Abstract
Opiates and opioid peptides carry out their regulatory effects mainly by inhibiting neuronal activity. At the cellular level, opioids block voltage-dependent calcium channels, activate potassium channels and inhibit adenylate cyclase, thus reducing neurotransmitter release. An increasing body of evidence indicates an additional opposite, stimulatory activity of opioids. The present review summarizes the potentiating effects of opioids on transmitter release and the possible cellular events underlying this potentiation: elevation of cytosolic calcium level (by either activating Ca2+ influx or mobilizing intracellular stores), blockage of K+ channels and stimulation of adenylate cyclase. Biochemical, pharmacological and molecular biology studies suggest several molecular mechanisms of the bimodal activity of opioids, including the coupling of opioid receptors to various GTP-binding proteins, the involvement of different subunits of these proteins, and the activation of several intracellular signal transduction pathways. Among the many experimental preparations used to study the bimodal opioid activity, the SK-N-SH neuroblastoma cell line is presented here as a suitable model for studying the complete chain of events leading from binding to receptors down to regulation of transmitter release, and for elucidating the molecular mechanism involved in the stimulatory effects of opioid agonists.
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References
Paton, W. D. M. 1957. The action of morphine and related substances on contraction and on acetylcholine output of coaxially stimulated guinea pig ileum. Br. J. Pharmac. Chemother. 12:119–127.
Schaumann, W. 1957. Inhibition by morphine of the release of acetylcholine from the intestine of the guinea pig. Br. J. Pharmac. Chemother. 12:115–118.
Beleslin, D., and Polak, R. I. 1965. Depression by morphine and chloralose of acetylcholine release from the cat's brain. J. Physiol. 117:411–419.
Jhamandas, K., Pinski, C., and Phillis, J. W. 1970. Effects of narcotic analgesics and antagonists on the in vivo release of acetylcholine from the cerebral cortex of the cat. Nature 228:176–177.
Sharkawi, M., and Schulman, M. P. 1969. Inhibition by morphine of the release of [14C] acetylcholine from rat cerebral cortical slices. J. Pharm. Pharmacol. 21:546–547.
Montel, H., Starke, K., and Weber, F. 1974. Influence of morphine and naloxone on the release of noradrenaline from rat brain cortical slices. Naunyn Schmiedebergs Arch. Pharmacol. 283: 357–369.
Werling, L. L., Brown, S. R., and Cox, B. M. 1987. Opioid receptor regulation of the release of norepinephrine in brain. Neuropharmacol. 26:987–996.
Loh, H. H., Brase, D. A., Sampath-Khanna, S., Mar, J. B., and Way, E. L. 1976. β-endorphine in vitro inhibition of striatal dopamine release. Nature 264:567–568.
Jessel, T. M., and Iversen, L. L. 1977. Oprate analgesics inhibit substance P release from rat trigeminal nucleus. Nature 268:549–551.
Iwatsubo, K., and Kondo, Y. 1978. The inhibitory effect of morphine on the release of preloaded3H-GABA from rat substantia nigra in response to stimulation of caudate nucleus and globus pallidus. p. 357in van Ree, J. M. and Terenius L. (eds.), Characteristics and Function of opioids, Elsevier/North Holland, Amsterdam.
Iversen, L. L., Inversen, S. D., and Bloom, F. E. 1980. Opiate receptors influence vasopressin release from nerve terminals in rat neurohypophysis. Nature 284:350–351.
Ipp, E., Dobbs, R., and Unger, R. H. 1978. Morphine and β-endorphin influence the secretion of the endocrine pancreas. Nature 276:190–191.
Kanter, R. A., Ensinck, J. W., and Fujimoto, W. Y. 1980. Disparate effects of enkephalin and morphine upon insulin and glucagon secretion by islets cell cultures. Diabetes 29:84–86.
