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Presence of platelet-activating factor in pyuria in humans

  • PAF and the Renal System
  • Published:
Lipids

Abstract

The relationship between the occurrence of platelet-activating factor (PAF) and neutrophils in urine from patients with urinary tract infection was examined. PAF was detected in human pyuria, when leukocyte levels reached at least 300 cells/μL (n=45), but not in normal urine (n=12). The amount of PAF found in pyuria, measured by platelet aggregation assay, was 0.01 to 13.3 pmol/mL. A close correlation was seen between the amount of PAF present and the number of urinary leukocytes (p<0.01, r=0.70). The leukocytes in pyuria consisted almost entirely of neutrophils (96±4%, mean ±S.D.). Our findings suggest that the occurrence of PAF is associated with the accumulation of neutrophils in urine.

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Abbreviations

BSA:

bovine serum albumin

C16PAF:

1-O-hexadecyl-2-acetyl-sn-glycero-3-phosphocholine

PAF:

platelet-activating factor

Rf :

migration rate

TLC:

thin-layer chromatography

References

  1. Braquet, P., Touqui, L., Shen, T.Y., and Vargaftig, B.B. (1987)Pharmacol. Rev. 39, 97–145.

    PubMed  CAS  Google Scholar 

  2. Pinckard, R.N., Ludwig, J.C., and McManus, L.M. (1988) inInflammation: Basic Principles and Clinical Correlates (Gallin, J.I., Goldstein, I.M., and Snyderman, R., eds.) pp. 139–167, Raven Press, New York.

    Google Scholar 

  3. Lotner, G.Z., Lynch, J.M., Betz, S.J., and Henson, P.M. (1980)J. Immunol. 124, 676–684.

    PubMed  CAS  Google Scholar 

  4. Shaw, J.O., Pinckard, R.N., Kathleen, K.S., McManus, L.M., and Hanahan, D.J. (1981)J. Immunol. 127, 1250–1255.

    PubMed  CAS  Google Scholar 

  5. Sanchez-Crespo, M., Inarrea, P., Alvarez, V., Alonso, F., Egido, J., and Hernando, L. (1983)Am. J. Physiol. 244, F706-F711.

    PubMed  CAS  Google Scholar 

  6. Terashita, Z., Tsushima, S., Yoshioka, Y., Nomura, H., Inada, Y., and Nishikawa, K. (1983)Life Sci. 32, 1975–1982.

    Article  PubMed  CAS  Google Scholar 

  7. Terashita, Z., Imura, Y., Takatani, M., Tsushima, S., and Nishikawa, K. (1987)J. Pharmacol. Exp. Ther. 242, 263–268.

    PubMed  CAS  Google Scholar 

  8. Okamoto, M., Yoshida, K., Nishikawa, M., Ando, T., Iwami, M., Kohsaka, M., and Aoki, H. (1986)J. Antibiot. (Tokyo) 39, 198–204.

    CAS  Google Scholar 

  9. Oda, M., Satouchi, K., Yasunaga, K., and Saito, K. (1985)J. Immunol. 134, 1090–1093.

    PubMed  CAS  Google Scholar 

  10. Bligh, E.G., and Dyer, J.A. (1959)Can. J. Physiol. 37, 911–917.

    CAS  Google Scholar 

  11. Pinckard, R.N., Farr, R.S., and Hanahan, D.J. (1979)J. Immunol. 123, 1847–1857.

    PubMed  CAS  Google Scholar 

  12. Satouchi, K., Oda, M., Saito, K., and Hanahan, D.J. (1984)Arch. Biochem. Biophys. 234, 318–321.

    Article  PubMed  CAS  Google Scholar 

  13. Satouchi, K., Oda, M., Yasunaga, K., and Saito, K. (1983)J. Biochem. (Tokyo) 94, 2067–2070.

    CAS  Google Scholar 

  14. Nakayama, R., Yasuda, K., and Saito, K. (1987)J. Biol. Chem. 262, 13174–13179.

    PubMed  CAS  Google Scholar 

  15. Mallet, A.I., and Cunningham, F.M. (1985)Biochem. Biophys. Res. Commun. 126, 192–198.

    Article  PubMed  CAS  Google Scholar 

  16. Oda, M., Satouchi, K., Ikeda, I., Sakakura, M., Yasunaga, K., and Saito, K. (1990)Am. Rev. Respir. Dis. 141, 1469–1473.

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. The First Department of Internal Medicine, Kansai Medical University, 570, Moriguchi Osaka, Japan

    Kojiro Yasunaga

  2. Department of Internal Medicine, Saiseikai Izuo Hospital, 3-4-5, Kitamura, Taisho-ku, 551, Osaka, Japan

    Ichiro Ikeda, Makoto Oda & Muneki Sakakura

Authors
  1. Ichiro Ikeda
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  2. Makoto Oda
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  3. Muneki Sakakura
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  4. Kojiro Yasunaga
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Ikeda, I., Oda, M., Sakakura, M. et al. Presence of platelet-activating factor in pyuria in humans. Lipids 26, 1333–1335 (1991). https://doi.org/10.1007/BF02536560

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  • DOI: https://doi.org/10.1007/BF02536560

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