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Stable transformation of Populus and incorporation of pest resistance by electric discharge particle acceleration

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Abstract

Three different target tissues (protoplast-derived cells, nodules, and stems) and two unrelated hybrid genotypes of Populus (P. alba x P. grandidentata ‘Crandon’ and P. nigra ‘Betulifolia’ x P. trichocarpa) have been stably transformed by electric discharge particle acceleration using a 18.7 kb plasmid containing NOS-NPT, CaMV 35S-GUS, and CaMV 35S-BT. Four transformed plants of one hybrid genotype, NC5339, containing all 3 genes were recovered and analyzed. Two expressed GUS and one was highly resistant to feeding by 2 lepidopteran pests (the forest tent caterpillar, Malacosoma disstria, and the gypsy moth, Lymantria dispar.) Pretreatment of the target tissues, fine-tuning of the bombardment parameters, and the use of a selection technique employing flooding of the target tissues were important for reliable recovery of transformed plants.

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Abbreviations

BA:

benzyladenine

NAA:

1-naphthaleneacetic acid

TDZ:

thidiazuron

DB:

2,6-dichlorobenzonitrile

WPM:

woody plant medium

Kan:

kanamycin

Carb:

carbenicillin

Ben:

benylate

gus:

β-glucuronidase (EC 3.2.1.31)

NPT:

neomycin phosphoransferase (EC 2.7.1.95) gene

GUS:

betaglucuronidase gene

BT:

modified endotoxin gene originally from Bacillus thuringiensis (B.t.)

PCR:

polymerase chain reaction

References

  • Barton KA, Whiteley HR, Yang N (1987) Plant Physiol 85:1103–1109.

    Google Scholar 

  • Bekkaoui F, Pilon M, Laine E, Raju DSS, Crosby WL, Dunstan DI (1988) Plant Cell Rep 7:481–484.

    Google Scholar 

  • Beremand PD, Hannapel DJ, Guerra DJ, Kuhn DN, Ohlrogge JB (1987) Arch Biochem Biophys 256:90–100.

    Google Scholar 

  • Christou P, Swain W, Yang N.-S, McCabe D (1989) PNAS 86:7500–7504.

    Google Scholar 

  • Dellaporta S, Wood J, Hicks J (1983) Plant Mol Biol Rep 1:19–21.

    CAS  Google Scholar 

  • Fillatti JJ, Sellmer J, McCown B, Haissig B, Comai L. (1987) Mol Gen Genet 206:192–199.

    Google Scholar 

  • Fischhoff DA, Bowdisk KS, Perlak FJ, Marrone PG, McCormick SM, Niedermeyer JG, Dean DA, Kusano-Kretzner K, Meyer FJ, Rochester DE, Rogers SG, Fraley RT (1987) Bio/Technology 5:807–813.

    Google Scholar 

  • Galbraith DW, Shields BA (1982) Physiol Plant 55:25–30.

    Google Scholar 

  • Grisdale D (1985) In: Singh P, Moore RS (eds), Handbook of Insect Rearing, Vol. II, Elsevier Publishing, NY, pp. 369–379.

    Google Scholar 

  • Heimpel AM, Angus TA (1959) J Ins Path 1:152–170.

    Google Scholar 

  • James DJ, Passey, AJ, Barbara DJ, Bevan M (1989) Plant Cell Rep 7:658–661.

    Google Scholar 

  • Jefferson RA, Kavanagh TA, Bewan MW (1987) EMBO J 6:3901–3907.

    CAS  PubMed  Google Scholar 

  • Krieg A, Langenbruch GA (1981) In: H.D. Burgess HD (ed.), Microbial Control of Pests and Plant Diseases, 1970–1980, Academic Press, N.Y. pp. 837–1097.

    Google Scholar 

  • Lloyd G, McCown B (1980) Proc Int Plant Prop Soc 30:420–427.

    Google Scholar 

  • McCabe DE, Swain WF, Martinell BJ, Christou P. (1988) Bio/Technology 6:923–926.

    Google Scholar 

  • McCown BH, Zeldin E, Pinkalla H, Dedolph R (1988) In: Hanover J, Keathley D (eds), Genetic Manipulation of Woody Plants, Plenum Press, Seattle, WA, pp. 149–166.

    Google Scholar 

  • McGranahan G, Leslie C, Uratsu S, Martin L, Dandekar A (1988) Bio/Technology 6:800–804.

    Google Scholar 

  • Montgomery ME, Wallner WE (1988) In: Berryman AA (ed.), Dynamics of Forest Insect Populations, Plenum Press, N.Y., pp. 354–375.

    Google Scholar 

  • Murray M, Thompson W (1980) Nucl Acids Res 8:4321–4325.

    Google Scholar 

  • Pythoud F, Sinkar VP, Nester EW, Gordon MP (1987) Bio/Technology 5:1323–1327.

    Google Scholar 

  • Raffa KF (1989) BioScience 39:524–534.

    Google Scholar 

  • Ranney JW, Wright LL, Layton PA (1987) J For 85:17–28.

    Google Scholar 

  • Russell JA, McCown BH (1988) Plant Cell Rep 7:59–62.

    Google Scholar 

  • Saiki R, Gelfaud D, Stoffel S, Scharf S, Higuchi R, Horn G, Mullis K, Erlich, H (1988) Science 239:487–494.

    CAS  PubMed  Google Scholar 

  • SAS (1982) SAS Users Guide: Statistics. SAS Institute, Cary, NC.

    Google Scholar 

  • Schnepf HE, Wong HC, Whiteley HR (1985) J Biol Chem 260:6264–6272.

    Google Scholar 

  • Seguin A, Lalonde M (1988) Plant Cell Rep 7:367–370.

    Google Scholar 

  • Sellmer JC, McCown BH (1989) In: Bajaj YPS (ed) Plant Protoplasts and Genetic Engineering II, Springer Verlag, Berlin. pp. 155–172.

    Google Scholar 

  • Sellmer JC, McCown BH, Haissig BE (1989) Tree Physiol 5:219–227.

    Google Scholar 

  • Vaeck M, Reynaerts A, Hofte H, Jensens S, DeBeuckeleer M, Dean C, Abeau M, VanMontagu M, Leemand J (1987) Nature 328:33–37.

    Google Scholar 

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Authors and Affiliations

  1. Department of Horticulture, University of Wisconsin-Madison, 53706, Madison, WI, USA

    B. H. McCown

  2. Department of Entomology, University of Wisconsin-Madison, 53706, Madison, WI, USA

    D. J. Robison & K. F. Raffa

  3. Agracetus, 53562, Middleton, WI, USA

    D. E. McCabe, D. R. Russell & K. A. Barton

Authors
  1. B. H. McCown
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  2. D. E. McCabe
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  3. D. R. Russell
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  4. D. J. Robison
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  5. K. A. Barton
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  6. K. F. Raffa
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Additional information

Communicated by J.M. Widholm

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McCown, B.H., McCabe, D.E., Russell, D.R. et al. Stable transformation of Populus and incorporation of pest resistance by electric discharge particle acceleration. Plant Cell Reports 9, 590–594 (1991). https://doi.org/10.1007/BF00232339

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  • DOI: https://doi.org/10.1007/BF00232339

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