Hescheler, R. L., Rosenthal, W., Trautwein, W., and Schultz, G. 1987. The GTP-binding protein G0 regulates neuronal calcium channels. Nature 325:445–447.
Henderson, G., and Seward, E. P. 1990. Inhibition of an N-like calcium channel by mu-opioid receptor activation in the human neuroblastoma cell line, SH-SY5Y. J. Physiol. 422:19P.
MacDonald, R. L., and Werz, M. A. 1986. Dynorphin A decreases voltage-dependent calcium conductance of mouse dorsal root ganglion neurons. J. Physiol. 377:237–249.
Attali, B., Saya, B., and Vogel, Z. 1989. Kappa opiate agonists inhibit adenylate cyclase and produce heterologous desensitization in rat spinal cord. J. Neurochem. 52:360–369.
Sharma, S. K., Klee, W. A., and Nirenberg, M. 1975. Dual regulation of adenylate cyclase accounts for narcotic dependence and tolerance. Porch. Natl. Acad. SCI. USA 72:3092–3096.
North, R. C. A., William's, J. E. T., Surpenant, A., and Christee, M. J. 1987. Mu and delta receptors belong to a family of receptors that are coupled to potassium channels. Porch. Nalt. Acad. SCI. USA 84:5487–5491.
Phillis, J. W., Mullin, W. J., and Pinski, C. 1973. Morphine enhancement of acetylcholine release into the lateral ventricle and from the cerebral cortex of unanaesthetized cats. Comp. Gen. Pharmacol. 4:189–200.
Mullin, W. J. 1974. Central release of acetylcholine following administration of morphine to unanesthetized rabbits. Canad. J. Physiol. Pharmacol. 52:369–374.
Fredholm, B. B., and Vernet, L. 1978. Morphine increases depolarization induced purine release from rat cortical slices. Acta Phys. Scand. 104:502–504.
Vizi, E. S., Harsing, L. G., and Knoll, J. 1977. Presynaptic inhibition leading to disinhibition of acetylcholine release from interneurons of the caudate nucleus: effect of dopamine, β-endorphin and d-ala2-pro5-enkephalinamide. Neurosci. 2:953–961.
Vizi, E. S., and Volbekas, V. 1980. Inhibition by dopamine of oxytocin release from isolated posterior lobe of the hypophysis of the rat: disinhibitory role of β-endorphine/enkephalin. Neuroendocrinol. 31:46–52.
Madison, D. V., and Nicoll, R. C. A. 1988. Enkephalin hyperpolarizes interneurones in the rat hippocampus. J. Physiol. 398: 123–130.
Pan, Z. Z., William's, J. E. T., and Osborne, P. B. 1990. Opioid actions on single nucleus raphe magnus neurons from rat and guinea pig in vitro. J. Physiol. 427:519–532.
Xu, H. Smolens, I., and Ginzler, A. R. 1989. Opioids can enhance and inhibit the electrically evoked release of methionineenkephalin. Brain Res. 504:36–42.
Xu, H., and Ginzler, A. R. 1992. Oploid enhancement of evoked [Met5] enkephalin release requires activation of cholinergic receptors: possible involvement of intracellular calcium. Proc. Natl. Acad. Sci. USA 89:1978–1982.
Hirai, K., and Katayama, Y. 1988. Methionine enkephalin presynaptically facilitates and inhibits bullfrog sympathetic ganglionic transmission. Brain Res. 448:299–307.
Mauborgne, A., Lutz, O., Legrand, J. C., Hamon, M., and Cesselin, F. 1987. Opposite effects of δ and μ opioid receptor agonists on the in vitro release of substance P-like material from rat spinal cord. J. Neurochem. 48:529–537.
Longoni, R., Spina, L., Mulas, A., Carboni, E., Garau, L., Melchiorri, P., and DiChiara, G. 1991. D-ala2, deltorphin II: D1-dependent stereotypes and stimulation of dopamine release in the nucleus accumbens. J. Neurosci. 11:1565–1576.
