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Raman studies of structural rearrangements induced in human plasma lipoprotein carotenoids by malondialdehyde

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Lipids

Abstract

Raman and resonance Raman spectra of plasma lipoproteins ± malondialdehyde were studied at concentrations which block the normal receptor-mediated uptake by cells. The strong resonance Raman bands at about 1010, 1162 and 1530 cm−1, due to the presence of carotenoids in the lipoproteins, are envisaged as structural probes. High resolution resonance Raman spectra of the 1500–1600 cm−1 region reveal multiple features suggesting the coexistence of several structural populations of β-carotene whose precise assignment is complex. When plasma lipoproteins are reacted with malondialdehyde, a complex change occurs in the resonance Raman banding of β-carotene in the 1500–1600 cm−1 region. Malonaldehyde (MDA) also modifies the acoustical region (70–200 cm−1 of low density lipoprotein (LDL) lipids. We suggest that malondialdehyde association with plasma lipoproteins alters the lipid structure via apoprotein or apoprotein/lipid associations.

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Authors and Affiliations

  1. Radiobiology Division, Tufts-New England Medical Center, 02111, Boston, MA

    Surendra P. Verma & Donald F. H. Wallach

  2. INSERM UNITE 58, CNRS-ERA 941, Montpellier, France

    Jean R. Philippot & Josette Sainte-Marie

  3. CNRS-LA 79 USTL, Montpellier, France

    Bernard Bonnet

  4. CTP, INSERM, Lille, France

    Yves Moschetto

Authors
  1. Surendra P. Verma
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  2. Jean R. Philippot
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  3. Bernard Bonnet
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  4. Josette Sainte-Marie
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  5. Yves Moschetto
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  6. Donald F. H. Wallach
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Verma, S.P., Philippot, J.R., Bonnet, B. et al. Raman studies of structural rearrangements induced in human plasma lipoprotein carotenoids by malondialdehyde. Lipids 20, 890–896 (1985). https://doi.org/10.1007/BF02534773

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  • DOI: https://doi.org/10.1007/BF02534773

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