Skip to main content
SpringerLink
Log in

Quantitation of 1,2-diacylglycerol in rat heart by latroscan TLC/FID

  • Methods
  • Published:
Lipids

Abstract

1,2-Diacylglycerol, which has been recognized as one of the intracellular second messengers, was measured quantitatively in the lipid extract from rat hearts using the thin layer chromatography and flame ionization detection (TLC/FID) method. Cholesterol acetate was added to the tissue as an internal standard, and the crude lipids from the tissue were purified with silicic acid column to eliminate phospholipids. Development of Chromarods was carried out using two solvent systems and a three-step development technique. The relationship of the peak area ratio detected by flame ionization detector to weight ratio was linear compared with cholesteryl acetate. The 1,2-diacylglycerol content in the rat heart in the unstimulated condition was 72.5±15.3 ng/mg wet wt (mean±SD).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

DG:

diacylglycerol

FID:

flame ionization detection

HPLC:

high performance liquid chromatography

TLC:

thin layer chromatography

References

  1. Berridge, M.J. (1984)Biochem. J. 220, 345–360.

    PubMed  CAS  Google Scholar 

  2. Nishizuka, Y. (1984)Science 225, 1365–1370.

    Article  PubMed  CAS  Google Scholar 

  3. Banschbach, M.W., Geison, R.L., and O'Brien, J.F. (1974)Anal. Biochem. 59, 617–627.

    Article  PubMed  CAS  Google Scholar 

  4. Freeman, C.P., and West, D. (1966)J. Lipid Res. 7, 324–327.

    PubMed  CAS  Google Scholar 

  5. Satouchi, K., and Saito, K. (1976)Biomed. Mass Spectrom. 3, 122–126.

    Article  PubMed  CAS  Google Scholar 

  6. Abe, K., and Kogure, K. (1986)J. Neurochem. 47, 577–582.

    Article  PubMed  CAS  Google Scholar 

  7. Folch, J., Lees, M., and Sloane-Stanley, G.H. (1957)J. Biol. Chem. 226, 497–509.

    PubMed  CAS  Google Scholar 

  8. Besterman, J.M., Duronio, V., and Cuatrecasas, P. (1986)Proc. Natl. Acad. Sci. USA 83, 6785–6789.

    Article  PubMed  CAS  Google Scholar 

  9. Bocckino, S.B., Blackmore, P.F., and Exton, J.H. (1985)J. Biol. Chem. 260, 14201–14207.

    PubMed  CAS  Google Scholar 

  10. Kramer, J.K.G. (1980)Lipids 15, 651–660.

    Article  PubMed  CAS  Google Scholar 

  11. Okumura, K., Ogawa, K., and Satake, T. (1983)J. Cardiovasc. Pharmacol. 5, 983–988.

    Article  PubMed  CAS  Google Scholar 

  12. Christie, W.W., and Hunter, M.L. (1979)J. Chromatogr. 171, 517–518.

    Article  CAS  Google Scholar 

  13. Porcellati, G., DeMeddio, G.E., Fini, C., Floridi, A., Goracci, G., and Horrocks, L.A. (1978)Proc. Eur. Soc. Neurochem. 1, 285–302.

    CAS  Google Scholar 

  14. Kramer, J.K.G., and Hulan, H.W. (1978)J. Lipid Res. 19, 103–106.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. the Second Department of Internal Medicine, Nagoya University School of Medicine, 65 Tsuruma-cho, Showa-ku, 466, Nagoya, Japan

    Kenji Okumura, Hidekazu Hashimoto, Takayuki Ito, Kouichi Ogawa & Tatsuo Satake

Authors
  1. Kenji Okumura
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hidekazu Hashimoto
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Takayuki Ito
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Kouichi Ogawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Tatsuo Satake
    View author publications

    You can also search for this author in PubMed Google Scholar

About this article

Cite this article

Okumura, K., Hashimoto, H., Ito, T. et al. Quantitation of 1,2-diacylglycerol in rat heart by latroscan TLC/FID. Lipids 23, 253–255 (1988). https://doi.org/10.1007/BF02535467

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02535467

Keywords

Search

Navigation