Suarez-Roca, H., and Maxiner, W. 1993. Activation of kappa opioid receptors by U50488H and morphine enhances the release of substance P from rat trigeminal nucleus slices. J. Pharmacol. Exp. Ther. 264:648–653.
Barke, K. E., and Hough, L. B. 1994. Characterization of basal and morphine induced histamine release in the rat periaqueductal gray. J. Neurochem. 63:238–244.
Cahill, C. M., White, T. D., and Sawynok, J. 1993. Morphine activates ω-conotoxin-sensitive Ca2+ channels to release adenosine from spinal cord synaptosomes. J. Neurochem. 60:894–901.
Kapas, S., Pubrick, A., and Hinson, J. P. 1995. Action of opioid peptides on the rat adrenal cortex: stimulation of steroid secretion through a specific μ opioid receptor. J. Endocrin. 144:503–510.
Michaelson, D. M., McDowall, G., and Sarne, Y. 1984. The Torpedo electric organ is a model for opiate regulation of acetylcholine release. Brain Res. 305:173–176.
Michaelson, D. M., McDowall, G., and Sarne, Y. 1984. Opiates inhibit acetylcholine release from Torpedo nerve terminals by blocking Ca2+ influx. J. Neurochem. 43:614–618.
Oron, L., Sarne, Y., and Michaelson, D. M. 1991. Effect of opioid peptides on electrically evoked acetylcholine release from Torpedo electromotor neurons. Neurosci. Lett. 125:231–234.
Barnea, E. R., Ashkenazy, R., and Sarne, Y. 1991. The effect of dynorphin on placental pulsatile human chorionic gonadotropin secretion in vitro. J. Clin. Endocrinol. Metab. 73:1093–1098.
Barnea, E. R., Askenazy, R., Tal, Y., Kol, S., and Sarne, Y. 1991. Effect of β-endorphin on human chorionic gonadotrophin secretion by placental explants. Human Reprod. 6:1327–1331.
Yu, V. C., Richards, M. L., and Sadee, W. 1986. A human neuroblastoma cell line expresses mu and delta opioid receptor sites. J. Biol. Chem., 261:1065–1070.
Baumhaker, Y., Gafni, M., Keren, O., and Sarne, Y. 1993. Selective and interactive down-regulation of mu and delta opioid receptors in human neuroblastoma SK-N-SH cells. Mol. Pharmacol. 44:461–467.
Noronha-Blob, L., Gover, R., and Baumgold, J. 1989. Calcium influx mediated by nicotinic receptors and voltage sensitive calcium channels in SK-N-SH human neuroblastoma cells. Biochem. Biophys. Res. Commun. 162:1230–1235.
Richards, M. C., and Sadee, W. 1986. Human neuroblastoma cell line as models of catechol uptake. Brain Res. 384:132–137.
Murphy, N. P., Ball, S. G., and Vaughan, F. T. 1991. Potassium-and carbachol-evoked release of [3H]noradrenaline from human neuroblastoma cells SH-SY5Y. J. Neurochem. 56:1810–1815.
Keren, O., Garty, M., and Sarne, Y. 1994. Dual regulation by opioids of3H-norepinephrine release in the human neuroblastoma cell line SK-N-SH. Brain Res. 646:319–323.
Jin, W., Lee, N. M., Loh, H. H., and Thayer, S. A. 1992. Dual excitatory and inhibitory effects of opioids on intracellular calcium in neuroblastoma × glioma hybrid NG108-15 cells. Mol. Pharmacol. 42:1083–1089.
Tomura, H., Okajima, F., and Kondo, Y. 1992. Enkephaline induces Ca2+ mobilization in single cells of bradykinin-sensitized differentiated neuroblastoma hybridoma (NG108-15) cells. Neurosci. Lett. 148:93–96.
