Skip to main content
SpringerLink
Log in

Expression of the mutantArabidopsis thaliana acetolactate synthase gene confers chlorsulfuron resistance to transgenic poplar plants

  • Papers
  • Published:
Transgenic Research Aims and scope Submit manuscript

Abstract

The mutant acetolactate synthase (crs1-1) gene fromArabidopsis thaliana, which confers resistance to the herbicide chlorsulfuron, was transferred to a hybrid poplar (Populus tremula×P. alba) using twoAgrobacterium-mediated transformation methods (co-inoculation and co-cultivation). Two different constructs were used. In one, the mutantcrs1-1 gene was placed under the control of its own promoter, and, in the other, this gene was under the control of the duplicated cauliflower mosaic virus 35S promoter (70 promoter). The transformation efficiency ranged from 22 to 32% of the tumours in co-inoculation and from 67 to 77% of the stem explants in co-cultivation experiments. The usefulness of the herbicide chlorsulfuron as a selectable marker gene was also demonstrated. Successful genetic transformation was verified by Southern and northern analyses and enzyme activity. Plants carrying thecrs1-1 mutant gene under the control of the 70 promoter showed high levels of transcription and activity whereas plants carrying the nativecrs1-1 gene showed low levels of expression. However, transgenic plants expressing each of the chimaericcrs1-1 genes are completely resistant to high doses of chlorsulfuron in greenhouse tests.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Atkinson, R.G. and Gardner, R. (1991)Agrobacterium-mediated transformation of pepino and regeneration of transgenic plants.Pl. Cell Rep. 10, 208–12.

    Article  CAS  Google Scholar 

  • Brasileiro, A.C.M., Leplé, J.C., Muzzin, J., Ounnoughi, D., Michel, M.F. and Jouanin, L. (1991) An alternative approach for gene transfer in trees using wild-typeAgrobacterium strains.Pl. Mol. Biol. 17, 441–52.

    Article  CAS  Google Scholar 

  • Chaleff, R.S. and Mauvais, C.J. (1984) Acetolactate synthase is the site of two sulfonylurea herbicides in higher plants.Science 224, 1443–5.

    Article  CAS  PubMed  Google Scholar 

  • Charest, P.J., Hattori, J., DeMoor, J., Iyer, V.N. and Miki, B.L. (1990)In vitro study of transgenic tobacco expressingArabidopsis wild type and mutant acetohydroxyacid synthase genes.Pl. Cell Rep. 8, 643–6.

    Article  CAS  Google Scholar 

  • De Block, M. (1990) Factors influencing the tissue culture and theAgrobacterium tumefaciens-mediated transformation of hybrid aspen and poplar clones.Pl. Physiol. 93, 1110–6.

    Google Scholar 

  • Dellaporta, S.L., Wood, J. and Hicks, J.B. (1983) A plant DNA minipreparation: version II.Pl. Mol. Biol. Rep. 1, 19–21.

    Article  CAS  Google Scholar 

  • Fillatti, J.J., Sellmer, J.C., McCown, B.H., Haissig, B.E. and Comai, L. (1987)Agrobacterium-mediated transformation and regeneration ofPopulus.Mol. Gen. Genet. 206, 192–9.

    Article  CAS  Google Scholar 

  • Fillatti, J.A., Haissig, B., McCown, B., Comai, L. and Reimenschneider, D. (1988) Development of glyphosate-tolerantPopulus plants through expression of a mutantaroA gene fromSalmonella typhimurium. In Hanover, J.W. and Keathley, D.E. (eds),Genetic manipulation of woody plants, pp. 243–9. New York and London: Plenum Press.

    Google Scholar 

  • Gabard, J.M., Charest, P.J., Iyer, V.N. and Miki, B.L. (1989) Cross-resistance to short residual sulfonylurea herbicides in transgenic tobacco plants.Pl. Physiol. 91, 574–80.

    CAS  Google Scholar 

  • Haughn, G.W. and Somerville, C. (1986) Sulfonylurea-resistant mutants ofArabidopsis thaliana.Mol. Gen. Genet. 204, 430–4.

    Article  CAS  Google Scholar 

  • Haughn, G.W., Smith, J., Mazur, B. and Sommerville, C. (1988) Transformation with a mutantArabidopsis acetolactate syntase gene renders tobacco resistant to sulfonylurea herbicides.Mol. Gen. Genet. 211, 266–71.

