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Different fatty chain compositions of alkenylacyl, alkylacyl and diacyl phospholipids in rabbit alveolar macrophages: High amounts of arachidonic acid in ether phospholipids

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Lipids

Abstract

High levels of ether phospholipids were found in rabbit alveolar macrophages. Choline phosphoglycerides (CPG) contained a significant amount of alkylacyl compound (32.5%). On the other hand, ethanolamine phosphoglyceride (EPG) included a very large amount of alkenylacyl compounds (61.2%). Small amounts of alkenylacyl CPG and alkylacyl EPG were also observed. The occurrence of a high amount of alkylacyl CPG may be related to the synthesis or release of platelet-activating factor (PAF) from macrophages. Fatty chains at the 1- and 2-positions in each lipid class of CPG or CPG or alkenylacyl EPG were several other. Particularly, the levels of 20∶4 (arachidonic acid) in alkylacyl CPG or alkenylacyl EPG were several times higher than those in corresponding diacyl phospholipids. Large portions of 20∶4-containing species have alkenyl or alkyl ether moieties at their 1-position in both CPG (73.6%) and EPG (85.9%). These results suggest the importance of ether-containing phospholipids in rabbit alveolar macrophages.

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Abbreviations

CPG:

choline phosphoglycerides

EPG:

ethanolamine phosphoglycerides

PAF:

platelet-activating factor

Hepes:

N-2-hydioxyethylpiperazine-N′-2-ethanesulphonic acid

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Authors and Affiliations

  1. Faculty of Pharmaceutical Sciences, Teikyo University, Sagamiko, Kanagawa, 19901, Japan

    Takayuki Sugiura, Motoko Nakajima, Nobuyuki Sekiguchi, Yasuhito Nakagawa & Keizo Waku

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  1. Takayuki Sugiura
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  2. Motoko Nakajima
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  3. Nobuyuki Sekiguchi
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  4. Yasuhito Nakagawa
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  5. Keizo Waku
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Sugiura, T., Nakajima, M., Sekiguchi, N. et al. Different fatty chain compositions of alkenylacyl, alkylacyl and diacyl phospholipids in rabbit alveolar macrophages: High amounts of arachidonic acid in ether phospholipids. Lipids 18, 125–129 (1983). https://doi.org/10.1007/BF02536106

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  • DOI: https://doi.org/10.1007/BF02536106

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