Fields, A., Gafni, M., Oron, Y., and Sarne, Y. 1994. Multiple effects of opiates on intracellular calcium level and on calcium uptake in three neuronal cell lines. Brain Res. 687:94–102.
Tang, T., Kiang, J. G., and Cox, B. M. 1994. Opioids acting through delta receptors elicit a transient increase in the intracellular free calcium concentration in dorsal root ganglion—neu-roblastoma hybrid ND8-47 cells. J. Pharm. Exp. Ther. 270:40–46.
Connor, M. A., Planner, A., and Henderson, G. 1994. δ And μ opioid receptor mobilization of intracellular calcium in neuroblastoma cells. Regulatory Peptides 54:65–66.
Tai, K. K., Bian, C. F., and Wong, T. M. 1992. κ-Opioid receptor stimulation increases intracellular free calcium in isolated rat ventricular myocytes. Life SCI. 51:909–913.
Eriksson, P. S., Nilsson, M., Wagberg, M., Hansson, E., and Ronnback, L. 1993. Kappa-opioid receptors on astrocytes stimulate L-type Ca2+ channels. Neurosci. 54:401–407.
Stiene-Martin, A., Mattson, M. P., and Hauser, K. F. 1993. Opiates selectively increase intracellular calcium in developing type-1 astrocytes: role of calcium in morphine-induced morphologic differentiation. Dev. Brain Res. 76:189–196.
Higashi, H., Shinnick-Gallagher, P., and Gallagher, J. P. 1982. Morphine enhances and depresses Ca2+-dependent responses in visceral primary afferent neurons. Brain Res. 251:186–191.
Crain, S. M., Shen, K. F., and Chalazonitis, A. 1988. Opioids excite rather than inhibit sensory neurons after chronic opioid exposure of spinal cord ganglion cultures. Brain Res. 455:99–109.
Shen, K. F., and Crain, S. M. 1989. Dual opioid modulation of the action potential duration of mouse dorsal root ganglion neurons in culture. Brain Res. 491:227–242.
Barr, E., and Leslie, S. W. 1985. Opioid peptides increase calcium uptake by synaptosomes from brain regions. Brain Res. 329:280–284.
Laurent, S., Marsh, J. D., and Smith, T. W. 1986. Enkephalins increase cyclic adenosine monophosphate content, calcium uptake and contractile state in cultured chick embryo heart cells. J. Clin. Invest. 77:1436–1440.
Lorentz, M., Hedlund, B., and Arhem, P. 1988. Morphine activates calcium channels in cloned mouse neuroblastoma cell lines. Brain Res. 445:157–159.
Jin, W., Lee, N. M., Loh, H. H., and Thayer, S. A. 1994. Opioids mobilize calcium from inositol 1,45-triphosphate-sensitive stores in NG108-15 cells. J. Neurosci. 14:1920–1929.
Okajima, F., Tomura, H. and Kondo, Y. 1993. Enkephalin activates the phospholipase C/Ca2+ system through cross-talk between opioid receptors and P2-purinergic or bradykinin receptors in NG108-15 cells. Biochem. J. 290:241–247.
Ventura, C., Spurgeon, H., Lakatta, E. G., Guarnieri, C. and Capogrossi, M. C. 1992. κ And δ opioid receptor stimulation affects cardiac myocyte function and Ca2+ release from an intracellular pool in myocytes and neurons. Circul. Res. 70:66–81.
Natsuki, R., Hitzemann, R. J. and Loh, H. H. 1979. Influence of morphone, β-endorphin and naloxone on the synthesis of phosphoinositides in the rat midbrain. Res. Comm. Chem. Path. Pharm. 24:233–250.
Periyasamy, S. and Hoss, W. 1990. Kappa opioid receptors stimulate phosphoinositide turnover in rat brain. Life SCI. 47:219–225.
Barg, J., Belcheva, M. M., Rowinski, J. and Coscia, C. J. 1993. κ-Opioid agonist modulation of [3H]thymidine incorporation into DNA: Evidence for the involvement of pertussis toxin-sensitive G protein-coupled phosphoinositide turnover. J. Neurochem. 60: 1505–1511.