    Article  CAS  Google Scholar 

  • Jones, J.D.G., Dunsmuir, P. and Bedbrook, J. (1985) High level expression of introduced chimaeric genes in regenerated transformed plants.EMBO J. 4, 2411–8.

    CAS  PubMed  Google Scholar 

  • Kay, R., Chan, A., Daly, M. and McPherson, J. (1987) Duplication of CaMV35S promoter sequences creates a strong enhancer for plant genes.Science 236, 1299–302.

    Article  CAS  PubMed  Google Scholar 

  • Koncz, C. and Schell, J. (1986) The promoter of TL-DNA gene 5 controls the tissue-specific expression of chimaeric genes carried by a novel type ofAgrobacterium binary vector.Mol. Gen. Genet. 204, 383–96.

    Article  CAS  Google Scholar 

  • Leplé, J.C., Brasileiro, A.C.M., Michel, M.F., Delmotte, F. and Jouanin, L. (1991) Transgenic poplars: expression of chimeric genes in four different constructs.Pl. Cell Rep. (in press).

  • Mazur, B.J., Chui, C.F. and Smith, J.K. (1987a) Isolation and characterization of plant genes coding for acetolactate synthase, the target enzyme for two classes of herbicides.Pl. Physiol. 85, 1110–17.

    CAS  Google Scholar 

  • Mazur, B.J., Falco, S.C., Knowlton, S. and Smith, J.K. (1987b) Acetolactate synthase, the target enzyme of the sulfonylurea herbicides. In: von Wettstein, D. and Chua, N.H., eds,Plant Molecular Biology. New York: Plenum Press.

    Google Scholar 

  • Mazur, B.J. and Falco, S.C. (1989) The development of herbicide resistant crops.Ann. Rev. Plant Physiol. 40, 441–70.

    Article  CAS  Google Scholar 

  • McCown, B.H., McCabe, D.E., Russell, D.R., Robison, D.J., Barton, K.A. and Raffa, K.F. (1991) Stable transformation ofPopulus and incorporation of pest resistance by electric discharge particle acceleration.Pl. Cell Rep. 9, 590–4.

    Article  CAS  Google Scholar 

  • McGranahan, G.H., Leslie, C.A., Uratsu, S.L., Martin, L.A. and Dandekar, A.M. (1988)Agrobacterium-mediated transformation of walnut somatic embryos and regeneration of transgenic plants.Bio/Technology 6, 800–4.

    Article  CAS  Google Scholar 

  • McHughen, A. (1989)Agrobacterium-mediated transfer of chlorsulfuron resistance to commercial flax cultivars.Pl. Cell Rep. 8, 445–9.

    Article  CAS  Google Scholar 

  • Michel, M.F., Brasileiro, A.C.M., Depierreux, C., Otten, L., Delmotte, F. and Jouanin, L. (1990) Identification of differentAgrobacterium strains isolated from the same forest nursery.Appl. Environ. Microbiol. 56, 3537–45.

    PubMed  Google Scholar 

  • Miki, B.L., Labbé, H., Hattori, J., Ouellet, T., Gabard, J., Sunohara, G., Charest, P.J. and Iyer, V.N. (1990) Transformation ofBrassica napus canola cultivars withArabidopsis thaliana acetohydroxyacid synthase genes and analysis of herbicide resistance.Theor. Appl. Genet. 80, 449–58.

    Article  CAS  Google Scholar 

  • Murashige, T. and Skoog, F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures.Physiol. Pl. 15, 473–97.

    Article  CAS  Google Scholar 

  • Naina, N.S., Gupta, P.K. and Mascarenhas, A.F. (1989) Genetic transformation and regeneration of transgenic neem (Azadirachta indica) plants usingAgrobacterium tumefaciens.Curr. Sci. 58, 184–7.

    Google Scholar 

  • Odell, J.T., Caimi, P.G., Yadav, N.S. and Mauvais, C.J. (1990) Comparation of increased expression of wild-type and herbicide-resistant acetolactate synthase genes in transgenic plants, and indication of post transcriptional limitation on enzyme activity.Pl. Physiol. 94, 1647–54.

