Skip to main content
SpringerLink
Log in

Meningioma phospholipid profiles measured by31P nuclear magnetic resonance spectroscopy

  • Article
  • Published:
Lipids

Abstract

Fourteen cases of intracranial meningioma were characterized after chloroform/methanol extraction by31P nuclear magnetic resonance (NMR) spectroscopy at 202.4 MHz. Each phospholipid class detected in the extracts was identified and quantitated in terms of its molar percentage relative to the total phospholipids measured. The following phospholipids were assayed by31P NMR: phosphatidylglycerol, phosphatidic acid, diphosphatidylglycerol, ethanolamine plasmalogen, phosphatidylethanolamine (PE), lysophosphatidylinositol, phosphatidylserine, sphingomyelin, lysophosphatidylcholine (LPC), phosphatidylinositol (PI), sphingosylphosphorylcholine and phosphatidylcholine. In addition, two unidentified phospholipids were detected with resonances at 0.13 and −0.78 ppm, respectively. Three distinct types of spectra were obtained on the extracts and grouped accordingly for comparison purposes. Type 1 tumors showed unusual31P NMR profiles with low levels of PE and PI and elevated levels of LPC; type 2 tumors were characterized by low levels of the ethanolamine phospholipids and near equivalent levels of PI and LPC. The spectra of type 1 and type 2 tumors were characteristic of degenerative cells that lacked membrane permeability associated with loss of ethanolamine plasmalogen in the presence of significant phospholipid turnover. Meningiomas belonging to the third spectral type showed characteristics similar to those of normal tissues with normal levels of PE and ethanolamine plasmalogen, as well as very low levels of LPC relative to PI. Type 3 tumors lacked the characteristic signs of degeneration noted in type 1 and type 2 tumors. The data corroborate and augmentin vivo spectroscopic findings reported earlier and demonstrate the value of31P NMR spectroscopic phospholipid analysis on lipid extracts for the characterization of meningiomas.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

ATP:

adenosine triphosphate

CPLAS:

choline plasmalogen

CPLIP:

choline phospholipid

DPG:

diphosphatidylglycerol, cardiolipin

EDTA:

(ethylenedinitrilo)tetraacetic acid

EPLAS:

ethanolamine plasmalogen

LPC:

lysophosphatidylcholine

LPI:

lysophosphatidylinositol

NMR:

nuclear magnetic resonance

PA:

phosphatidic acid

PC:

phosphatidylcholine

PE:

phosphatidylethanolamine

PG:

phosphatidylglycerol

PI:

phosphatidylinositol

PS:

phosphatidylserine

SM:

sphingomyelin

SPC:

sphingosylphosphorylcholine

U:

unidentified phospholipid

References

  1. Peeling, J., and Sutherland, G. (1992)Magn. Reson. Med. 24, 123–136.

    PubMed  CAS  Google Scholar 

  2. Gill, S.S., Thomas, D.G., Van Bruggen, N., Gadian, D.G., Peden, C.J., Bell, J.D., Cox, I.J., Menon, D.K., Iles, R.A., Bryant, D., and Coutts, G.A. (1990)J. Comput. Assist. Tomogr. 14, 497–504.

    Article  PubMed  CAS  Google Scholar 

  3. Cadoux-Hudson, T.A., Blackledge, M.J., Rajagopalan, B., Taylor, D.J., and Radda, G.K. (1989)Br. J. Cancer. 60, 430–436.

    PubMed  CAS  Google Scholar 

  4. Henriksen, O., Wieslander, S., Gjerris, F., and Jensen, K.M. (1991)Acta Radiol. 32, 95–99.

    PubMed  CAS  Google Scholar 

  5. Bruhn, H., Frahm, J., Gyngell, M.L., Merboldt, K.D., Hanicke, W., Sauter, R., and Hamburger, C. (1989)Radiology 172, 541–548.

    PubMed  CAS  Google Scholar 

  6. Thomsen, C., Jensen, K.E., Achten, E., and Henriksen, O. (1988)Acta Radiol. 29, 77–82.

    Article  PubMed  CAS  Google Scholar 

  7. Heindel, W., Bunke, J., Glathe, S., Steinbrich, W., and Mollevanger, L. (1988)J. Comput. Assist. Tomogr. 12, 907–916.

    Article  PubMed  CAS  Google Scholar 

  8. Oberhaensli, R.D., Hilton-Jones, D., Bore, P.J., Hands, L.J., Rampling, R.P., and Radda, G.K. (1986)Lancet 2, 8–11.

    Article  PubMed  CAS  Google Scholar 

  9. Hubesch, B., Sappey-Marinier, D., Roth, K., Meyerhoff, D.J., Matson, G.B., and Wiener, M.W. (1990)Radiology 174, 401–409.

