Summary
Plant myosins are motor proteins that bind to the external surfaces of organelles and interact with the cytoskeletal protein actin (as actin microfilaments), which organizes and directs intracellular movement. Recent progress in physiological, biochemical, immunological, and genetical studies of plant myosin has revealed considerable information about the structures and functions of these important molecules. This article briefly reviews the history of plant myosin research, summarizes recent progress, and highlights directions for future research.
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Abbreviations
- AF:
-
actin filament
References
Barry WH (1968) Coupling of excitation and cessation of cyclosis in Nitella: role of divalent cations. J Cell Physiol 72: 153–160
Boevink P, Oparka K, Santa-Cruz S, Martin B, Betteridge A, Hawes C (1998) Stacks on track: the plant Golgi apparatus traffic on an actin/ER network. Plant J 15: 441–447
Braun M (1996) Immunolocalization of myosin in rhizoids ofChara globularis Thuill. Protoplasma 191: 1–8
Chen JCW, Kamiya N (1975) Localization of myosin in the internodal cell ofNitella as suggested by differential treatment with N-ethylmaleimide. Cell Struct Fund 1: 1–9
Cheney RE, Riley MA, Mooseker MS (1993a) Phylogenetic analysis of the myosin superfamily. Cell Motil Cytoskeleton 24: 215–223
Cheney RE, O’Shea MK, Heuser JE, Coelho MV, Wolenski JS, Espreafico EM, Forscher P, Larson RE, Mooseker MS (1993b) Brain myosin V is a two-headed unconventional myosin with motor activity. Cell 75: 13–23
Cope MJTV, Whisstock J, Rayment I, Kendrick-Jones J (1996) Conservation within the myosin motor domain: implications for structure and function. Structure 4: 969–987
Forde J, Steer MW (1976) Cytoplasmic streaming inElodea. Can J Bot 54: 2688–2694
Goosen-de Roo L, Burggraff PD, Libbenga KR (1983) Microfilament bundles associated with tubular endoplasmic reticulum in fusiform cells in the active cambial zone ofFraxinus excelsior L. Protoplasma 116: 204–208
Grolig F, Williamson RE, Parke J, Miller C, Anderton BH (1988) Myosin and Ca2+-sensitive streaming in the alga Chara: detection of two polypeptides reacting with a monoclonal anti-myosin and their localization in the streaming endoplasm. Eur J Cell Biol 47: 22–31
—, Schroder J, Sawitzky H, Lange U (1996) Partial characterization of a putative 110 kDa myosin from the green algaChara corallina by in vitro binding of fluorescent F-actin. Cell Biol Int 20: 365–373
Hayama T, Tazawa M (1980) Ca2+ reversibly inhibits active rotation of chloroplasts in isolated cytoplasmic droplets ofChara. Protoplasma 102: 1–9
Hensel W (1985) Cytochalasin B affects the structural polarity of statocytes from cress roots (Lepidium sativum). Protoplasma 129: 178–187
Heslop-Harrison J, Heslop-Harrison Y (1989) Myosin associated with the surface of organelles, vegetative nuclei and generative cells in angiosperm pollen grains and tubes. J Cell Sci 94: 319–325
Higashi-Fujime S, Ishikawa R, Iwasawa H, Kagami O, Kurimoto E, Kohama K, Hozumi T (1995) The fastest actin-based motor protein from the green alga,Chara, and its distinct mode of interaction with actin. FEBS Lett 375: 151–154
Igarashi H, Vidali L, Yokota E, Sonobe S, Hepler PK, Shimmen T (1999) Actin filaments purified from tobacco cultured BY-2 cells can be translocated by plant myosin. Plant Cell Physiol 40: 1167–1171
Kachar B, Reese TS (1988) The mechanism of cytoplasmic streaming in characean algal cells: sliding of endoplasmic reticulum along actin filaments. J Cell Biol 106: 1545–1552
Kamitsubo E (1966) Motile protoplasmic fibrils in cells of Characeae II: linear fibrillar structure and its bearing on protoplasmic streaming. Proc Jpn Acad 42: 640–643
— (1972) Motile protoplasmic fibrils in cells of the Characeae. Protoplasma 74: 53–70
Kamiya N (1981) Physical and chemical basis of cytoplasmic streaming. Annu Rev Plant Physiol 32: 205–236
— (1986) Cytoplasmic streaming in giant algal cells: a historical survey of experimental approaches. Bot Mag Tokyo 99: 441–467