    Article  CAS  Google Scholar 

  • Olsen, W.L. (1988) Molecular biology in forestry research: a review. In Valentine, A. ed.,Forest and Crop Biotechnology: Progress and Prospects, pp. 315–34. New York: Springer-Verlag.

    Google Scholar 

  • Oxtoby, E. and Hughes, M.A. (1990) Engineering herbicide tolerance into crops.TIBS 8, 61–5.

    CAS  Google Scholar 

  • Parsons, T.J., Sinkar, V.P., Stettler, R.F., Nester, E.W. and Gordon, M.P. (1986) Transformation of poplar byAgrobacterium tumefaciens.Bio/Technology 4, 533–6.

    Article  CAS  Google Scholar 

  • Phelep, M., Petit, A., Martin, L., Duhoux, E. and Tempé, J. (1991) Transformation and regeneration of a nitrogen-fixing tree,Allocasuarina verticillata Lam.Bio/Technology 9, 461–6.

    Article  CAS  Google Scholar 

  • Pimentel, D., Hunter, M.S., LaGro, J.A. Efroymson, R.A., Landers, J.C., Mervis, F.T., McCarthy, C.A. and Boyd, A.E. (1989) Benefits and risks of genetic engineering in agriculture.BioScience 39, 606–14.

    Article  Google Scholar 

  • Ray, T.B. (1986) Sulfonylurea herbicides as inhibitors of amino acid biosynthesis in plants.TIBS 11, 180–3.

    CAS  Google Scholar 

  • Riemenschneider, D.E. (1990) Susceptibility of intra- and interspecific hybrid poplars toAgrobacterium tumefaciens strains C58.Phytopathology 80, 1099–102.

    Google Scholar 

  • Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989)Molecular Cloning: a Laboratory Manual. New York: Cold Spring Harbor Laboratory Press.

    Google Scholar 

  • Sanders, P.R., Winter, J.A., Barnason, A.R., Rogers, S.G. and Fraley, R.T. (1987) Comparation of cauliflower mosaic virus 35S and nopaline synthase promoters in transgenic plants.Nucl. Acids Res. 15, 1543–58.

    CAS  PubMed  Google Scholar 

  • Sellmer, J.C. and McCown, B.H. (1989) Transformation inPopulus spp. In: Bajaj, Y.P.S. ed.,Plant Protoplasts and Genetic Engineering II, pp. 155–72. Heidelberg, Berlin: Springer-Verlag.

    Google Scholar 

  • Tepfer, M. and Casse-Delbart, F. (1987)Agrobacterium rhizogenes as a vector for transforming higher plants.Microbiol Sci. 4, 24–8.

    CAS  PubMed  Google Scholar 

  • Turgeon, R. (1982) Teratomas and secondary tumors. In Kahl, G. and Schell, J.S. eds,Molecular Biology of Plant Tumors, pp. 391–414. New York: Academic Press.

    Google Scholar 

  • Verwoerd, T.C., Dekker, B.M.M. and Hoekema, A. (1989) A small-scale procedure for rapid isolation of plant RNAs.Nucl. Acids Res. 17, 2362.

    CAS  PubMed  Google Scholar 

  • Vilaine, F. and Casse-Delbart, F. (1987) A new vector derived fromAgrobacterium rhizogenes plasmids: a micro-Ri plasmid and its use to construct a mini-Ri plasmid.Gene 55, 105–14.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratoire de Biologie Cellulaire, INRA, route de Saint-Cyr, F-78026, Versailles Cedex, France

    Ana Cristina Miranda Brasileiro, Colette Tourneur & Lise Jouanin

  2. Station d’Amélioration des Arbres Forestiers, INRA, Ardon, F-45160, Olivet, France

    Jean-Charles Leple & Valérie Combes

Authors
  1. Ana Cristina Miranda Brasileiro
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Colette Tourneur
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jean-Charles Leple
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Valérie Combes
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Lise Jouanin
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Brasileiro, A.C.M., Tourneur, C., Leple, JC. et al. Expression of the mutantArabidopsis thaliana acetolactate synthase gene confers chlorsulfuron resistance to transgenic poplar plants. Transgenic Research 1, 133–141 (1992). https://doi.org/10.1007/BF02528778

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02528778

Keywords

Search

Navigation