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Adipocyte fatty acid mobilization in vivo: Effects of age and anatomical location

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Lipids

Abstract

The objectives of the present study were to determine if adipocyte trigly ceride fatty acid (TGFA) mobilization in vivo varied among the different adipose tissue depots and whether these rates were affected by age. In order to accomplish these objectives, two groups of rats were studied. The first group initially weighed 84±1 and the second group 333±2. Both groups were placed on a semisynthetic diet containing 6% corn oil (w/w) and 14% triundecanoin (w/w) for a period of 4 wk. Triundecanoin contains an 11-carbon (C-11) fatty acid (undecanoic acid) that was used to label the adipocyte TGFA. At the end of the 4-wk feeding period, triundecanoin was removed from the diet and replaced with an equivalent amount of corn oil. At this time and at weekly intervals for the next 4 wk, 5 rats from each age group were killed for the determination of TGFA composition in isolated dipocytes from the epididymal (Epi), perirenal (PR), subcutaneous (SC) and mesenteric (M) adipose tissue depots. When the content of C-11 was expressed as mole percent of the total fatty acids, mobilization was significantly more rapid from the PR and M depots than in the other two depots in the young rats. In the older rats mobilization was significantly slower in all depots compared to the younger group. The rates of mobilization were not different between the depots in the older animals. Since fat cell size continued to increase throughout the duration of the study, part of the decrease in C-11 content can be accounted for by dilution by newly acquired TGFA. When data were compared on the basis of the actual pmoles of C-11 per cell, rates of mobilization were not different between the depots nor was mobilization affected by age. These results emphasize again the impact the manner in which adipocyte metabolic data are expressed has on interpretation(s) when comparing adipocytes of different depots or from rats of different age.

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Authors and Affiliations

  1. Department of Physiology, Lousiana State University Medical Center, 70119, New Orleans, LA

    Arthur D. Hartman

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  1. Arthur D. Hartman
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Hartman, A.D. Adipocyte fatty acid mobilization in vivo: Effects of age and anatomical location. Lipids 20, 255–261 (1985). https://doi.org/10.1007/BF02534256

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  • DOI: https://doi.org/10.1007/BF02534256

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