scholar 

  45. Ventullo RM, Larson RJ (1986) Adaptation of aquatic microbial communities to quaternary ammonium compounds. Appl Environ Microbiol 51:356–361

    CAS  PubMed  Google Scholar 

  46. Wiggins BA, Alexander M (1988) Role of chemical concentration and second carbon sources in acclimation of microbial communities for biodegradation. Appl Environ Microbiol 54: 2803–2807

    CAS  PubMed  Google Scholar 

  47. Wiggins BA, Jones SH, Alexander M (1987) Explanations for the acclimation period preceding the mineralization of organic chemicals in aquatic environments. Appl Environ Microbiol 53: 791–796

    CAS  PubMed  Google Scholar 

  48. Winfrey MR, Rudd JWM (1990) Environmental factors affecting the formation of methylmercury in low pH lakes. Environ Toxicol and Chem 9:853–869

    CAS  Google Scholar 

  49. Zelibor JL, Doughten MW, Grimes DJ, Colwell RR (1987) Testing for bacterial resistance to arsenic in monitoring well water by the direct viable counting method. Appl Environ Microbiol 53:2929–2934

    CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Technical Resources, Inc, 32561, Gulf Breeze, Florida, USA

    C. A. Liebert

  2. Microbial Ecology and Biotechnology Branch, Environmental Research Laboratory, U.S. Environmental Protection Agency, 32561, Gulf Breeze, Plorida

    T. Barkay

  3. Environmental Science Division, Oak Ridge National Laboratory, 37831, Oak Ridge, Tennessee, USA

    R. R. Turner

Authors
  1. C. A. Liebert
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  2. T. Barkay
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  3. R. R. Turner
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Liebert, C.A., Barkay, T. & Turner, R.R. Acclimation of aquatic microbial communities to Hg(II) and CH3Hg+ in polluted freshwater ponds. Microb Ecol 21, 139–149 (1991). https://doi.org/10.1007/BF02539149

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  • DOI: https://doi.org/10.1007/BF02539149

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