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Quantitative estimation of C-24 epimeric sterol mixtures by 220 MHz nuclear magnetic resonance spectroscopy

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Lipids

Abstract

Four pairs of 24-alkyl epimeric sterols: 24α- and 24β-methylcholesterol; 24α- and 24β-ethylcholesterol; 24α- and 24β-methyl-5, 22-E-cholestadienol; and 24α- and 24β-ethyl-5, 22-E-cholestadienol, were mixed in known proportions (0, 20, 40, 50, 60, 80 and 100% of β-epimer to α-epimer). The 220 MHz nuclear magnetic resonance spectra of each of the mixtures were obtained and compared. Areas of these spectra useful in quantitative determinations were identified and discussed.

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Authors and Affiliations

  1. Department of Botany, University of Maryland, 20742, College Park, MD

    P. -L. Chiu & G. W. Patterson

Authors
  1. P. -L. Chiu
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  2. G. W. Patterson
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Chiu, P.L., Patterson, G.W. Quantitative estimation of C-24 epimeric sterol mixtures by 220 MHz nuclear magnetic resonance spectroscopy. Lipids 16, 203–206 (1981). https://doi.org/10.1007/BF02535440

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  • DOI: https://doi.org/10.1007/BF02535440

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