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Differential modulation of cytosolic lipases activities in liver and adipose tissue by high-carbohydrate diets

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Abstract

Several studies have demonstrated that a high-fructose (FRUC) diet induces metabolic and haemodynamic abnormalities, known as the metabolic syndrome, which are characterised by obesity, glucose intolerance, insulin resistance, dyslipidaemia and hypertension. In this study, the effect of a FRUC diet (60 % fructose) for 8 weeks on the metabolism of lipids in liver and epididymal adipose tissue from Wistar rats was compared with the AIN-93M diet and the effects of the AIN-93M diet were compared with a chow diet. The FRUC diet induced marked increases in both hepatocyte lipid droplet volume and postprandial serum levels of triacylglycerol (TAG), but reduced the postprandial serum levels of insulin. The AIN-93M diet induced marked increases in the hepatocyte lipid droplet volume and the serum levels of insulin, without affecting the serum levels of TAG. We found that isocaloric substitution of cornstarch, dextrinised cornstarch and sucrose (AIN-93M diet) for fructose did not affect the hepatic VLDL-TAG secretion and adipose tissue glucose uptake, lipolysis and cytosolic lipases activities in rats. However, the high-fructose diet induced a severe steatosis in liver accompanied by a decrease in cytosolic lipases activities. In adipose tissue, the FRUC diet induced a decrease in the lipoprotein lipase activity, and an increase in lipogenesis. FRUC and AIN-93M diets induced changes in lipid homeostasis in liver and adipose tissue by distinct biochemical mechanisms.

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Acknowledgments

We thank to the fellowship students of Undergraduate Research Program from Federal University of São João del-Rei, M.T.M. Lacerda and A.B. Bilheiro, for their help in determining the rate of the VLDL-TAG secretion and the liver glycogen concentration, respectively. This study was supported by Fundação de Amparo à Pesquisa de Minas Gerais (APQ-00299-12) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (471544/2012-4). A.H.R. received a fellowship from the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). C.C.L.M., E.G.M. and L.M.S.C. received a fellowship from the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).

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  1. Department of Nutrition, Federal University of Juiz de Fora, Governador Valadares, Minas Gerais, Brazil

    Angélica Heringer Rodrigues

  2. Department of Physiology and Biophysics, Biological Sciences Institute, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil

    Carolina Campos Lima Moreira, Érica Guilhen Mario, Letícia Maria de Souza Cordeiro & Leida Maria Botion

  3. Department of Morphology, Biological Sciences Institute, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil

    Gleide Fernandes Avelar

  4. Laboratory of Physiology, Federal University of São João del-Rei, Avenida Sebastião Gonçalves Coelho, 400, Chanadour, Divinópolis, Minas Gerais, 35501-296, Brazil

    Valéria Ernestânia Chaves

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  1. Angélica Heringer Rodrigues
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Rodrigues, A.H., Moreira, C.C.L., Mario, É.G. et al. Differential modulation of cytosolic lipases activities in liver and adipose tissue by high-carbohydrate diets. Endocrine 53, 423–432 (2016). https://doi.org/10.1007/s12020-016-0886-9

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