Barg, J., Nah, S. Y., Levy, R., Saya, D. and Vogel, Z. 1993. Modulation of thymidine incorporation by kappa-opioid ligands in rat spinal cord-dorsal root ganglion co-cultures. Brain Res. 629:109–114.
Smart, D., Smith, G., and Lambert, D. G. 1994. μ-Opioid receptor stimulation of inositol (1,4,5) triphosphate formation via a pertussis toxin-sensitive G protein. J. Neurochem. 62:1009–1014.
Smart, D., Smith, G., and Lambert, D. G. 1995. μ-Opioids activate phospholipase C in SH-SY5Y human neuroblastoma cells via calcium channel opening. Biochem. J. 305:577–582.
Smart, D., and Lambert, D. G. 1995. Desensitization of the μ-opioid activation of phospholipase C in SH-SY5Y cells: the role of protein kinases C and A and Ca2+-activated K+ currents. Br. J. Pharmacol. 116:2655–2660.
North, R. C. A., and William's, J. E. T. 1983. Opiate activation of potassium conductance inhibits calcium action potentials in rat locus coeruleus neurones. Br. J. Pharmacol. 80:225–228.
Werz, M. A., and McDonald, R. L. 1983. Opioid peptides with differential affinity for mu and delta receptors decrease sensory neuron calcium dependent action potentials. J. Pharmacol. Exp. Ther. 227:394–402.
Davis, J., and Duggan, A. W. 1974. Opiate agonist-antagonist effect on Renshaw cells and spinal interneurons. Nature 250:70–71.
Davis, J. 1976. Effects of morphine and naloxone on Renshaw cells and spinal interneurones in morphine dependent and non-dependent rats. Brain Res. 113:311–326.
Belcher, G., and Ryall, R. W. 1978. Differential excitatory and inhibitory effects of opiates on non-nociceptive and nociceptive neurones in the spinal cord of the cat. Brain Res. 145:303–314.
Fan, S. F., Shen, K. F., and Crain, S. M. 1993. μ And δ opioid agonists at low concentrations decrease voltage dependent K+ currents in F11 neuroblastoma x DRG neuron hybrid cells via cholera toxin sensitive receptors. Brain Res. 605:214–220.
Baraban, S. C., Lothman, E. W., Lee, A., and Guyenet, P. G. 1995. Kappa opioid receptor-mediated suppression of voltage activated potassium current in a catecholaminergic neuronal cell line. J. Pharmacol. Exp. Ther. 273:927–933.
Fan, S. F., and Crain, S. M. 1995. Dual regulation by mu, delta and kappa opioid receptor agonists of K+ conductance of DRG neurons and neuroblastoma x DRG neuron hybrid F11 cells. Brain Res. 696:97–105.
Fan, S. F., Shen, K. F., and Crain, S. M. 1991. Opioids at low concentration decrease opening of K+ channels in sensory ganglion neurons. Brain Res. 558:166–170.
Traber, J., Gullis, R., and Hamprecht, B. 1975. Influence of opiates on the levels of adenosine 3′∶5′ cyclic monophosphate in neuroblastoma x glioma hybrid cells. Life Sci. 16:1863–1868.
Collier, H. O. J., Francis, D. L., McDonald-Gibson, W. J., Roy, A. C., and Saeed, S. A. 1975. Prostaglandins, cyclic AMP and the mechanism of opiate dependence. Life Sci. 17:85–90.
Puri, S. K., Cochin, J., and Volicer, L. 1975. Effect of morphine sulfate on adenylate cyclase and phosphodiesterase activities in rat corpus striatum. Life Sci. 16:759–768.
Lee, A. Y. S., and Wang, T. M. 1987. Effects of dynorphin1–13 on cardiac rhythm and cyclic adenosine monophosphate (cAMP) levels in the isolated perfused rat heart. Neurosci. Lett. 80:289–292.
