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A specific, sensitive and high-capacity immunoassay for PAF

  • PAF Analysis by Spectrometric and Immunological Assay
  • Published:
Lipids

Abstract

A specific radioimmunoassay for platelet-activating factor (PAF) sensitive in the range 10–1000 pg (0.02–2 pmoles) has been developed. Detailed quantitative hapten inhibition studies showed specificity for the acetyl group at C-2 of PAF, a requirement for the ether linkage at C-1 and some tolerance for substituents on the choline nitrogen. No significant cross-reactivity was found with phosphatidylcholine and lysophosphatidylcholine or with lysoPAF.

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Abbreviations

Acetyl-C18GPDME:

1-O-octadecyl-2-acetyl-sn-glycero-3-phospho-N,N-dimethylethanolamine

acetyl-C18GPE:

1-O-octadecyl-2-acetyl-sn-glycerol-3-phosphoethanolamine

acetyl-C16 lysoPAF:

1-O-hexadecanoyl-2-acetyl-sn-glycero-3-phosphocholine

butyryl-C18lysoPAF:

1-O-octadecyl-2-butyryl-sn-glycero-3-phosphocholine

C16PAF:

1-O-hexadecyl-2-acetyl-sn-glycero-3-phosphocholine

(±)C16PAF:

racemic C16PAF

C18PAF:

1-O-octadecyl-2-acetyl-sn-glycero-3-phosphocholine

C18∶1PAF:

1-O-(cis-octadec-9′-enyl)-2-acetyl-sn-glycero-3-phosphocholine

DMOC12PAF:

1-O-(12′, 12′-dimethoxydodecyl)-2-acetyl-sn-glycero-3-phosphocholine

Ig:

immunoglobulin

[125I]PAF:

1-O-125I]alkyl-2-acetyl-sn-glycero-3-phosphocholine

lysoPAF:

1-O-alkyl-sn-glycero-3-phosphocholine

2-O-methyl-C18lysoPAF:

1-O-octadecyl-2-O-methyl-sn-glycero-3-phosphocholine

PAF:

platelet-activating factor

propionyl-C18lysoPAF:

1-O-octadecyl-2-propionyl-sn-glycero-3-phosphocholine

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Authors and Affiliations

  1. Kolling Institute of Medical Research, Royal North Shore Hospital of Sydney, St, 2065, Leonards, N.S.W.

    Brian A. Baldo & Mary A. Smal

  2. Department of Medicine, University of Sydney, 2006, Sydney

    Brian A. Baldo

  3. Silenus Laboratories, 3122, Victoria, Australia

    Alistair C. McCaskill

Authors
  1. Brian A. Baldo
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  2. Mary A. Smal
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  3. Alistair C. McCaskill
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Baldo, B.A., Smal, M.A. & McCaskill, A.C. A specific, sensitive and high-capacity immunoassay for PAF. Lipids 26, 1136–1139 (1991). https://doi.org/10.1007/BF02536517

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  • DOI: https://doi.org/10.1007/BF02536517

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