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Lipoprotein lipase and hepatic lipase in Wistar and Sprague-Dawley rat tissues. Differences in the effects of gender and fasting

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Lipids

Abstract

To evaluate the effects of strain, gender and fasting in the regulation of lipoprotein lipase (LPL) and hepatic lipase (HL) activities were measured in tissues of male and female Wistar and Sprague-Dawley rats after feeding or a 24-h starvation period. It is noteworthy that an effect of gender on LPL activity was observed in Wistar, but not in Sprague-Dawley rats, not in the basal (fed) activity in several tissues, such as white and brown adipose tissues, heart, and brain, but also in response to fasting which affected LPL activity in brown adipose tissue, heat and lung of female but not of male Wistar rats. By contrast, HL activity in liver, plasma and adrenals of Sprague-Dawley rats was higher in females than in males. No effect of gender on HL activity was observed in Wistar rats. Our results indicate that differences exist between Wistar and Sprague-Dawley rats in the regulation of both LPL and HL. Some of the contradictory results found in the literature may be explained by the differences between rat strains and gender, as well as differences in the nutritional status of the animals.

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Abbreviations

BAT:

brown adipose tissue

HDL:

high density lipoproteins

Hepes:

N-(2-hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)

HL:

hepatic lipase

LPL:

lipoprotein lipase

Pipes:

piperazine-N,N′-bis(2-ethanesulfonic acid)

Tris:

tris(hydroxymethyl)aminomethane

WAT:

white adipose tissue

References

  1. Nilsson-Ehle, P., Garfinkel, A.S., and Schotz, M.C. (1980)Ann. Rev. Biochem. 49, 667–693.

    Article  PubMed  CAS  Google Scholar 

  2. Cryer, A. (1981)Int. J. Biochem. 13, 525–541.

    Article  PubMed  CAS  Google Scholar 

  3. Ramírez, I., Llobera, M., and Herrera, E. (1983)Metabolism 32, 333–341.

    Article  PubMed  Google Scholar 

  4. Vilaró, S., Llobera, M., Bengtsson-Olivecrona, G., and Olivecrona, T. (1988)Biochem. J. 249, 549–556.

    PubMed  Google Scholar 

  5. Burgaya, F., Peinado, J., Vilaró, S., Llobera, M., and Ramírez, I. (1989)Biochem. J. 259, 159–166.

    PubMed  CAS  Google Scholar 

  6. Camps, L., Reina, M., Llobera, M., Vilaró, S., and Olivecrona, T. (1990)Am. J. Physiol. 258, C673-C681.

    PubMed  CAS  Google Scholar 

  7. Landin, B., Nilsson, A., Twu, J.S., and Schotz, M.C. (1984)J. Lipid Res. 25, 559–563.

    PubMed  CAS  Google Scholar 

  8. Simard, G., and Perret, B. (1990)Ann. Biol. Clin. 48, 61–76.

    CAS  Google Scholar 

  9. Vilaró, S., Ramírez, I., Bengtsson-Olivecrona, G., Olivecrona, T., and Llobera, M. (1988)Biochim. Biophys. Acta 959, 106–117.

    PubMed  Google Scholar 

  10. Laposata, E.A., Laboda, H.M., and Strauss III, J.F. (1987)J. Biol. Chem. 262, 5333–5338.

    PubMed  CAS  Google Scholar 

  11. Jansen, H., Kalman, C., Birkenäger, J.C., and Hüsmann, W.C. (1980)FEBS Lett. 112, 30–34.

    Article  PubMed  CAS  Google Scholar 

  12. Jansen, H., and De Greef, W.J. (1981)Biochem. J. 196, 739–745.

    PubMed  CAS  Google Scholar 

  13. Doolittle, M.H., Wong, H., Davis, R.C., and Schotz, M.C. (1987)J. Lipid Res. 28, 1326–1334.

    PubMed  CAS  Google Scholar 

  14. Hixenbaugh, E.A., Sullivan, Jr., T.R., Straus III, J.F., Laposata, E.A., Komaromy, M., and Paavola, L.G. (1989)J. Biol. Chem. 264, 4222–4230.

