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Monohydroperoxides of linoleic acid in endoplasmic lipids of rats exposed to tetrachloromethane

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Lipids

Abstract

An accurate method for the quantitative determination of hydroperoxy and hydroxy fatty acids in liver microsomes is presented which involves the use of18O-labeled internal standards. The method is employed for the determination of hydroperoxides in rat liver microsomes after aerobic incubation with Fe2+/ADP and in microsomes from animals exposed to 75 mg tetrachloromethane/kg body weight. The amounts found after artificial microsomal “lipid peroxidation” are almost two orders of magnitude larger than those in microsomes from tetrachloromethaneexposed animals.

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Abbreviations

GC/MS:

gas chromatography/mass spectroscopy

ADP:

adenosine 5′-diphosphate disodium salt

NADP:

nicotinamide adenine dinucleotide phosphate

PTFE:

polytetrafluoroethylene

HPLC:

high performance liquid chromatography

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Authors and Affiliations

  1. Institut fuer Toxikologie der Universitaet Tuebingen, Wilhelmstrasse 56, D-7400, Tuebingen 1, Federal Republic of Germany

    Hartmut Frank, Manfred Wiegand, Manfred Strecker & Dietmar Thiel

Authors
  1. Hartmut Frank
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  2. Manfred Wiegand
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  3. Manfred Strecker
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  4. Dietmar Thiel
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Frank, H., Wiegand, M., Strecker, M. et al. Monohydroperoxides of linoleic acid in endoplasmic lipids of rats exposed to tetrachloromethane. Lipids 22, 689–697 (1987). https://doi.org/10.1007/BF02533967

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  • DOI: https://doi.org/10.1007/BF02533967

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