—, Kuroda K (1956) Velocity distribution of the protoplasmic streaming inNitella cells. Bot Mag Tokyo 69: 544–550
Kato T, Tonomura Y (1977) Identification of myosin inNitella flexilis. J Biochem 82: 777–782
Kersey YM, Wessells NK (1976) Localization of actin filaments in internodal cells of characean algae: a scanning and transmission electron microscope study. J Cell Biol 68: 264–275
—, Hepler PK, Palevitz BA, Wessells NK (1976) Polarity of actin filaments in characean algae. Proc Natl Acad Sci USA 73: 165–167
Kikuyama M, Tazawa M (1982) Ca2+ ion reversibly inhibits the cytoplasmic streaming ofNitella. Protoplasma 113: 241–243
Kinkema M, Schiefelbein J (1994) A myosin from a higher plant has structural similarities to class V myosins. J Mol Biol 239: 591–597
—, Wang H, Schiefelbein J (1994) Molecular analysis of the myosin gene family inArabidopsis thaliana. Plant Mol Biol 26: 1139–1153
Knight AE, Kendrick-Jones J (1993) A myosin-like protein from a higher plant. J Mol Biol 231: 148–154
Kohno T, Shimmen T (1988a) Accelerated sliding of pollen tube organelles along Characeae actin bundles regulated by Ca2+. J Cell Biol 106: 1539–1543
— — (1988b) Mechanism of Ca2+ inhibition of cytoplasmic streaming in lily pollen tubes. J Cell Sci 91: 501–509
—, Chaen S, Shimmen T (1990) Characterization of the translocator associated with pollen tube organelles. Protoplasma 154: 179–183
—, Okagaki T, Kohama K, Shimmen T (1991) Pollen tube extract supports the movement of actin filaments in vitro. Protoplasma 161: 75–77
—, Ishikawa T, Nagata T, Kohama K, Shimmen T (1992) Partial purification of myosin from lily pollen tubes by monitoring with in vitro motility assay. Protoplasma 170: 77–85
Kron SJ, Spudich JA (1986) Fluorescent actin filaments move on myosin fixed to a glass surface. Proc Natl Acad Sci USA 83: 6272–6276
La Claire JW II (1991) Immunolocalization of myosin in intact and wounded cells of the green algaErnodesmis verticillata (Klitzing) Børgesen. Planta 184: 209–217
Lichtscheidl IK, Lancelle SA, Hepler PK (1990) Actin-endoplasmic reticulum complexes in Drosera: their structural relationship with the plasmalemma, nucleus, and organelles in cells prepared by high pressure freezing. Protoplasma 155: 116–126
Liebe S, Quader H (1994) Myosin in onion (Allium cepa) bulb scale epidermal cells: involvement in dynamics of organelles and endoplasmic reticulum. Physiol Plant 90: 114–124
Lin Q, Grolig F, Jablonsky PP, Williamson RE (1989) Myosin heavy chains: detection by immunoblotting in higher plants and localization by immunofluorescence in the algaChara. Cell Biol Int Rep 13: 107–117
—, Jablonsky PP, Elliot J, Williamson RE (1994) A 170 kDa polypeptide from mung bean shares multiple epitopes with rabbit skeletal myosin and binds ADP-agarose. Cell Biol Int 18: 1035–1047
Lorz H, Paszkowski J, Dierks-Ventling C, Potrykus I (1981) Isolation and characterization of cytoplasts and miniprotoplasts derived from protoplasts of cultured cells. Physiol Plant 53: 385–391
Lupas A, Van Dyke M, Stock J (1991) Predicting coiled coils from protein sequences. Science 252: 1162–1164
Ma Y-Z, Yen L-F (1989) Actin and myosin in pea tendrils. Plant Physiol 89: 586–589
McCurdy DW, Harmon AC (1992a) Calcium-dependent protein kinase in the green algaChara. Planta 188: 54–61
— — (1992b) Phosphorylation of a putative myosin light chain inChara by calcium-dependent protein kinase. Protoplasma 171: 85–88
Mermall V, Post PL, Mooseker MS (1998) Unconventional myosins in cell movement, membrane traffic, and signal transduction. Science 279: 527–533
Miller DD, Callaham DA, Gross DJ, Hepler PK (1992) Free Ca2+ gradient in growing pollen tubes ofLilium. J Cell Sci 101: 7–12
—, Scordillis SP, Hepler PK (1995) Identification and localization of three classes of myosins in pollen tubes ofLilium longiflorum andNicotiana alata. J Cell Sci 108: 2549–2553
Moepps B, Conrad S, Schraudolf H (1993) PCR-dependent amplification and sequence characterization of partial cDNAs encoding myosin-like proteins inAnemia phyllitidis (L.) Sw. andArabidopsis thaliana (L.) Heynh. Plant Mol Biol 21: 1077–1083
