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Lipid composition of liver mitochondria and microsomes in hyperthyroid rats

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Lipids

Abstract

Triiodothyronine-induced alteration of the lipid pattern in rat-liver mitochondria and microsomes has been investigated. In mitochondria, a 25% total cholesterol decrease and a 14% phospholipid increase have been detected. In these hyperthyroid rat liver organelles, a strong decrease in the total cholesterol/phospholipid molar ratio occurs. On the contrary, in microsomes from the same animals, a decrease of about 23% has been measured for both total cholesterol and phospholipids; hence, in this fraction, the total cholesterol/phospholipid molar ratio is unaffected by hyperthyroidism. The liver mitochondrial phospholipid composition, unlike the microsomal composition, is altered significantly in hyperthyroid rats; a 7.4% phosphatidylcholine decrease is accompanied by a similar additive percentage increase of both phosphatidylethanolamine and cardiolipin. In regard to total phospholipid fatty acid composition in liver microsomes from hyperthyroid rats, no variation has been observed compared with the control rats, whereas in mitochondria from the same animals, a meaningful linoleic acid decrease with a similar arachidonic acid increase has been found. In addition to fatty acid alteration, the separated mitochondrial phospholipid classes also exhibit some increase in stearic acid. Among phospholipids, cardiolipin changes the most of the esterified fatty acids in hyperthyroid rat liver. In this compound, a strong increase in the percentage of both palmitic and stearic acid and a 32.4% decrease of linoleic acid have been found.

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References

  1. Van Deenen, L.L.M. (1965) in Progress in Chemistry of Fats and Other Lipids (Holman, R.T., ed.) Vol. 8, pp. 1–127, Pergamon Press, Oxford, England.

    Google Scholar 

  2. McMurchie, E.J., and Raison, J.K. (1979) Biochim. Biophys. Acta 554, 364–374.

    Article  PubMed  CAS  Google Scholar 

  3. Feo, F., Canuto, R.A., Garcea, R., and Gabriel, L. (1975) Biochim. Biophys. Acta 413, 116–134.

    Article  PubMed  CAS  Google Scholar 

  4. Borochov, H., Zahler, P., Wilbrandt, W., and Shinitzky, M. (1977) Biochim. Biophys. Acta 470, 382–388.

    Article  CAS  Google Scholar 

  5. Shiga, T., Maeda, N., Suda, T., Konk, K., and Sekiya, M. (1979) Biochim. Biophys. Acta 553, 84–95.

    Article  PubMed  CAS  Google Scholar 

  6. Wolff, E.C., and Wolff, J. (1964) in The Thyroid Gland (Pitt-Rivers, R. and Trottner, W.R., eds.) Vol. 1 pp. 232–282, Butterworths, London, England.

    Google Scholar 

  7. Babior, B.M., Creagan, S., Ingbar, S.H., and Kipnes, R.S. (1973) Proc. Natl. Acad. Sci. USA 70, 98–102.

    Article  PubMed  CAS  Google Scholar 

  8. Ismail Beigi, F., and Edelman, I.S. (1970) Proc. Natl. Acad. Sci. USA 67, 1071–1078.

    Article  CAS  Google Scholar 

  9. Gavaret, J.M., Vilkki, P., and Rall, J.E. (1979) FEBS Lett. 108, 40–44.

    Article  PubMed  CAS  Google Scholar 

  10. Hulbert, J., Augee, M.L., and Raison, J.K. (1976) Biochim. Biophys. Acta 455, 597–601.

    Article  PubMed  CAS  Google Scholar 

  11. Hulbert, J. (1978) J. Theor. Biol. 73, 81–100.

    Article  PubMed  CAS  Google Scholar 

  12. Landriscina, C., Gnoni, G.V., and Quagliariello, E. (1976) Eur. J. Biochem. 71, 135–143.

    Article  PubMed  CAS  Google Scholar 

  13. Booth, F.W., and Holloszy, J.O. (1975) Arch. Biochem. Biophys. 167, 674–677.

    Article  PubMed  CAS  Google Scholar 

  14. Gnoni, G.V., Landriscina, C., and Quagliariello, E. (1978) FEBS Lett. 94, 179–182.

    Article  PubMed  CAS  Google Scholar 

  15. Gnoni, G.V., Landriscina, C., and Quagliariello, E. (1980) Biochem. Med. 24, 336–347.

    Article  PubMed  CAS  Google Scholar 

  16. Roncari, D.A.K., and Murthy, V.K. (1975) J. Biol. Chem. 250, 4134–4138.

    PubMed  CAS  Google Scholar 

  17. Young, D.L., and Lynen, F. (1969) J. Biol. Chem. 244, 377–383.

    PubMed  CAS  Google Scholar 

  18. Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J. (1951) J. Biol. Chem. 193, 265–275.

