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Complete separation of phospholipids from human heart combining two HPLC methods

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Lipids

Abstract

The separation of phospholipid classes from human heart was achieved in two steps by high performance liquid chromatography (HPLC) using a silica column with an ultraviolet spectromonitor at 206 nm. A complete partitioning of phosphatidylcholines (PC), phosphatidylethanolamines (PE), phosphatidylinositols (PI), phosphatidylserines (PS), cardiolipins (CL), lysophosphatidylcholines (LPC) and sphingomyelins (Sph) was obtained for further analysis.

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Authors and Affiliations

  1. Station de Recherches sur la Qualité des Aliments de l'Homme, Institut National de la Recherche Agronomique, BV 1540, 21034, Dijon Cedex, France

    Pierre Juanéda & Gérard Rocquelin

Authors
  1. Pierre Juanéda
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  2. Gérard Rocquelin
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Juanéda, P., Rocquelin, G. Complete separation of phospholipids from human heart combining two HPLC methods. Lipids 21, 239–240 (1986). https://doi.org/10.1007/BF02534829

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  • DOI: https://doi.org/10.1007/BF02534829

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