Abstract
Growth ofHelicobacter pylori in liquid culture requires the addition of media supplements that often interfere with subsequent purification of bacterial antigens. In order to determine whether cyclodextrins can substitute for conventionalH. pylori growth supplements, we culturedH. pylori in the presence of five commercially available cyclodextrins. The effect of these compounds on the production of the vacuolating cytotoxin antigen was evaluated. Several cyclodextrins supported flourishing growth and permitted the consistent production of vacuolating cytotoxin. These data suggest that Brucella broth supplemented with cyclodextrins is an improved medium for bacterial culture and industrial production ofH. pylori antigens.
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Abbreviations
- αCD :
-
α Cyclodextrin
- βCD β:
-
Cyclodextrin
- γCD γ:
-
Cyclodextrin
- dMeβCD :
-
2,6-Di-O-methyl)-β-cyclodextrin
- tMeβCD :
-
2,3,6-Tri-O-methyl)-β-cyclodextrin
- CDs :
-
Cyclodextrins
- VacA :
-
Vacuolating cytotoxin
- BB :
-
Brucella broth
- CBA :
-
Columbia broth agar
- FCS :
-
Feral calf serum
References
Blaser MJ (1990)Helicobacter pylori and the pathogenesis of gastroduodenal inflammation, J Infect Dis 161:629–633
Buck GE, Smith JS (1987) Medium supplementation for growth ofCampylobacter pyloridis, J Clin Microbiol 29:597–599
Cover TL, Blaser MJ (1992)Helicobacter pylori and gastroduodenal disease. Annu Rev Med 43:135–136
Forman D, Newell DG, Fullerton F, Yarnell JW, Stacey AR, Wald N, Sitas F (1991) Association between infection withHelicobacter pylori and risk of gastric cancer: evidence from a prospective investigation. Br Med J 302:1302–1305
Hazell SL, Graham DY (1990) Unsaturated fatty acids and viability ofHelicobacter (Campylobacter) pylori. J Clin Microbiol 28:1060–1061
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
Morgan DR, Freedman R, Depew CE, Kraft WG (1987) Growth ofCampylobacter pylori in liquid media. J Clin Microbiol 25: 2123–2125
Olivieri R, Bugnoli M, Armellin D, Bianciardi S, Rappuoli R, Bayeli PF, Abate L, Esposito E, De Gregorio L, Aziz J, Casagni C, Figura N (1993) Growth ofHelicobacter pylori in media containing cyclodextrins. J Clin Microbiol 31:160–162
Parsonnet J, Friedman GD, Vandersteen DP, Chang Y, Vogelman JH, Orentreich N, Sibley RK (1991)Helicobacter pylori infection and the risk of gastric carcinoma. N Engl J Med 325: 1127–1131
Shahamat M, Mai UEH, Paszko-Kolva C, Yamamoto H, Colwell RR (1991) Evaluation of liquid media for growth ofHelicobacter pylori. J Clin Microbiol 29:2835–283
Telford JL, Ghiara P, Dell’Orco M, Comanducci M, Burroni D, Bugnoli M, Tecce MF, Censini S, Covacci A, Xiang Z, Papini E, Montecucco C, Parente L, Rappuoli R (1994) Gene structure of theHelicobacter pylori cytotoxin and evidence of its key role in gastric disease. J Exp Med 179:1653–1658
Thompson L, Cockayne A, Spiller RC (1994) Inhibitory effect of polyunsaturated fatty acids on the growth ofHelicobacter pylori: a possible explanation of the effect of diet on peptic ulceration. Gut 35:1557–1561
Warren JR, Marshall BJ (1983) Unidentified curved bacilli on gastric epithelium in active chronic gastritis. Lancet II:1273–1275
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Marchini, A., d’Apolito, M., Massari, P. et al. Cyclodextrins for growth ofHelicobacter pylori and production of vacuolating cytotoxin. Arch. Microbiol. 164, 290–293 (1995). https://doi.org/10.1007/BF02529963
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DOI: https://doi.org/10.1007/BF02529963