Abstract
The effects of pyrimido-pyrimidine derivatives (dipyridamole, RA-642, and RA-233) on lipid peroxidation, using d-α-tocopherol as standard, were studied in enriched membrane fractions from human and rat hepatocytes. Equimolar concentrations of ferrous sulfate and ascorbic acid were used to induce lipid peroxidation. The amount of peroxidized lipids observed in membrane fractions from human liver was smaller than in those from rat liver. In both species, however, pyrimido-pyrimidine derivatives, except for RA-233 in rat liver, inhibited lipid peroxidation dose-dependently in the following sequence: RA-642 > dipyridamole > d-α-tocopherol RA-233.
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Abbreviations
- Cyt C:
-
cytochrome C
- ED-50:
-
effective concentration 50%
- FeAs:
-
ferrous sulfate/ascorbic acid
- GMN:
-
glomerulonephritis
- HA:
-
hydroxyl anions
- IC-50:
-
inhibitory concentration, 50%
- MDA:
-
malondialdehyde
- NBT:
-
nitroblue tetrazolium
- ODFR:
-
oxygen-derived free radicals
- PTRA:
-
post-thrombolysis re-perfusion arrhythmias
References
Kogure, K., Watson, R., Busto, R., and Abe, K. (1982)Neurochem. Res. 7, 437–454.
Emerit, J., Fechner, J., Galli, A., Clavel, J.P., and Congy, P. (1986)Presse Med. 15, 751–754.
Loven, D., Schedl, H., Wilson, H., Daabees, T., Stegink, L., Diekus, M., and Oberley, L. (1986)Diabetes 35, 503–507.
Fecondo, J.V., and Augusteyn, R.C. (1983)Exp. Eye Res. 36, 15–19.
Poli, G., Albano, E., and Dianzani, M. (1987)Chem. Phys. Lipids 45, 117–142.
Santrucek, M., and Krepelka, J. (1988)Drugs of the Future 13, 974–996.
Emmons, P., Harrison, M., Honour, A., and Mitchell, J. (1965)Lancet 2, 603.
De la Cruz, J.P., García, A., de la Torre, M., Carpintero, J.L., and Sánchez de la Cuesta, F. (1988)Rev. Pharmacol. Clin. Exp. 5, 226 (Abstr.).
Cámara, S., de la Cruz, J.P., Frutos, M.A., Sánchez, P., López, E., Sánchez, E., and Sánchez de la Cuesta, F. (1991)Nephron 58, 13–16.
Bellido, I., de la Cruz, J.P., and Sánchez de la Cuesta, F. (1991)Meth. Find. Exptl. Clin. Pharmacol. 13, 371–375.
Iuliano, L., Violi, F., Ghiselli, A., Alessandri, C., and Balsano, F. (1989)Lipids 24, 430–433.
Halliwell, G. (1990)Free Radical Res. Commun. 9, 1–32.
Lowry, O., Rosebrough, H., Farr, A., and Randall, R. (1951)J. Biol. Chem. 193, 265–275.
Wendel, A., and Feuerstein, S. (1981)Biochem. Pharmacol. 30, 2513–2520.
Bosmann, H.B., and Hemsworth, B.A. (1969)Structure and Function of Nervous Tissue, 2nd edn., pp. 1–24, Academic Press, New York.
Slater, T., Cheeseman, K., Davies, M., Hayashi, M., Sharma, O., Nigam, S., and Benedetto, C. (1987)Adv. Prostaglandin Thromboxane Leukotriene Res. 17, 1098–1102.
Keppler, L., and Novacky, A. (1987)Physiol. Mol. Plant Pathol. 30, 233–245.
De la Cruz, J.P., Moreno, A., Sánchez de la Cuesta, F., and Garcia Campos, J. (1990)Can. J. Ophthalmol. 25, 329–332.
De la Cruz, J.P., Sintas, A., Moreno, A., Garcia Campos, J., and Sánchez de la Cuesta, F. (1991)Pharmacol. and Toxicol. 69, 201–204.
Reuter, H. (1971)Acta Med. Scan. 190, 239–245.
De la Cruz, J.P., Moron, D., and Sanchez de la Cuesta, F. (1991)Thromb. Res. 63, 463–468.
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de la Cruz, J.P., Carrasco, T., Ortega, G. et al. Inhibition of ferrous-induced lipid peroxidation by pyrimido-pyrimidine derivatives in human liver membranes. Lipids 27, 192–194 (1992). https://doi.org/10.1007/BF02536177
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DOI: https://doi.org/10.1007/BF02536177