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An improved method for rapid analysis of the fatty acids of glycerolipids

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Lipids

An Erratum to this article was published on 01 August 1996

Abstract

An improved rapid procedure to determine the fatty acid composition of glycerolipids is described. The procedure includes KOH-catalyzed transesterification and high-speed gas chromatography. Glycerolipids (20–40 mg) were mixed with 2 mL of hexane and 0.2 mL of 2 M methanolic KOH at room temperature for 1–2 min. The fatty acid methyl esters in the hexane layer were analyzed by gas chromatography on 10% SP-2340 at 240°C. Methyl linolenate and docosahexaenoate eluted within 2 and 5 min, respectively. Analysis was thus completed with 5 min for common vegetable oils and 8 min for fish oils.

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Abbreviations

DEGS:

diethylene glycol succinate polyester

DGDG:

digalactosyldiacylglycerol

FA:

fatty acid

FAME:

fatty acid methyl ester

GC:

gas chromatography

PC:

phosphatidylcholine

TG:

triacylglycerol

TLC:

thin-layer chromatography

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Author information

Authors and Affiliations

  1. Department of Agricultural Chemistry, Kyoto Prefectural University, 606, Kyoto, Japan

    Ken’ichi Ichihara

  2. Department of Clinical Nutrition, Osaka Prefectural College of Health Sciences, 583, Habikino, Japan

    Akira Shibahara

  3. General Testing Research, Institute of Japan Oil Stuff Inspectors’ Corporation, 658, Kobe, Japan

    Kohei Yamamoto & Takao Nakayama

Authors
  1. Ken’ichi Ichihara
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  2. Akira Shibahara
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  3. Kohei Yamamoto
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  4. Takao Nakayama
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Additional information

An erratum to this article is available at http://dx.doi.org/10.1007/BF02522986.

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Ichihara, K., Shibahara, A., Yamamoto, K. et al. An improved method for rapid analysis of the fatty acids of glycerolipids. Lipids 31, 535–539 (1996). https://doi.org/10.1007/BF02522648

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  • DOI: https://doi.org/10.1007/BF02522648

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