Nagai R, Hayama T (1979) Ultrastructure of the endoplasmic factor responsible for cytoplasmic streaming inChara internodal cells. J Cell Sci 36: 121–136
—, Rebhun LI (1966) Cytoplasmic microfilaments in streamingNitella cells. J Ultrastruct Res 14: 571–589
Nothnagel EA, Webb WW (1982) Hydrodynamic models of viscous coupling between motile myosin and endoplasm in characean algae. J Cell Biol 94: 444–454
Ohsuka K, Inoue A (1979) Identification of myosin in a flowering plant,Egeria densa. J Biochem 85: 375–378
Palevitz BA, Hepler PK (1975) Identification of actin in situ at the ectoplasm-endoplasm interface of Nitella: microfilament-chloroplast association. J Cell Biol 65: 29–38
—, Ash JF, Hepler PK (1974) Actin in the green alga,Nitella. Proc Natl Acad Sci USA 71: 363–366
Parke J, Miller C, Anderton BH (1986) Higher plant myosin heavy-chain identified using a monoclonal antibody. Eur J Cell Biol 41: 9–13
Pierson ES, Miller DD, Callaham DA, Shipley AM, Rivers BA, Cresti M, Hepler PK (1994) Pollen tube growth is coupled to the extracellular calcium ion flux and the intracellular calcium gradient: effect of BAPTA-type buffers and hypertonic media. Plant Cell 6: 1815–1828
— — —, van Aken J, Hackett G, Hepler PK (1996) Tip-localized calcium entry fluctuates during pollen tube growth. Dev Biol 174: 160–173
Plazinski J, Elliott J, Hurley UA, Burch J, Arioli T, Williamson RE (1997) Myosins from angiosperms, ferns, and algae: amplification of gene fragments with versatile PCR primers and detection of protein products with a monoclonal antibody to a conserved head epitope. Protoplasma 196: 78–86
Quader H, Hofmann A, Schnepf E (1987) Shape and movement of the endoplasmic reticulum in onion bulb epidermis cells: possible involvement of actin. Eur J Cell Biol 44: 17–26
Radford JE, White RG (1998) Localization of a myosin-like protein to plasmodesmata. Plant J 14: 743–750
Rathore KS, Cork RJ, Robinson KR (1991) A cytoplasmic gradient of Ca2+ is correlatd with the growth of lily pollen tubes. Dev Biol 148: 612–619
Reiss H-D, Nobling R (1986) Quin-2 fluorescence in lily pollen tubes: distribution of free cytoplasmic calcium. Protoplasma 131: 244–246
Rhoads AR, Friedberg F (1997) Sequence motifs for calmodulin recognition. FASEB J 11: 331–340
Sheetz MP, Spudich JA (1983) Movement of myosin-coated fluorescent beads on actin cables in vitro. Nature 303: 31–35
Shimmen T (1978) Dependency of cytoplasmic streaming on intracellular ATP and Mg2+ concentrations. Cell Struct Fund 3: 113–121
— (1988) Cytoplasmic streaming regulated by adenine nucleotides and inorganic phosphates in Characeae. Protoplasma Suppl 1: 3–9
— (1992) Inhibitory regulation of cytoplasmic streaming by Ca2+ in plant cells. In: Kohama K (ed) Calcium inhibition. Japan Scientific Societies Press and CRC Press, Tokyo and Boca Raton, pp 69–90
—, Tazawa M (1982) Reconstitution of cytoplasmic streaming in Characeae. Protoplasma 113: 127–131
— — (1983) Control of cytoplasmic streaming by ATP, Mg2+, and cytochalasin B in permeabilized Characeae cell. Protoplasma 115: 18–24
—, Yano M (1986) Regulation of myosin sliding alongChara actin bundles by native skeletal muscle tropomyosin. Protoplasma 132: 129–136
—, Xu Y-L, Kohno T (1990) Inhibition of cytoplasmic streaming by sulphate in characean cells. Protoplasma 159: 39–44
Sibata-Sekiya K, Tonomura Y (1975) Desensitization of substrate inhibition of acto-H-meromyosin ATPase by treatment of H-meromyosin withp-chloromercuribenzoate: relation between extent of desensitization and amount of boundp-chloromercuribenzoate. J Biochem 77: 543–557
Sokolov OI, Bogatyrev VA, Turkina MV (1986) Myosin from conducting tissues ofHeracleum sosnowskyi: interaction with muscle actin and formation of filaments. Fiziol Rast 33: 421–431
Sonobe S (1996) Studies on the plant cytoskeleton using miniprotoplasts of tobacco BY-2 cells. J Plant Res 109: 437–448
Takagi S, Yokota E, Shimmen T, Nagai R (1995) Motor protein activity for cytoplasmic streaming detected inVallisneria leaves. Plant Cell Physiol 36: S132