    PubMed  CAS  Google Scholar 

  19. Duncan, I.W., Culbreth, P.H., and Burtis, C.A. (1979) J. Chromatogr. 162, 281–292.

    PubMed  CAS  Google Scholar 

  20. Bligh, E.G., and Dyer, W.J. (1959) Can. J. Biochem. Physiol. 37, 911–917.

    PubMed  CAS  Google Scholar 

  21. Nakamura, G.R. (1952) Anal. Chem. 24, 1372.

    Article  CAS  Google Scholar 

  22. Miller, R.A., Bussel, N.E., and Ricketts, C. (1978) J. Liquid Chromatogr. 3, 291–304.

    Google Scholar 

  23. Hoch, F.L., Subramanian, C., Dhopeshwarkar, G.A., and Mead, J.F. (1981) Lipids 16, 328–335.

    PubMed  CAS  Google Scholar 

  24. Clejan, S., Collipp, P.J., and Maddaiah, V.T. (1980) Arch. Biochem. Biophys. 203, 744–752.

    Article  PubMed  CAS  Google Scholar 

  25. Faas, F.H., and Carter, W.J. (1981) Biochem. J. 193, 845–852.

    PubMed  CAS  Google Scholar 

  26. Hoch, F.L., Depierre, J.W., and Ernster, L. (1980) Eur. J. Biochem. 109, 301–306.

    Article  PubMed  CAS  Google Scholar 

  27. Gnoni, G.V., Landriscina, C., Ruggiero, F.M., and Quagliariello, E. (1983) Biochim. Biophys Acta 751, 271–279.

    PubMed  CAS  Google Scholar 

  28. Landriscina, C., Megli, F.M., and Quagliariello, E. (1976) Lipids 11, 61–66.

    Article  PubMed  CAS  Google Scholar 

  29. Stakkedstad, J.A., and Bremer, J. (1982) Biochim. Biophys. Acta 711, 90–100.

    Google Scholar 

  30. Landriscina, C., Gnoni, G.V., and Quagliariello, E. (1972) Eur. J. Biochem. 29, 188–196.

    Article  PubMed  CAS  Google Scholar 

  31. Reitz, R.C., El-Sheikh, M., Lands, W.E.M., Ismail, I.A., and Gunstone, F.D. (1969) Biochim. Biophys. Acta 176, 480–490.

    PubMed  CAS  Google Scholar 

  32. Faas, F.H., and Carter, W.J. (1982) Biochem. J. 207, 29–35.

    PubMed  CAS  Google Scholar 

  33. Demel, R.A., and De Kruyff, B. (1976) Biochim. Biophys. Acta 457, 109–132.

    PubMed  CAS  Google Scholar 

  34. Cooper, R.A., Leslie, M.H., Fischkoff, S., Shinitzky, M., and Sanford, J.S. (1978) Biochemistry 17, 327–331.

    Article  PubMed  CAS  Google Scholar 

  35. Lehninger, A.L. (1959) J. Biol. Chem. 234, 2187–2195.

    PubMed  CAS  Google Scholar 

  36. Mathe, D., and Chevallier, F. (1976) Biochim. Biophys. Acta 441, 155–164.

    PubMed  CAS  Google Scholar 

  37. Ruggiero, F.M., Landriscina, C., Gnoni, G.V., and Quagliariello, E. (1983) Horm. Metab. Res., in press.

  38. Gnoni, G.V., Landriscina, C., and Quagliariello, E. (1980) FEBS Lett. 122, 37–40.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Research Unit on Mitochondria and Energy Metabolism, C.N.R., Via G. Amendola 165/A, 70126, Bari, Italy

    F. M. Ruggiero, G. V. Gnoni & E. Quagliariello

  2. Biochemistry Laboratory, Veterinary Faculty, University of Bari, Bari, Italy

    C. Landriscina

Authors
  1. F. M. Ruggiero
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  2. C. Landriscina
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  3. G. V. Gnoni
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  4. E. Quagliariello
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Ruggiero, F.M., Landriscina, C., Gnoni, G.V. et al. Lipid composition of liver mitochondria and microsomes in hyperthyroid rats. Lipids 19, 171–178 (1984). https://doi.org/10.1007/BF02534794

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  • DOI: https://doi.org/10.1007/BF02534794

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