Makman, M. H., Dvorkin, B., and Crain, S. M. 1988. Modulation of adenylate cyclase activity of mouse spinal cord-ganglion explants by opioids, serotonin and pertussis toxin. Brain Res. 445: 303–313.
Onali, P., and Olianas, M. C. 1991. Naturally occurring opioid receptor agonists stimulate adenylate cyclase activity in rat olfactory bulb. Mol. Pharmacol. 39:436–441.
Olianas, M. C., and Onali, P. 1994. Activation of opioid and muscarinic receptors stimulates basal adenylate cyclase but inhibits Ca2+/calmodulin- and forskolin-stimulated enzyme activities in rat olfactory bulb. J. Neurochem. 63:161–168.
Cruciani, R. A., Dvorkin, B., Morris, S. A., Crain, S. M., and Makman, M. H. 1993. Direct coupling of opioid receptors to both stimulatory and inhibitory guanine nucleotide binding proteins in F11 neuroblastoma sensory neuron hybrid cells. Proc. Natl. Acad. Sci. USA 90:3019–3023.
Wang, L., and Gintzler, A. R. 1994. Bimodal opioid regulation of cyclic AMP formation: implications for positive and negative coupling of opiate receptors to adenylate cyclase. J. Neurochem. 63:1726–1730.
Wang, L., and Gintzler, A. R. 1995. Morphine tolerance and physical dependence: reversal of opioid inhibition to enhancement of cyclic AMP formation. J. Neurochem. 64:1102–1106.
Mehta, C. S., and Strada, S. J. 1994. Effects of acute and continuous administration of morphine on the cyclic AMP response induced by norepinephrine in rat brain slices. Life Sci. 55:35–42.
Periyasamy, S., and Hoss, W. 1991. Inhibition of carbachol stimulated phosphoinositide turnover by U-50,488H in rat hippocampus-involvement of GTP-binding protein. Eur. J. Pharmacol. 207: 101–109.
Beani, L., Bianchi, C., and Siniscalchi, A. 1982. The effect of naloxone on opioid-induced inhibition and facilitation of acetylcholine release in brain slices. Br. J. Pharmacol. 76:393–401.
Suarez-Roca, H., and Maixner, W. 1995. Morphine produces a biphasic modulation of substance P release from cultured dorsal root ganglion neurons. Neurosci. Lett. 194:41–44.
Benoliel, J. J., Collin, E., Mauborgne, A., Bourgoin, S., Legrand, J. C., Hamon, M., and Cesselin, F. 1994. Mu and delta opioid receptors mediate opposite modulations by morphine of the spinal release of cholecystokinin-like material. Brain Res. 653:81–91.
Crain, S. M., and Shen, K. F. 1992. After chronic opioid exposure sensory neurons become supersensitive to the excitatory effects of opioid agonists and antagonists as occurs after acute elevation of GM1 ganglioside. Brain Res. 575:13–24.
Sarne, Y., and Gafni, M. 1996. Determinants of the stimulatory opioid effect on intracellular calcium in SK-N-SH and NG108-15 neuroblastoma. Brain Res. 722:203–206.
McKenzie, F. R., and Milligan, G. 1990. δ-Opioid-receptor-mediated inhibition of adenylate cyclase is transduced specifically by the guanine-nucleotide binding protein Gi2. Biochem. J. 267: 391–398.
Shen, K. F., and Crain, S. M. 1990. Cholera toxin-A subunit blocks opioid excitatory effects on sensory neuron action potentials indicating mediation by Gs-linked opioid receptors. Brain Res. 525:225–231.
Gintzler, A. R., and Xu, H. 1991. Different G proteins mediate the opioid inhibition or enhancement of evoked [5-methionine] enkephalin release. Proc. Natl. Acad. Sci. USA 88:474–475.
