Abstract
Cholesterol is an essential lipid molecule for several biological functions including the proper functioning of cell membranes, lipoproteins, and lipid rafts, as well as the synthesis of bile acids, vitamin D, and steroid hormones. Cholesterol can be extracted from liver tissue by multiple methods of lipid extraction. Subsequently, gas chromatography–mass spectrometry (GC–MS) can be used to obtain the highest level of sensitivity and selectivity in the analysis of cholesterol. This chapter describes two methods of lipid extraction for liver tissue, Bligh and Dyer and methyl tertiary butyl ether (MTBE), followed by an analysis with GC–MS.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Li L, Dutkiewicz EP, Huang Y et al (2019) Analytical methods for cholesterol quantification. Yàowu fenxi 27(2):375–386. https://doi.org/10.1016/j.jfda.2018.09.001
Schade DS, Shey L, Eaton RP (2020) Cholesterol review: a metabolically important molecule. Endocr Pract 26(12):1514–1523. https://doi.org/10.4158/EP-2020-0347
Beale DJ, Pinu FR, Kouremenos KA et al (2018) Review of recent developments in GC–MS approaches to metabolomics-based research. Metabolomics 14(11):1–31. https://doi.org/10.1007/s11306-018-1449-2
Dutta M, Cai J, Gui W et al (2019) A review of analytical platforms for accurate bile acid measurement. Anal Bioanal Chem 411(19):4541–4549. https://doi.org/10.1007/s00216-019-01890-3
Sparkman OD, Penton Z, Kitson FG (2011) Gas chromatography and mass spectrometry, 2nd edn. Elsevier Science, San Diego
Sundermann A, Eggers LF, Schwudke D (2016) Liquid extraction: Bligh and dyer. Springer, Dordrecht
Eggers LF, Dominik S (2016) Lipid extraction: basics of the methyl-tert-butyl ether extraction. Springer, Dordrecht
Ostermann AI, Müller M, Willenberg I et al (2014) Determining the fatty acid composition in plasma and tissues as fatty acid methyl esters using gas chromatography – a comparison of different derivatization and extraction procedures. Prostaglandins Leukot Essent Fat Acids 91(6):235–241. https://doi.org/10.1016/j.plefa.2014.10.002
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Saleem, M.A., Benitez, B., Yaros, C., Yamar, G., Bhattacharya, S.K. (2023). Analysis of Cholesterol from the Liver Using Gas Chromatography–Mass Spectrometry. In: González-Domínguez, R. (eds) Mass Spectrometry for Metabolomics. Methods in Molecular Biology, vol 2571. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2699-3_14
Download citation
DOI: https://doi.org/10.1007/978-1-0716-2699-3_14
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-2698-6
Online ISBN: 978-1-0716-2699-3
eBook Packages: Springer Protocols