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Quantification of Porcine Complement Activation Fragment C3a by a Neoepitope-Based Enzyme-Linked Immunosorbent Assay

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The Complement System

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2227))

Abstract

Enzyme-linked immunosorbent assay (ELISA) enables fast and simple quantification of analytes in the pico- to nanogram range in complex samples. Here, we describe an ELISA for the detection of porcine C3a as a marker for complement activation. Antibody specificity is critical for a robust assay. This assay is based on a pair of antibodies specific for the porcine C3a molecule and thus does not react with native C3.

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Authors and Affiliations

  1. Department of Immunology, University of Oslo and Oslo University Hospital Rikshospitalet, Oslo, Norway

    Per H. Nilsson & Tom Eirik Mollnes

  2. Linnaeus Centre for Biomaterials Chemistry, Linnaeus University, Kalmar, Sweden

    Per H. Nilsson

  3. Research Laboratory, Nordland Hospital, Bodø, Norway

    Kristin Pettersen, Espen W. Skjeflo, Hilde Fure & Dorte Christiansen

  4. Institute for Cellular and Molecular Immunology, University Medical Center, Georg-August-University Göttingen, Göttingen, Germany

    Martin Oppermann

  5. Faculty of Health Sciences, K.G Jebsen TREC, UiT—The Arctic University of Norway, Tromsø, Norway

    Espen W. Skjeflo & Tom Eirik Mollnes

  6. Centre of Molecular Inflammation Research, Norwegian University of Science and Technology, Trondheim, Norway

    Tom Eirik Mollnes

Authors
  1. Per H. Nilsson
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  2. Kristin Pettersen
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  3. Martin Oppermann
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  4. Espen W. Skjeflo
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  5. Hilde Fure
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  6. Dorte Christiansen
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  7. Tom Eirik Mollnes
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Corresponding author

Correspondence to Per H. Nilsson .

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Editors and Affiliations

  1. Centre de Recherche des Cordeliers, INSERM, Sorbonne Université, USPC, Université Paris Descartes, Université Paris Diderot, Paris, France

    Lubka T. Roumenina

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Nilsson, P.H. et al. (2021). Quantification of Porcine Complement Activation Fragment C3a by a Neoepitope-Based Enzyme-Linked Immunosorbent Assay. In: Roumenina, L.T. (eds) The Complement System. Methods in Molecular Biology, vol 2227. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1016-9_5

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  • DOI: https://doi.org/10.1007/978-1-0716-1016-9_5

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1015-2

  • Online ISBN: 978-1-0716-1016-9

  • eBook Packages: Springer Protocols

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