    PubMed  CAS  Google Scholar 

  10. Just, M., and Thelen, M. (1988)Radiology 169, 779–785.

    PubMed  CAS  Google Scholar 

  11. Daly, P.F., and Cohen, J.S. (1989)Cancer Res. 49, 770–779.

    PubMed  CAS  Google Scholar 

  12. Heindel, W., Heiss, W.D., Bunke, J., Herholz, K., Glathe, S., and Jeske, J. (1988)Proc. Soc. Magn. Res. Med. 2, 717.

    Google Scholar 

  13. Arnold, D.L., Shoubridge, E.A., Feindel, W., and Villemure, J.G. (1987)Can. J. Neurol. Sci. 14, 570–575.

    PubMed  CAS  Google Scholar 

  14. Heindel, W., Bunke, J., Steinbrich, W., and Friedmann, G. (1987)Proc. Soc. Magn. Res. Med. 1, 153.

    Google Scholar 

  15. Tanaka, C., Naruse, S., Horikawa, Y., and Higuchi, T. (1988)Proc. Soc. Magn. Res. Med. 1, 318.

    Google Scholar 

  16. Shoubridge, E.A., Arnold, D.L., Emrich, J.F., Villemure, J.G., and Feindel, W. (1988)Proc. Soc. Magn. Res. Med. 2, 616.

    Google Scholar 

  17. Redmond, O., Stack, J. Scully, M., Dervan, P., Carney, D., and Ennis, J.T. (1988)Symposium on Positron Emission Tomography, p. 16.

  18. Pettegrew, J.W., Moossy, J., Withers, G., McKeag, D., and Panchalingam, K. (1988)J. Neuropathol. Exp. Neurol. 47, 235–248.

    PubMed  CAS  Google Scholar 

  19. Pettegrew, J.W., Withers, G., and Panchalingam, K. (1990) inNMR: Principles and Applications to Biomedical Research (Pettegrew, J.W., ed.) pp. 204–254, Springer-Verlag, New York.

    Google Scholar 

  20. Klunk, W.E., McClure, R.J., and Pettegrew, J.W. (1991)J. Neurochem. 56, 1997–2003.

    Article  PubMed  CAS  Google Scholar 

  21. Bradler, J.E., Barrionuevo, G., Panchalingam, K., McKeag, D., and Pettegrew, J.W. (1991)Synapse 9, 7–13.

    Article  PubMed  CAS  Google Scholar 

  22. Meneses, P., Para, P.F., and Glonek, T. (1989)J. Lipid Res. 30, 458–461.

    PubMed  CAS  Google Scholar 

  23. Folch, J., Lees, M., and Sloane-Stanley, G.H. (1957)J. Biol. Chem. 226, 497–509.

    PubMed  CAS  Google Scholar 

  24. Meneses, P., Navarro, J.N., and Glonek, T. (1993)Int. J. Biochem. 25, 903–910.

    Article  PubMed  CAS  Google Scholar 

  25. Meneses, P., and Glonek, T. (1988)J. Lipid Res. 29, 679–689.

    PubMed  CAS  Google Scholar 

  26. Glonek, T. (1994) inPhosphorus-31 NMR Spectral Properties in Compound Characterization and Structural Analysis (Quin, L.D., and Verkade, J.G., eds.) VCH Publishers, New York.

    Google Scholar 

  27. Edzes, H.T., Teerlink, T., van der Knaap, M.S., and Valk, J. (1992)Magn. Reson. Med. 26, 46–59.

    PubMed  CAS  Google Scholar 

  28. Glonek, T., and Van Wazer, J.R. (1974)J. Magn. Reson 13, 390–391.

    CAS  Google Scholar 

  29. Greiner, J.V., Kopp, S.J., Sanders, D.R., and Glonek, T. (1981)Invest. Ophthalmol. Visual Sci. 21, 700–713.

    CAS  Google Scholar 

  30. Barany, M., and Glonek, T. (1982)Meth. Enzymol. 85B, 624–676.

    Article  Google Scholar 

  31. Capuani, G., Aureli, T., Miccheli, A., Di Cocco, M.E., Ramacci, M.T., and Delfini, M. (1992)Lipids 27, 389–391.

    PubMed  CAS  Google Scholar 

  32. Brenton, D.P., Garrod, P.J., Krywawych, S., Reynolds, E.O., Bachelard, H.S., Cox, D.W., and Morris, P.G. (1985)Lancet 1, 115.