    PubMed  CAS  Google Scholar 

  15. Peterson, J., Olivecrona, T., and Bengtsson-Olivecrona, G. (1985)Biochim. Biophys. Acta 837, 262–270.

    PubMed  CAS  Google Scholar 

  16. Glaser, D.S., Yost, T.J., and Eckel, R.H. (1992)J. Lipid Res. 33, 209–214.

    PubMed  CAS  Google Scholar 

  17. Eckel, R.H. (1987) inLipoprotein Lipase (Borensztajn, J., ed.) pp. 79–132, Evener, Chicago.

    Google Scholar 

  18. Borensztajn, J. (1987) inLipoprotein Lipase (Borensztajn, J., ed.) pp. 133–148, Evener, Chicago.

    Google Scholar 

  19. Cherkes, A., and Gordon, Jr., R.S. (1959)J. Lipid Res. 1, 97–101.

    CAS  Google Scholar 

  20. Robinson, D.S. (1965)J. Lipid Res. 6, 222–227.

    PubMed  CAS  Google Scholar 

  21. Tan, M.H., Sata, T., and Havel, R.J. (1977)J. Lipid Res. 18, 363–370.

    PubMed  CAS  Google Scholar 

  22. Grinberg, D.R., Ramírez, I., Vilaró, S., Reina, M., Llobera, M., and Herrera, E. (1985)Biochim. Biophys. Acta 833, 217–222.

    PubMed  CAS  Google Scholar 

  23. Galan, X., Llobera, M., and Ramírez, I. (1993)Biol. Neonate. 64, 295–303.

    PubMed  CAS  Google Scholar 

  24. Ramírez, I., Kryski, A.J., Ben-Zeev, O., Schotz, M.C., and Severson, D.L. (1985)Biochem. J. 232, 229–236.

    PubMed  Google Scholar 

  25. Vilaró, S., Reina, M., Ramírez, I., and Llobera, M. (1986)Biochem. J. 236, 273–278.

    PubMed  Google Scholar 

  26. Burgaya, F., Peinado, J., Llobera, M., and Ramírez, I. (1989)Biosci. Rep. 9, 559–564.

    Article  PubMed  CAS  Google Scholar 

  27. Wilson, D.E., Flowers, C.M., Carlile, S.I., and Udall, K.S. (1976)Atherosclerosis 24, 491–499.

    Article  PubMed  CAS  Google Scholar 

  28. Peinado-Onsurbe, J., Soler, C., Galan, X., Poveda, B., Soley, M., Llobera, M., and Ramírez, I. (1991)Endocrinology 129, 2599–2606.

    Article  PubMed  CAS  Google Scholar 

  29. Nikkilä, E.A., Taskinen, M.R., Rehunen, S., and Harkonen, M. (1978)Metabolism 27, 1661–1670.

    Article  PubMed  Google Scholar 

  30. Taskinen, M.R., and Nikkilä, E.A. (1981)Metabolism 30, 810–817.

    Article  PubMed  CAS  Google Scholar 

  31. Taskinen, M.R., Nikkilä, E.A., Kuusi, T., and Harno, K. (1982)Diabetologia 22, 46–50.

    Article  PubMed  CAS  Google Scholar 

  32. Henderson, A.D., Richmond, W., and Elkeles, R.S. (1993)Clin. Chem. 39, 218–223.

    PubMed  CAS  Google Scholar 

  33. Hamosh, M., and Hamosh, P. (1975)J. Clin. Invest. 55, 1132–1135.

    PubMed  CAS  Google Scholar 

  34. Cryer, A., and Jones, H.M. (1979)Comp. Biochem. Physiol. 63B, 501–505.

    CAS  Google Scholar 

  35. Ramírez, I. (1981)Am. J. Physiol. 240, E533-E538.

    PubMed  Google Scholar 

  36. Malendowicz, L.K., and Paluszak, J. (1984)Horm. Metab. Res. 16, 646–649.

    Article  PubMed  CAS  Google Scholar 

  37. Staels, B., Jansen, H., van Tol, A., Stahnke, G., Will, H., Verhoeven, G., and Auwerx, J. (1990)J. Lipid Res. 31, 1211–1218.