Tang, T., Kiang, J. G., Cote, T., and Cox, B. M. 1995. Opioid-induced increase in [Ca2+]i in ND8-47 neuroblastoma x dorsal root ganglion hybrid cells is mediated through G protein-coupled δ-opioid receptors and desensitized by chronic exposure to opioid. J. Neurochem. 65:1612–1621.
Tang, T., Kiang, J. G., Cote, T. E., and Cox, B. M. 1995. Antisense oligodeoxynucleotide to the Gi2 protein α subunit sequence inhibits an opioid-induced increase in the intracellular free calcium concentration in ND8-47 neuroblastoma x dorsal root ganglion hybrid cells. Mol. Pharmacol. 48:189–193.
Tang, W. J., and Gilman, A. G. 1991. Type-specific regulation of adenylyl cyclase by G protein βγ subunits. Science 254:1500–1503.
Tang, W. J., and Gilman, A. G. 1992. Adenylyl cyclases. Cell 70:869–872.
Camps, M., Carozzi, A., Schnabel, P., Scheer, A., Parker, P. J., and Gierschik, P. 1992. Isoenzyme-selective stimulation of phospholipase C-β2 by G protein βγ subunits. Nature 360:684–689.
Olianas, M. C., and Onali, P. 1993. Synergistic interaction of muscarinic and opioid receptors with Gs-linked neurotransmitter receptors to stimulate adenylyl cyclase activity of rat olfactory bulb. J. Neurochem. 61:2183–2190.
Kaneko, S., Nakamura, S., Adachi, K., Akaike, A., and Satoh, M. 1994. Mobilization of intracellular Ca2+ and stimulation of cyclic AMP production by κ opioid receptors expressed in Xenopus oocytes. Mol. Brain Res. 27:258–264.
Tsu, R. C., Chan, J. S., and Wong, Y. H. 1995. Regulation of multiple effectors by the cloned δ-opioid receptor: stimulation of phospholipase C and type II adenylyl cyclase. J. Neurochem. 64: 2700–2707.
Birnbaum, A. K., Wotta, D. R., Law, P. Y., and Wilcox, G. L. 1995. Functional expression of adrenergic and opioid receptors in Xenopus oocytes: interaction between α2- and α2-adrenergic receptors. Mol. Brain Res. 28:72–80.
Zimprich, A., Simon, T., and Holt, V. 1995. Transfected rat μ opioid receptors (rMOR1 and rMOR1B) stimulate phospholipase C and Ca2+ mobilization. Neuroreport 7:54–56.
Miyamae, T., Fukushima, N., Misu, Y., and Ueda, H. 1993. δ Opioid receptor mediates phospholipaseC activation via Gi in Xenopus oocytes. FEBS Lett. 333:311–314.
Ueda, H., Miyamae, T., Fukushima, N., Takeshima, H., Fukuda, K., Sasaki, Y., and Misu, Y. 1995. Opioid mu- and kappa-receptors mediate phospholipase C activation through Gi1 in Xenopus oocytes. Mol. Brain Res. 32:166–170.
Chan, J. S. C., Chiu, T. T., and Wong, Y. H. 1995. Activation of type II adenylyl cyclase by the cloned mu-opioid receptor: coupling to multiple G proteins. J. Neurochem. 65:2682–2689.
Chen, G. G., Chalazonitis, A., Shen, K. F., and Crain, S. M. 1988. Inhibitor of cyclic AMP-dependent protein kinase blocks opioid-induced prolongation of the action potential of mouse sensory ganglion neurons in dissociated cell cultures. Brain Res. 462:372–377.
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Sarne, Y., Fields, A., Keren, O. et al. Stimulatory effects of opioids on transmitter release and possible cellular mechanisms: Overview and original results. Neurochem Res 21, 1353–1361 (1996). https://doi.org/10.1007/BF02532376
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DOI: https://doi.org/10.1007/BF02532376