    Article  PubMed  CAS  Google Scholar 

  33. Cerdan, S., Harihara Subramanian, V., Hilberman, M., Core, J., Egan, J., Chance, B., and Williamson, J.R. (1986)Magn. Reson. Med. 3, 432–439.

    PubMed  CAS  Google Scholar 

  34. Burt, C.T., Cohen, S.M., and Barany, M. (1979)Annu. Rev. Biophys. Bioeng. 8, 1–25.

    Article  PubMed  CAS  Google Scholar 

  35. Coleman, A., and Gadian, D.G. (1976)Eur. J. Biochem. 61, 387–396.

    Article  Google Scholar 

  36. Glonek, T., Henderson, T.O., Kruski, A.W., and Scanu, A.M. (1974)Biochim. Biophys. Acta 348, 155–161.

    PubMed  CAS  Google Scholar 

  37. Horrocks, L.A., and Sharma, M. (1982) inPhospholipids (Hawthorne, J.N., and Ansell, G.B., eds.) pp. 71–72, Elsevier Biomedical Press, Amsterdam.

    Google Scholar 

  38. Albert, D.H., and Anderson, C.E. (1977)Lipids 12, 188–192.

    Article  PubMed  CAS  Google Scholar 

  39. Ronen, S.M., and Degani, H. (1992)Magn. Reson. Med. 25, 384–389.

    PubMed  CAS  Google Scholar 

  40. Steen, R.G. (1989)Cancer Res. 49, 4075–4085.

    PubMed  CAS  Google Scholar 

  41. Sachedina, S., Greiner, J.V., and Glonek, T. (1991)Invest. Ophthalmol. Visual Sci. 32, 625–632.

    CAS  Google Scholar 

  42. Glonek, T., Greiner, J.V., and Lass, J.H. (1990) inNMR: Principles and Applications to Biomedical Research (Pettegrew, J.W., ed.) pp. 157–203, Springer-Verlag, New York.

    Google Scholar 

  43. Meneses, P., Greiner, J.V., and Glonek, T. (1990)Exp. Eye Res. 50, 235–240.

    Article  PubMed  CAS  Google Scholar 

  44. Merchant, T.E., Lass, J.H., Roat, M.I., Skelnik, D.L., and Glonek, T. (1990)Curr. Eye Res. 9, 1167–1176.

    PubMed  CAS  Google Scholar 

  45. Merchant, T.E., Lass, J.H., Meneses, P., Greiner, J.V., and Glonek, T. (1991)Invest. Ophthalmol. Visual Sci. 32, 549–555.

    CAS  Google Scholar 

  46. Merchant, T.E., Kasimos, J.N., de Graaf, P.W., Minsky, B.D., Gierke, L.W., and Glonek, T. (1991)Int. J. Colorect. Dis. 6, 121–126.

    Article  CAS  Google Scholar 

  47. Merchant, T.E., Meneses, P., Gierke, L.W., Den Otter, W., and Glonek, T. (1991)Br. J. Cancer 63, 693–698.

    PubMed  CAS  Google Scholar 

  48. Merchant, T.E., de Graaf, P.W., Minsky, B.D., Obertop, H., and Glonek, T. (1993)NMR Biomed. 6, 187–193.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Radiation Oncology, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, 10021, New York, NY

    Luis Seijo, Thomas E. Merchant & Bruce D. Minsky

  2. Department of Pathology, Academic Hospital Utrecht, Utrecht, The Netherlands

    Thomas E. Merchant & Leo T. M. van der Ven

  3. Magnetic Resonance Laboratory, Midwestern University, 60615, Chicago, Illinois

    Thomas Glonek

Authors
  1. Luis Seijo
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Thomas E. Merchant
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Leo T. M. van der Ven
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Bruce D. Minsky
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Thomas Glonek
    View author publications

    You can also search for this author in PubMed Google Scholar

About this article

Cite this article

Seijo, L., Merchant, T.E., van der Ven, L.T.M. et al. Meningioma phospholipid profiles measured by31P nuclear magnetic resonance spectroscopy. Lipids 29, 359–364 (1994). https://doi.org/10.1007/BF02537190

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02537190

Keywords

Search

Navigation