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The influence of vitamin E and selenium on lipid peroxidation and aldehyde dehydrogenase activity in rat liver and tissue

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Lipids

Abstract

Malondialdehyde (MDA) production and cytosolic aldehyde dehydrogenase (ALDH) response were examined in rat liver tissues after feeding different levels of dietary vitamin E and/or selenium and polyunsaturated fat for 12–38 wk. MDA production was significantly increased by vitamin E deficiency or by high levels of polyunsaturated fat intake, but not by selenium deficiency. The activity of cytosolic ALDH increased upon increased production of MDA after 12–16 wk of feeding the lipid peroxidation-inducing diets. However, ALDH activity was suppressed after 38 wk of feeding the vitamin E-deficient diet. The results indicate that the hepatic cytosolic ALDH may be involved in the metabolism of MDA during a relatively short-term increase inin vivo lipid peroxidation, but that ALDH activity becomes suppressed after more severein vivo lipid peroxidation has been produced. Hepatic and plasma α-tocopherol levels and lipid peroxidation products were measured for the various dietary groups.

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Abbreviations

ALDH:

aldehyde dehydrogenase

−E:

basal vitamin E-deficient diet

HP:

high PUFA diet

LP:

low PUFA diet

MDA:

malondialdehyde

MP:

medium PUFA diet

NE:

diet supplemented with 30 ppm vitamin E

PUFA:

polyunsaturated fatty acids

Se:

selenium

−Se−E:

Se-and vitamin E-deficient basal diet

−Se+E:

diet supplemented with 30 ppm vitamin E asRRR-α-tocopheryl acetate for the Se-deficient group

+Se-E:

diet supplemented with 0.2 ppm Se as sodium selenite for the vitamin E-deficient group

+Se+E:

diet supplemented with 30 ppm vitamin E asRRR-α-tocopheryl acetate and 0.2 ppm Se

TBA:

thiobarbituric acid

TBARS:

thiobarbituric acid reactive substances

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Author notes

  1. Hye-Sung Lee

    Present address: Department of Food Science and Nutrition, College of Human Ecology, Kyungpook National University, 702-70, Taegu, South Korea

Authors and Affiliations

  1. Department of Food Science and Nutrition, University of Minnesota, 55108, St. Paul, Minnesota

    Hye-Sung Lee & A. Saari Csallany

Authors
  1. Hye-Sung Lee
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  2. A. Saari Csallany
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Lee, HS., Csallany, A.S. The influence of vitamin E and selenium on lipid peroxidation and aldehyde dehydrogenase activity in rat liver and tissue. Lipids 29, 345–350 (1994). https://doi.org/10.1007/BF02537188

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  • DOI: https://doi.org/10.1007/BF02537188

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