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A modification of the thiobarbituric acid reaction

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Lipids

Abstract

A simple modification of the thiobarbituric acid reaction, which removes nonspecific chromogenic reaction products, involves incubation at room temperature for 16–20 hr and a column chromatographic step. The chromogen-concentrating capacity of the latter step is useful for determination of low concentrations of thiobarbituric acid-reactive materials in biological preparations.

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Abbreviations

TBA:

2-thiobarbituric acid

MDA:

malonaldehyde, malondialdehyde, malonyldialdehyde

TBARM:

thiobarbituric acidreactive material

MDAMe4 :

malonaldehyde bis(dimethyl acetal), 1,1,3,3-tetramethoxy propane, tetramethoxy malonaldehyde

DIW:

deionized water

D:

difference between optical densities at 532 nm and 600 nm

OD:

optical density

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Authors and Affiliations

  1. UCLA/VA Medical Center, (116A-3), 16111 Plummer Street, 91343, Sepulveda, California

    T. George Bidder & Bona M. Sipka

Authors
  1. T. George Bidder
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  2. Bona M. Sipka
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Bidder, T.G., Sipka, B.M. A modification of the thiobarbituric acid reaction. Lipids 24, 656–658 (1989). https://doi.org/10.1007/BF02535084

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  • DOI: https://doi.org/10.1007/BF02535084

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