Tang X, Hepler PK, Scordilis SP (1989) Immunochemical and immunocytochemical identification of a myosin heavy chain polypeptide inNicotiana pollen tubes. J Cell Sci 92: 569–574
Tazawa M (1964) Studies onNitella having artificial cell sap 1: replacement of the cell sap with artificial solutions. Plant Cell Physiol 5: 33–43
—, Kishimoto U (1968) Cessation of cytoplasmic streaming ofChara internodes during action potential. Plant Cell Physiol 9: 361–368
—, Kikuyama M, Shimmen T (1976) Electric characteristics and cytoplasmic streaming of Characeae cells lacking tonoplast. Cell Struct Funct 1: 165–176
Terasaki O, Niitsu T (1994) Differential roles of microtubule and actin-myosin cytoskeleton in the growth ofPinus pollen tubes. Sex Plant Reprod 7: 264–272
Tischendorf G, Sawitzky D, Werz G (1987) Antibodies specific for vertebrate actin, myosin, actinin, or vincuylin recognize epitopes in the giant nucleus of the marine green algaAcetabularia. Cell Motil Cytoskeleton 7: 78–86
Tominaga Y, Shimmen T, Tazawa M (1983) Control of cytoplasmic streaming by extracellular Ca2+ in permeabilizedNitella cells. Protoplasma 116: 75–77
—, Wayne R, Tung HYL, Tazawa M (1987) Phosphorylation-dephosphorylation is involved in Ca2+-controlled cytoplasmic streaming of characean cells. Protoplasma 136: 161–169
Tonomura Y, Yoshimura J (1962) Binding ofp-chloromercuribenzoate to actin. J Biochem 51: 259–266
Uyeda TQP (1996) Ultra-fastChara myosin: a test case for the swinging lever arm model for force production by myosin. J Plant Res 109: 231–239
Vahey M, Titus M, Trautwein R, Scordilis S (1982) Tomato actin and myosin: contractile proteins from a higher land plant. Cell Motil 2: 131–147
Williamson RE (1974) Actin in the alga,Chara corallina. Nature 248: 801–802
— (1975) Cytoplasmic streaming inChara: a cell model activated by ATP and inhibited by cytochalasin B. J Cell Sci 17: 655–668
— (1976) Cytoplasmic streaming in characean algae. In: Wardlaw IF, Passioura JB (eds) Transport and transfer processes in plants. Academic Press, New York, pp 51–58
— (1979) Filaments associated with the endoplasmic reticulum in the streaming cytoplasm ofChara corallina. Eur J Cell Biol 20: 177–183
— (1993) Organelle movements. Annu Rev Plant Physiol Plant Mol Biol 44: 181–202
—, Ashley CC (1982) Free Ca2+ and cytoplasmic streaming in the algaChara. Nature 296: 647–651
Yamaguchi M, Nakamura T, Sekine T (1973) Studies on the fast reacting sulfhydryl group of skeletal myosin A: conversion to smooth muscle myosin type with N-ethylmaleimide treatment. Biochim Biophys Acta 328: 154–165
Yamamoto K, Kikuyama M, Sutoh-Yamamoto N, Kamitsubo E (1994) Purification of actin based motor protein fromChara corallina. Proc Jpn Acad Ser B 70: 175–180
— — — —, Katayama E (1995) Myosin from alga Chara: unique structure revealed by electron microscopy. J Mol Biol 254: 109–112
Yokota E, Shimmen T (1994) Isolation and characterization of plant myosin from pollen tubes of lily. Protoplasma 177: 153–162
Yokota E, McDonald AR, Liu B, Shimmen T, Palevitz BA (1995a) Localization of a 170 kDa myosin heavy chain in plant cells. Protoplasma 185: 178–187
—, Mimura T, Shimmen T (1995b) Biochemical, immunochemical and immunohistochemical identification of myosin heavy chain in cultured cells ofCatharanthus roseus. Plant Cell Physiol 36: 1541–1547
—, Muto S, Shimmen T (1999a) Inhibitory regulation of higher-plant myosin by Ca2+ ions. Plant Physiol 119: 231–239
—, Yukawa C, Muto S, Sonobe S, Shimmen T (1999b) Biochemical and immunocytochemical characterization of two types of myosins in cultured tobacco bright yellow-2 cells. Plant Physiol 121: 525–534
Yoneda M, Nagai R (1988) Structural basis of cytoplasmic streaming in characean internodal cells: hydrodynamic analysis. Protoplasma 147: 64–76
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Dedicated to the memory of the late Professor Noburo Kamiya (1913–1999)
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Shimmen, T., Ridge, R.W., Lambiris, I. et al. Plant myosins. Protoplasma 214, 1–10 (2000). https://doi.org/10.1007/BF02524256
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DOI: https://doi.org/10.1007/BF02524256