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Dietary fish oil and cytochrome P-450 monooxygenase activity in rat liver and kidney

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Lipids

Abstract

Lauric acid hydroxylation and aminopyrineN-demethylation were studied in kidney and liver microsomes from rats treated with fish oil. Different doses of fish oil containing 20% eicosapentaenoic acid and 10% docosahexaenoic acid were provided daily to the rats for seven days. In all the groups studied, the lauric acid metabolism was higher in kidney microsomes and the aminopyrine metabolism in the liver microsomes. Although no effect on the renal cytochrome P-450 concentration was detectable, all four fish oil doses increased the hepatic concentration of cytochrome P-450 by a mean 27%. The higher fish oil doses used increased the renal and hepatic microsomal metabolism of aminopyrine. The lauric acid metabolism was increased by fish oil only in the liver. Fish oil, a known inducer of fatty acid peroxisomal β-oxidation, also induced microsomal activity. These results show that liver and kidney respond in different ways to dietary factors such as fish oil. In addition, our study would suggest that fish oil increased the activity of two different families of liver cytochrome P-450. The activity of kidney lauric acid 11- and 12-hydroxylation, however, was not modulated by fish oil.

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Abbreviations

ANOVA:

analysis of variance

EPA:

eicosapentaenoic acid

DHA:

docosahexaenoic acid

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Authors and Affiliations

  1. Departmento de Bioquimica, Facultad de Medicina, Universidad de Chile, Independencia 1027 Casilla, 70086, Santiago 7, Chile

    Eleva Valdes, Patricio Vega, Nicolas Avalos & Myriam Orellana

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  1. Eleva Valdes
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  2. Patricio Vega
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  3. Nicolas Avalos
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  4. Myriam Orellana
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Valdes, E., Vega, P., Avalos, N. et al. Dietary fish oil and cytochrome P-450 monooxygenase activity in rat liver and kidney. Lipids 30, 955–958 (1995). https://doi.org/10.1007/BF02537488

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  • DOI: https://doi.org/10.1007/BF02537488

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