    PubMed  CAS  Google Scholar 

  38. Tikkanen, M.J., and Nikkilä, E.A. (1987)Am. Heart J. 113, 562–567.

    Article  PubMed  CAS  Google Scholar 

  39. Applebaum, D.M., Goldberg, A.P., Pykälistö, O.J., Brunzell, J.D., and Hazzard, W.R. (1977)J. Clin. Invest. 59, 601–608.

    PubMed  CAS  Google Scholar 

  40. Applebaum-Bowden, D., McLean, D., Steinmetz, A., Fontana, D., Matthys, C., Warnick, G.R., Cheung, M., Albers, J.J., and Hazzard, W.R. (1989)J. Lipid Res. 30, 1895–1906.

    PubMed  CAS  Google Scholar 

  41. Tikkanen, M.J., Kuusi, T., Nikkilä, E.A., and Stenman, U.H. (1986)Metabolism 35, 99–104.

    Article  PubMed  CAS  Google Scholar 

  42. Huttunen, J.K., Ehnholm, C., Kekki, M., and Nikkilä, E.A. (1976)Clin. Sci. Mol. Med. 50, 249–260.

    PubMed  CAS  Google Scholar 

  43. Eckel, R.H., and Robbins, R.J. (1984)Proc. Natl. Acad. Sci. USA 81, 7604–7607.

    Article  PubMed  CAS  Google Scholar 

  44. Gavin, L.A., Cavalieri, R.R., Moeller, M., McMahon, F.A., Castle, J.N., and Gulli, R. (1987)Metabolism 36, 919–924.

    Article  PubMed  CAS  Google Scholar 

  45. Hamosh, M., and Hamosh, P. (1975)Biochim. Biophys. Acta 380, 132–140.

    PubMed  CAS  Google Scholar 

  46. Fried, S.K., Hill, J.O., Nickel, M., and Digirolamo, M. (1983)J. Nutr. 113, 1861–1869.

    PubMed  CAS  Google Scholar 

  47. Studer, R.K., and Borle, A.B. (1982)J. Biol. Chem. 257, 7987–7993.

    PubMed  CAS  Google Scholar 

  48. Studer, R.K., and Ganas, L. (1988)Biochim. Biophys. Acta 969, 78–85.

    Article  PubMed  CAS  Google Scholar 

  49. Guerre-Millo, M., Leturque, A., Girard, J., and Lavau, M. (1985)J. Clin. Invest. 76, 109–116.

    Article  PubMed  CAS  Google Scholar 

  50. Okamura, T., and Saito, K. (1190)Biochim. Biophys. Acta 1051, 300–305.

    Google Scholar 

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Authors and Affiliations

  1. Unitat de Bioquímica i Biologia Molecular B. Departament de Bioquímica i Fisiologia, Facultat de Biologia, Universitat de Barcelona, 08071, Diagonal 645, Barcelona, Spain

    Xavier Galan, Miquel Llobera & Ignasi Ramírez

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  1. Xavier Galan
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  2. Miquel Llobera
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  3. Ignasi Ramírez
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Galan, X., Llobera, M. & Ramírez, I. Lipoprotein lipase and hepatic lipase in Wistar and Sprague-Dawley rat tissues. Differences in the effects of gender and fasting. Lipids 29, 333–336 (1994). https://doi.org/10.1007/BF02537186

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  • DOI: https://doi.org/10.1007/BF02537186

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